Next Article in Journal
Natural Course of Metabolically Healthy Overweight/Obese Subjects and the Impact of Weight Change
Next Article in Special Issue
Curcumin AntiCancer Studies in Pancreatic Cancer
Previous Article in Journal
Food Consumption and Nutrient Intake by Children Aged 10 to 48 Months Attending Day Care in The Netherlands
Previous Article in Special Issue
Mangiferin and Cancer: Mechanisms of Action
Open AccessArticle

Quercitrin from Toona sinensis (Juss.) M.Roem. Attenuates Acetaminophen-Induced Acute Liver Toxicity in HepG2 Cells and Mice through Induction of Antioxidant Machinery and Inhibition of Inflammation

by Van-Long Truong 1,†, Se-Yeon Ko 1,†, Mira Jun 2 and Woo-Sik Jeong 1,*
1
Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, Korea
2
Department of Food and Science & Nutrition, Dong-A University, Busan 49315, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2016, 8(7), 431; https://doi.org/10.3390/nu8070431
Received: 30 May 2016 / Revised: 29 June 2016 / Accepted: 11 July 2016 / Published: 15 July 2016
(This article belongs to the Special Issue Polyphenols for Cancer Treatment or Prevention)
Quercitrin is found in many kinds of vegetables and fruits, and possesses various bioactive properties. The aim of the present study was to elucidate hepatoprotective mechanisms of quercitrin isolated from Toona sinensis (Juss.) M.Roem. (syn. Cedrela sinensis Juss.), using acetaminophen (APAP)-treated HepG2 cell and animal models. In an in vitro study, quercitrin suppressed the production of reactive oxygen species and enhanced expression of nuclear factor E2-related factor 2 (Nrf2), activity of antioxidant response element (ARE)-reporter gene, and protein levels of NADPH: quinone oxidoreductase 1 (NQO1), catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase 2 (SOD-2) in APAP-treated HepG2 cells. In an in vivo study, Balb/c mice were orally administered with 10 or 50 mg/kg of quercitrin for 7 days and followed by the injection with single dose of 300 mg/kg APAP. Quercitrin decreased APAP-caused elevation of alanine aminotransferase and aspartate aminotransferase levels, liver necrosis, the expression of pro-inflammatory factors including inducible nitric oxide synthase, cyclooxygenase 2 and inerleukin-1β, and phosphorylation of kinases including c-Jun N-terminal kinase and p38. Quercitrin restored protein levels of Nrf2, NQO1 and activities and expressions of CAT, GPx, SOD-2. The results suggested that quercitrin attenuates APAP-induced liver damage by the activation of defensive genes and the inhibition of pro-inflammatory genes via the suppressions of JNK and p38 signaling. View Full-Text
Keywords: quercitrin; acetaminophen; hepatoprotection; Nrf2/ARE; antioxidant quercitrin; acetaminophen; hepatoprotection; Nrf2/ARE; antioxidant
Show Figures

Figure 1

MDPI and ACS Style

Truong, V.-L.; Ko, S.-Y.; Jun, M.; Jeong, W.-S. Quercitrin from Toona sinensis (Juss.) M.Roem. Attenuates Acetaminophen-Induced Acute Liver Toxicity in HepG2 Cells and Mice through Induction of Antioxidant Machinery and Inhibition of Inflammation. Nutrients 2016, 8, 431.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop