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Nutrients 2016, 8(6), 359; doi:10.3390/nu8060359

Review
Protein Consumption and the Elderly: What Is the Optimal Level of Intake?
1
Department of Food Science, University of Arkansas, 2650 N. Young Ave, Fayetteville, AR 72704, USA
2
Department of Geriatrics, the Center for Translational Research on Aging and Longevity, Donald W. Reynolds Institute on Aging, College of Medicine, The University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
*
Author to whom correspondence should be addressed.
Received: 26 May 2016 / Accepted: 3 June 2016 / Published: 8 June 2016

Abstract

:
Maintaining independence, quality of life, and health is crucial for elderly adults. One of the major threats to living independently is the loss of muscle mass, strength, and function that progressively occurs with aging, known as sarcopenia. Several studies have identified protein (especially the essential amino acids) as a key nutrient for muscle health in elderly adults. Elderly adults are less responsive to the anabolic stimulus of low doses of amino acid intake compared to younger individuals. However, this lack of responsiveness in elderly adults can be overcome with higher levels of protein (or essential amino acid) consumption. The requirement for a larger dose of protein to generate responses in elderly adults similar to the responses in younger adults provides the support for a beneficial effect of increased protein in older populations. The purpose of this review is to present the current evidence related to dietary protein intake and muscle health in elderly adults.
Keywords:
protein; aging; muscle; requirements; anabolic response; protein synthesis; elderly

1. Introduction

The United States is experiencing considerable growth in its elderly adult population. By 2015, the population aged 65 and over is projected to reach nearly 84 million [1]. Maintaining independence, quality of life, and health is crucial for elderly adults [2]. One of the major threats to living independently is the loss of muscle mass, strength, and function that progressively occurs with aging, known as sarcopenia [2,3]. A loss or reduction in skeletal muscle function often leads to increased morbidity and mortality either directly, or indirectly, via the development of secondary diseases such as cardiovascular disease, diabetes, and obesity [3,4]. The prevalence of obesity among elderly adults has also increased over the last several decades. For example, the prevalence of obesity among men aged 65–74 increased from 31.6% in 1999–2002 to 41.5% in 2007–2010. Between 2007 and 2010, approximately 35% of adults aged 65 and over were obese [5]. One reason for the increase in obesity could be due to body composition shifts that occur as we age, resulting in a higher percentage of body fat and decreases in muscle mass with age [6]. Both sarcopenia and obesity act synergistically, which increases the risk of negative health outcomes and earlier onset of disability [2].
Nutrition plays an essential role in the health and function of elderly adults [7]. Inadequate nutrition can contribute to the development of both sarcopenia and obesity [3,8]. As life expectancy continues to rise, it is important to consider optimal nutritional recommendations that will improve health outcomes, quality of life, and physical independence in elderly adults [5]. Several studies have identified protein as a key nutrient for elderly adults (reviewed in [3,8]). Protein intake greater than the recommended amounts may improve muscle health, prevent sarcopenia [9], and help maintain energy balance, weight management [10], and cardiovascular function [11,12,13]. Benefits of increased protein intake include improved muscle function and the prevention onset of chronic diseases, which can increase quality of life in healthy elderly adults [3]. Therefore, the purpose of this review is to present the current evidence related to dietary protein intake and muscle health in elderly adults.

2. Optimal Protein Intake for Elderly Adults

2.1. Dietary Protein Recommendations

Traditionally, protein recommendations have been based on studies that estimate the minimum protein intake necessary to maintain nitrogen balance [3,8]. However, the problem with relying on these results is that they do not measure any physiological endpoints relevant to healthy aging, such as muscle function. The current dietary recommendations for protein intake include the dietary reference intakes (DRI) for macronutrients, which include an estimated average requirement (EAR), a recommended dietary allowance (RDA) and an acceptable macronutrient distribution range (AMDR) [14]. In the case of daily protein intake, the EAR for dietary protein is 0.66 g/kg/day and the Food and Nutrition Board recommends an RDA of 0.8 g/kg/day for all adults over 18 years of age, including elderly adults over the age of 65. The RDA for protein was based on all available studies that estimate the minimum protein intake necessary to avoid a progressive loss of lean body mass as determined by nitrogen balance [3,8]. The Food and Nutrition Board recognizes a distinction between the RDA and the level of protein intake needed for optimal health. Therefore, the recommendation for the ADMR includes a range of optimal protein intakes in the context of a complete diet (10%–35% of daily energy intake come from protein [14]), which makes the ADMR more relevant to normal dietary intake than the RDA [3].

2.2. Protein Requirements for Elderly Adults

Experts in the field of protein and aging recommend a protein intake between 1.2 and 2.0 g/kg/day or higher for elderly adults [3,8,15]. The RDA of 0.8 g/kg/day is well below these recommendations and reflects a value at the lowest end of the AMDR. It is estimated that 38% of adult men and 41% of adult women have dietary protein intakes below the RDA [16,17].
Most published results, based on data from either epidemiological or short-term studies, indicate a potential beneficial effect of increasing protein intake in elderly adults. These data demonstrate that elderly adults, compared with younger adults, are less responsive to low doses of amino acid intake [18]. However, this lack of responsiveness in healthy older adults can usually be overcome with higher levels of essential amino acid (EAA) consumption [18]. This is also reflected in studies comparing varying levels of protein consumption [19], suggesting that the lack of muscle responsiveness to lower doses of protein intake in elderly adults can be overcome with a higher level of protein intake. The requirement for a larger dose of protein to generate responses in elderly adults similar to the responses in younger adults provides the support for a beneficial effect of increased protein in older populations [8].
The mechanism by which dietary protein affects muscle is through the stimulation of muscle protein synthesis and/or suppression of protein breakdown by the absorbed amino acids consumed in the diet [20,21]. There appears to be an EAA threshold when it comes to stimulating muscle protein synthesis. Ingestion of relatively small amounts of EAA (2.5, 5 or 10 g) appears to increase myofibrillar protein synthesis in a dose-dependent manner [22]. However, a larger dose of EAA (20–40 g) fail to elicit an additional effect on protein synthesis in young and older subjects. Similar results were observed after the ingestion of either 113 or 340 g of lean beef containing 10 or 30 g EAA, respectively [23]. Despite a threefold increase in EAA content, there was no further increase in protein synthesis in either young or older subjects following consumption of 340 g versus 113 g of protein. There are fewer data regarding the response of protein breakdown to different levels of protein or amino acid intake. The balance between protein synthesis and breakdown is discussed in more detail below.

2.3. Essential Amino Acid Requirements for Aging Adults

Essential amino acids, especially the branched-chain amino acid leucine, are potent stimulators of muscle protein synthesis. Studies have focused on the stimulation of muscle protein synthesis via the protein kinase mTORC1 (mechanistic target of rapamycin complex 1) [24,25,26], but the in vivo significance of this mechanism as a regulator of the rate of protein synthesis in human subjects is not yet proven. Several studies demonstrate that maximal stimulation of muscle protein synthesis is possible with 15 g of EAA (reviewed in [20]). This translates to ~35 g of high quality protein per meal delivering ~15 g of EAA. A larger amount of lower quality protein, which contains a lower content of EAA, would be required to achieve the same functional benefits. The addition of nonessential amino acids to a supplement containing EAA does not result in additional stimulation of muscle protein synthesis [27], indicating that the quality of the protein, or its amino acid profile, is a key determinant of the functional potential of protein in muscle health. This is supported by several studies demonstrating that the ingestion of milk proteins, compared with the ingestion of soy protein stimulates muscle protein synthesis to a greater extent after resistance exercise, owing to the higher content of EAA in milk protein [28,29,30,31]. The data from the Health, Aging and Body Composition study support these findings [31], showing that intake of animal protein (with greater content of EAA), but not plant protein, was significantly associated with the preservation of lean body mass over three years in older adults [31]. In that study, individuals in the highest quintile of protein intake had 40% less loss in lean body mass than those in the lowest quintile of protein intake [31].

2.4. The Importance of Protein Quality

When considering protein intake, it is also important to consider total energy intake. Age is associated with a progressive decline in basal metabolic rate (BMR) at a rate of 1%–2% per decade after 20 years of age [32,33,34]. This reduction in BMR is closely associated with the loss in fat-free mass, including muscle, and the gain of less metabolically active fat [35] that occurs as we age [33]. In fact, studies suggest that BMR adjusted for the change in fat-free mass is 5% lower in elderly adults compared to younger adults [35]. This implies that aging adults require a lower daily energy intake. However, the extent to which BMR may increase or decrease with age depends on the balance between weight gain with age, tending to increase BMR, and aging, which decreases BMR [35].
Although older adults typically eat less than younger adults, including less protein [15,16], it is important for aging adults to consider total caloric intake when choosing a protein source to incorporate in the diet. The discrepancies in quality between animal and plant protein sources go beyond the amino acid profiles. When the energy content of the protein source is accounted for, the caloric intake needed to meet the EAA requirements from plant sources of protein is considerably higher than the caloric intake from animal sources of protein [36]. This is important to consider since obesity, especially with aging, is a major public health concern. Obesity is the most predominant factor limiting mobility in the elderly [37].

2.5. Dietary Protein and Muscle Anabolic Response in Elderly Adults

There is abundant evidence that muscle plays a central role in the prevention of many chronic diseases, including diabetes and obesity [38]. In addition, evidence that optimal health for elderly adults is dependent on maintaining muscle mass is emerging [3,8]. EAAs are the primary nutrients responsible for the maintenance of muscle mass and function, but elderly individuals have reduced anabolic sensitivity to amino acids (termed anabolic resistance). An increasing amount of evidence suggests that a minimum threshold of EAA needs to be reached to elicit an anabolic muscle response, and older individuals require a higher concentration of amino acids compared to younger individuals.
Optimal protein intake per meal can be defined as the minimal dose of protein intake that results in the maximal anabolic response and thus can help maintain or improve muscle mass (reflected as lean body mass) and function over time. It has been reported that the optimal dose of dietary protein consumption in a meal that results in a near maximal anabolic response is ~35 g/meal [23] or 0.40 g/kg/meal of high-quality protein in elderly adults [19], translatable to 1.2 g/kg/day or 96 g/day for an 80 kg elderly adults. The optimum amount for elderly adults (0.24 g/kg/meal) is approximately 70% greater than that for young adults (0.8 g/kg/day) [19], indicating an age-associated anabolic resistance to dietary protein. It is likely that elderly individuals need more protein intake to achieve a maximal anabolic response per meal considering the varying degrees of quality of protein eaten in the real world. In a typical American diet, the consumption of the majority of total daily protein intake skews toward dinner (~50% of total amount; ~40–60 g protein) [16,17,39] that clearly exceeds the “optimal” protein dose (i.e., ~35 g protein/meal) without extra stimulation of anabolic response. This led to an interesting hypothesis that spreading daily protein intake evenly throughout the day can result in a greater cumulative anabolic response than the skewed pattern of protein intake [40]. If this is the case, elderly adults can gain benefits regarding improvement in muscle mass and strength, and related functions, simply by adopting even distribution pattern of equal amounts of protein intake [40]. However, the rationale behind this hypothesis is largely incorrect, as the hypothesis was solely based on data on muscle protein synthesis (MPS), which is only one half of the equation determining net anabolic response (i.e., net anabolic response = protein synthesis minus protein breakdown).
The significance of simultaneous measurement of both protein synthesis and breakdown is dependent on a number of catabolic conditions (i.e., loss of muscle mass over time) such as type I diabetes, cancer cachexia, and burn injury, in which the rate of protein synthesis is typically not blunted but actually normal or often increased [41], due largely to the increased availability of amino acids secondary to an accelerated rate of protein breakdown. This issue is important when quantifying the net anabolic response to dietary protein intake. Furthermore, although net anabolic response at the muscle level is the most relevant physiological response, the whole body is potentially involved in the anabolic response to protein ingestion, as approximately half of the total body protein turnover occurs at non-muscle tissues, particularly gut tissue [42]. Thus, determination at the muscle level could underestimate total anabolic response. For example, a large portion of the amino acids absorbed from a meal is retained in gut proteins that turn over rapidly [42,43], particularly following a mixed meal, due largely to a systemic insulin response [42]. Those amino acids can, in turn, be released into the blood over time as a result of a protein breakdown and be used for incorporation into new proteins in muscle. This is of particular importance in situations where older adults consume a protein intake greater than the amount that stimulates a maximal MPS.
Consistent with this notion, our recent findings showed that similar MPS responses were achieved by two doses of protein intake (40 g vs. 70 g), while a greater net protein synthesis at whole-body level was achieved with a meal containing 70 g of protein due to the suppression of breakdown amplifying the anabolic effect of the stimulation of synthesis [44]. Furthermore, we have directly tested the “distribution” hypothesis at two protein levels (0.8 g or 1.5 g protein/kg/day) in mixed meals and found no beneficial effects of an even distribution pattern of protein intake on net anabolic response at whole-body level and MPS [45]. Instead, we found the higher protein intake (i.e., 1.5 g/kg/day) resulted in a greater anabolic response at whole-body level and MPS. Strikingly, the positive anabolic response achieved with both levels of protein intake was largely due to reductions in protein breakdown, indicating the importance of simultaneous determination of both protein synthesis and breakdown, as protein synthesis actually declined with 0.8 g protein/kg/day, regardless of the distribution patterns. Furthermore, the same study [45] showed that whole body anabolic response increased linearly with increasing amount of protein intake (dose range: ~6.4–91.7 g), without evidence of plateau in older adults [45]. These results extended previous findings shown by the Deutz group [46,47], indicating that the amount of total protein, but not the pattern of protein intake, is of importance with respect to maximizing anabolic response. Importantly, the linear relationship between the amount of protein intake and anabolic response has been recognized for more than half a century, as determined by a nitrogen balance technique, although the anabolic response beyond RDA for protein (i.e., 0.8 g protein/kg/day) has been ignored [48]. Therefore, data indicate that there is no practical limit to the anabolic response in increasing amount of dietary protein intake.
Taken together, the data do not support the notion that a maximal anabolic response is stimulated with ~35 g of high quality protein per meal [23] or 0.4 g/kg/meal (1.2 g/kg/day) for older adults [19]. The “even distribution hypothesis” was based on this limit of anabolic response [40], but that hypothesis ignored many important factors in determining true net anabolic response. These factors include the quality of protein consumed, the contribution of protein breakdown to the net anabolic response, and the potential involvement of whole body response, all of which result in the considerable underestimation of the maximal anabolic response. It is therefore unreasonable to base recommendations for the optimal level of protein intake in elderly adults on the idea that the maximal effective dose of protein is ~35 g per meal. If the goal of the optimal level of protein intake is considered to be the amount needed to maximally stimulate protein anabolism (i.e., synthesis minus breakdown), then consumption of dietary protein in accord with the higher end of the AMDR (35% of total calories) is reasonable. Unfortunately, long-term studies assessing the effect of this level of dietary protein consumption on functional outcomes in elderly adults have not been performed.

2.6. Dietary Protein and Anabolic Signaling in Muscle of Elderly Adults

Signaling through mTORC1 is involved in the regulation of several anabolic processes in the body including protein synthesis [26,49,50]. In skeletal muscle, amino acids signal through mTORC1 to initiate the process of protein synthesis [25,51,52,53]. The translation initiation factors 4E-BP1 (eukaryotic initiation factor 4E binding protein 1) and p70S6K (ribosomal protein S6 kinase) are downstream targets of mTORC1 [51,52,53]. Signals provided by EAA, especially leucine, are required for full activation of this pathway [25,51,54]. Muscle becomes resistant to the normal stimulatory effects of postprandial leucine concentrations with increasing age [18], which may result in the reduced stimulation of the mTORC1 pathway and reduced activation of translation initiation and subsequent MPS. This could be due to a reduced sensitivity to leucine with age, to less efficient absorption of leucine from the gut, or to the fact that the dietary protein intake tends to decrease with age [8,55,56].
Age-related muscle loss may involve a decreased response to EAA due to decreased phosphorylation of mTORC1 and p70S6K [22]. In response to 10 g of EAA, mTORC1 phosphorylation, or activation, while significantly increased in skeletal muscle of elderly adults, is still significantly lower in younger adults [22]. Guillet et al. [57] found that p70S6K phosphorylation is not stimulated in older adults after infusion with leucine. These findings are supported by Fry et al. [58] who found that elderly adults, compared with young adults, have significantly reduced phosphorylation of mTORC1 and translation initiation factors after a bout of resistance exercise. Gene expression of proteins associated with muscle protein synthesis and satellite cell function also differ between young and elderly adults in response to exercise and supplementation with EAA [59]. While no difference was found between young and elderly in the fasted state, there was a significant decrease in protein (REDD1, TSC1, TSC2, and IGF1 receptor) expression six hours post-exercise and EAA intervention in elderly adults versus young adults [59]. In addition, after only seven days of bed rest, elderly adults had a reduced response to EAA ingestion resulting in no increase in MPS, activation of translation initiation factors (4E-BP1 and p70S6K), and no increase in amino acid transporters [60]. Elderly adults also had decreased LAT1 (L-type amino acid transporter) and SNAT2 (sodium-coupled neutral amino acid transporter 2) following seven days of bed rest [60]. These findings are further supported in a study by Tanner et al. [61], who found that, after five days of bed rest, elderly adults (but not younger adults) had reduced amino acid-induced anabolic sensitivity, resulting in decreased muscle protein synthesis. In this study, elderly adults had increased MURF1 gene expression at baseline and increased AMPKα phosphorylation after bed rest, which is suggestive of increased muscle protein breakdown [61]. These data are important because they demonstrate how quickly an injury or hospital stay could decrease skeletal muscle function. While all of these data suggest a potential role of changes in sensitivity of mTORC1 and related factors in the anabolic response as well as anabolic resistance in elderly adults, it must also be acknowledged that the nature of the data is correlational and thus does not definitively prove a cause–effect relationship. To this end, it has recently been shown that consumption of a very small dose of EAA (3 g) can stimulate muscle protein anabolism equivalently to 20 g of whey protein in the absence of any time-coincident changes in initiation factor activity [62].

3. Conclusions

Elderly adults are less responsive to the anabolic stimulus of low doses of amino acid intake compared to younger adults [18]. However, this lack of responsiveness in elderly adults can be overcome with higher levels of protein consumption [18]. This is also reflected in studies comparing varying levels of protein intake [19]. This suggests that the lack of muscle responsiveness to lower doses of protein in older adults can be overcome with a higher level of protein intake. The requirement for a larger dose of protein to generate responses in elderly adults similar to the responses in younger adults provides the support for a beneficial effect of increased protein in elderly populations [8]. The consumption of dietary protein consistent with the upper end of the AMDRs (as much as 30%–35% of total caloric intake) may prove to be beneficial, although practical limitations may make this level of dietary protein intake difficult. The consumption of high-quality proteins that are easily digestible and contain a high proportion of EAAs lessens the urgency of consuming diets with an extremely high protein content.

Acknowledgments

The authors were supported by the Claude D. Pepper Center for Older Americans in Little Rock, AR. Baum and Kim were supported by a Pepper Center Pilot Study Award P30 AG028718. Wolfe has received honoraria for talks or consulting from the National Cattleman’s Beef Association, PepsiCo, and Pronutria. Wolfe has also received research grants for the Abbott Nutrition and National Cattleman’s Beef Association. Baum has received grants from the Egg Nutrition Center/American Egg Board.

Author Contributions

The authors wrote and reviewed the material together.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

The following abbreviations are used in this manuscript:
4E-BP1eukaryotic initiation factor 4E-binding protein 1
AMDRacceptable macronutrient distribution range
AMPKAMP-activated protein kinase
BMRbasal metabolic rate
DRIdietary reference intake
EAAessential amino acids
EARestimated average requirement
LAT1L-type amino acid transporter
MPSmuscle protein synthesis
mTORC1mechanistic target of rapamycin
MURF1muscle RING-finger protein-1
p70S6Kribosomal protein S6 kinase
RDArecommended dietary allowance
REDD1regulated in development and DNA damage responses 1
SNAT2sodium-coupled neutral amino acid transporter 2
TSC1tuberous sclerosis 1
TSC2tuberous sclerosis 2

References

  1. Ortman, J.M.; Velkoff, V.A.; Hogan, H. An Aging Nation: The Older Population in the United States; U.S. Census Bureau, Ed.; U.S. Census Bureau: Washington, DC, USA, 2014.
  2. Goisser, S.; Kemmler, W.; Porzel, S.; Volkert, D.; Sieber, C.C.; Bollheimer, L.C.; Freiberger, E. Sarcopenic obesity and complex interventions with nutrition and exercise in community-dwelling older persons—A narrative review. Clin. Interv. Aging 2015, 10, 1267–1282. [Google Scholar] [PubMed]
  3. Wolfe, R.R. The role of dietary protein in optimizing muscle mass, function and health outcomes in older individuals. Br. J. Nutr. 2012, 108, 88–93. [Google Scholar] [CrossRef] [PubMed]
  4. Arthur, S.T.; Cooley, I.D. The effect of physiological stimuli on sarcopenia; Impact of notch and Wnt signaling on impaired aged skeletal muscle repair. Int. J. Biol. Sci. 2012, 8, 731–760. [Google Scholar] [CrossRef] [PubMed]
  5. Centers for Disease Control and Prevention (CDC). The State of Aging and Health in America 2013; Centers for Disease Control and Prevention, Ed.; US Department of Health and Human Services: Atlanta, GA, USA, 2013.
  6. Chumlea, W.C.; Baumgartner, R.N.; Vellas, B.P. Anthropometry and body composition in the perspective of nutritional status in the elderly. Nutrition 1991, 7, 57–60. [Google Scholar] [PubMed]
  7. Baum, J.I.; Wolfe, R.R. The link between dietary protein intake, skeletal muscle function and health in older adults. Healthcare 2015, 3, 529–543. [Google Scholar] [CrossRef]
  8. Wolfe, R.R.; Miller, S.L.; Miller, K.B. Optimal protein intake in the elderly. Clin. Nutr. 2008, 27, 675–684. [Google Scholar] [CrossRef] [PubMed]
  9. Morais, J.A.; Chevalier, S.; Gougeon, R. Protein turnover and requirements in the healthy and frail elderly. J. Nutr. Health Aging 2006, 10, 272–283. [Google Scholar] [PubMed]
  10. Wilson, M.M.; Purushothaman, R.; Morley, J.E. Effect of liquid dietary supplements on energy intake in the elderly. Am. J. Clin. Nutr. 2002, 75, 944–947. [Google Scholar] [PubMed]
  11. Hu, F.B.; Stampfer, M.J.; Manson, J.E.; Rimm, E.; Colditz, G.A.; Speizer, F.E.; Hennekens, C.H.; Willett, W.C. Dietary protein and risk of ischemic heart disease in women. Am. J. Clin. Nutr. 1999, 70, 221–227. [Google Scholar] [PubMed]
  12. Obarzanek, E.; Velletri, P.A.; Cutler, J.A. Dietary protein and blood pressure. JAMA 1996, 275, 1598–1603. [Google Scholar] [CrossRef] [PubMed]
  13. Stamler, J.; Elliott, P.; Kesteloot, H.; Nichols, R.; Claeys, G.; Dyer, A.R.; Stamler, R. Inverse relation of dietary protein markers with blood pressure. Findings for 10,020 men and women in the INTERSALT Study. Circulation 1996, 94, 1629–1634. [Google Scholar] [CrossRef] [PubMed]
  14. Trumbo, P.; Schlicker, S.; Yates, A.A.; Poos, M. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein and amino acids. J. Am. Diet. Assoc. 2002, 102, 1621–1630. [Google Scholar] [CrossRef]
  15. Volpi, E.; Campbell, W.W.; Dwyer, J.T.; Johnson, M.A.; Jensen, G.L.; Morley, J.E.; Wolfe, R.R. Is the optimal level of protein intake for older adults greater than the recommended dietary allowance? J. Gerontol. 2013, 68, 677–681. [Google Scholar] [CrossRef] [PubMed]
  16. Fulgoni, V.L., III. Current protein intake in America: Analysis of the National Health and Nutrition Examination Survey, 2003–2004. Am. J. Clin. Nutr. 2008, 87, 1554–1557. [Google Scholar]
  17. Pasiakos, S.M.; Agarwal, S.; Lieberman, H.R.; Fulgoni, V.L., III. Sources and amounts of animal, dairy, and plant protein intake of US adults in 2007–2010. Nutrients 2015, 7, 7058–7069. [Google Scholar] [CrossRef] [PubMed]
  18. Katsanos, C.S.; Kobayashi, H.; Sheffield-Moore, M.; Aarsland, A.; Wolfe, R.R. A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly. Am. J. Physiol. Endocrinol. Metab. 2006, 291, 381–387. [Google Scholar] [CrossRef] [PubMed]
  19. Moore, D.R.; Churchward-Venne, T.A.; Witard, O.; Breen, L.; Burd, N.A.; Tipton, K.D.; Phillips, S.M. Protein ingestion to stimulate myofibrillar protein synthesis requires greater relative protein intakes in healthy older versus younger men. J. Gerontol. A 2015, 70, 57–62. [Google Scholar] [CrossRef] [PubMed]
  20. Wolfe, R.R. Regulation of muscle protein by amino acids. J. Nutr. 2002, 132, 3219–3224. [Google Scholar]
  21. Rasmussen, B.B.; Wolfe, R.R.; Volpi, E. Oral and intravenously administered amino acids produce similar effects on muscle protein synthesis in the elderly. J. Nutr. Health Aging 2002, 6, 358–362. [Google Scholar] [PubMed]
  22. Cuthbertson, D.; Smith, K.; Babraj, J.; Leese, G.; Waddell, T.; Atherton, P.; Wackerhage, H.; Taylor, P.M.; Rennie, M.J. Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle. FASEB J. 2005, 19, 422–424. [Google Scholar] [CrossRef] [PubMed]
  23. Symons, T.B.; Sheffield-Moore, M.; Wolfe, R.R.; Paddon-Jones, D. A moderate serving of high-quality protein maximally stimulates skeletal muscle protein synthesis in young and elderly subjects. J. Am. Diet. Assoc. 2009, 109, 1582–1586. [Google Scholar] [CrossRef] [PubMed]
  24. Anthony, J.C.; Anthony, T.G.; Kimball, S.R.; Vary, T.C.; Jefferson, L.S. Orally administered leucine stimulates protein synthesis in skeletal muscle of postabsorptive rats in association with increased eIF4F formation. J. Nutr. 2000, 130, 139–145. [Google Scholar] [PubMed]
  25. Anthony, J.C.; Yoshizawa, F.; Anthony, T.G.; Vary, T.C.; Jefferson, L.S.; Kimball, S.R. Leucine stimulates translation initiation in skeletal muscle of postabsorptive rats via a rapamycin-sensitive pathway. J. Nutr. 2000, 130, 2413–2419. [Google Scholar] [PubMed]
  26. Gordon, B.S.; Kelleher, A.R.; Kimball, S.R. Regulation of muscle protein synthesis and the effects of catabolic states. Int. J. Biochem. Cell Biol. 2013, 45, 2147–2157. [Google Scholar] [CrossRef] [PubMed]
  27. Børsheim, E.; Tipton, K.D.; Wolf, S.E.; Wolfe, R.R. Essential amino acids and muscle protein recovery from resistance exercise. Am. J. Physiol. Endocrinol. Metab. 2002, 283, 648–657. [Google Scholar] [CrossRef] [PubMed]
  28. Mitchell, C.J.; Gatta, P.A.D.; Petersen, A.C.; Cameron-Smith, D.; Markworth, J.F. Soy protein ingestion results in less prolonged p70S6 kinase phosphorylation compared to whey protein after resistance exercise in older men. J. Int. Soc. Sports Nutr. 2015, 12, 6. [Google Scholar] [CrossRef] [PubMed]
  29. Phillips, S.M.; Tang, J.E.; Moore, D.R. The role of milk- and soy-based protein in support of muscle protein synthesis and muscle protein accretion in young and elderly persons. J. Am. Coll. Nutr. 2009, 28, 343–354. [Google Scholar] [CrossRef] [PubMed]
  30. Tang, J.E.; Moore, D.R.; Kujbida, G.W.; Tarnopolsky, M.A.; Phillips, S.M. Ingestion of whey hydrolysate, casein, or soy protein isolate: Effects on mixed muscle protein synthesis at rest and following resistance exercise in young men. J. Appl. Physiol. 2009, 107, 987–992. [Google Scholar] [CrossRef] [PubMed]
  31. Houston, D.K.; Nicklas, B.J.; Ding, J.; Harris, T.B.; Tylavsky, F.A.; Newman, A.B.; Lee, J.S.; Sahyoun, N.R.; Visser, M.; Kritchevsky, S.B. Dietary protein intake is associated with lean mass change in older, community-dwelling adults: The Health, Aging, and Body Composition (Health ABC) Study. Am. J. Clin. Nutr. 2008, 87, 150–155. [Google Scholar] [PubMed]
  32. Montero-Fernandez, N.; Serra-Rexach, J.A. Role of exercise on sarcopenia in the elderly. Eur. J. Phys. Rehabil. Med. 2013, 49, 131–143. [Google Scholar] [PubMed]
  33. Nowson, C.; O‘Connell, S. Protein requirements and recommendations for older people: A review. Nutrients 2015, 7, 6874–6899. [Google Scholar] [CrossRef] [PubMed]
  34. Keys, A.; Taylor, H.L.; Grande, F. Basal metabolism and age of adult man. Metabolism 1973, 22, 579–587. [Google Scholar] [CrossRef]
  35. Roberts, S.B.; Dallal, G.E. Energy requirements and aging. Public Health Nutr. 2005, 8, 1028–1036. [Google Scholar] [CrossRef] [PubMed]
  36. Pencharz, P.B.; Elango, R.; Wolfe, R.R. Recent developments in understanding protein needs—How much and what kind should we eat? Appl. Physiol. Nutr. Metab. 2016, 41, 577–580. [Google Scholar] [CrossRef] [PubMed]
  37. Brown, C.J.; Flood, K.L. Mobility limitation in the older patient: A clinical review. JAMA 2013, 310, 1168–1177. [Google Scholar] [CrossRef] [PubMed]
  38. Wolfe, R.R. The underappreciated role of muscle in health and disease. Am. J. Clin. Nutr. 2006, 84, 475–482. [Google Scholar] [PubMed]
  39. Phillips, S.M.; Fulgoni, V.L., III; Heaney, R.P.; Nicklas, T.A.; Slavin, J.L.; Weaver, C.M. Commonly consumed protein foods contribute to nutrient intake, diet quality, and nutrient adequacy. Am. J. Clin. Nutr. 2015, 106, 1346–1352. [Google Scholar] [CrossRef] [PubMed]
  40. Paddon-Jones, D.; Rasmussen, B.B. Dietary protein recommendations and the prevention of sarcopenia. Curr. Opin. Clin. Nutr. Metab. Care 2009, 12, 86–90. [Google Scholar] [CrossRef] [PubMed]
  41. Nair, K.S.; Garrow, J.S.; Ford, C.; Mahler, R.F.; Halliday, D. Effect of poor diabetic control and obesity on whole body protein metabolism in man. Diabetologia 1983, 25, 400–403. [Google Scholar] [CrossRef] [PubMed]
  42. Deutz, N.E.; Ten Have, G.A.M.; Soeters, P.B.; Moughan, P.J. Increased intestinal amino-acid retention from the addition of carbohydrates to a meal. Clin. Nutr. 1995, 14, 354–364. [Google Scholar] [CrossRef]
  43. Volpi, E.; Mittendorfer, B.; Wolf, S.E.; Wolfe, R.R. Oral amino acids stimulate muscle protein anabolism in the elderly despite higher first-pass splanchnic extraction. Am. J. Physiol. 1999, 277, 513–520. [Google Scholar]
  44. Kim, I.-Y.; Schutzler, S.; Schrader, A.; Spencer, H.J.; Azhar, G.; Ferrando, A.A.; Wolfe, R.R. The anabolic response to a meal containing different amounts of protein is not limited by the maximal stimulation of protein synthesis in healthy young adults. Am. J. Physiol. Endocrinol. Metab. 2016, 310, 73–80. [Google Scholar] [CrossRef] [PubMed]
  45. Kim, I.-Y.; Schutzler, S.; Schrader, A.; Spencer, H.; Kortebein, P.; Deutz, N.E.P.; Wolfe, R.R.; Ferrando, A.A. Quantity of dietary protein intake, but not pattern of intake, affects net protein balance primarily through differences in protein synthesis in older adults. Am. J. Physiol. Endocrinol. Metab. 2015, 308, 21–28. [Google Scholar] [CrossRef] [PubMed]
  46. Engelen, M.P.K.J.; Com, G.; Wolfe, R.R.; Deutza, N.E.P. Dietary essential amino acids are highly anabolic in pediatric patients with cystic fibrosis. J. Cyst. Fibros. 2013, 12, 445–453. [Google Scholar] [CrossRef] [PubMed]
  47. Jonker, R.; Deutz, N.E.P.; Erbland, M.L.; Anderson, P.J.; Engelen, M.P.K.J. Hydrolyzed casein and whey protein meals comparably stimulate net whole-body protein synthesis in COPD patients with nutritional depletion without an additional effect of leucine co-ingestion. Clin. Nutr. 2014, 33, 211–220. [Google Scholar] [CrossRef] [PubMed]
  48. Hegsted, D.M. Assessment of nitrogen requirements. Am. J. Clin. Nutr. 1978, 31, 1669–1677. [Google Scholar] [PubMed]
  49. Sakuma, K.; Aoi, W.; Yamaguchi, A. Current understanding of sarcopenia: Possible candidates modulating muscle mass. Pflugers Arch. 2015, 467, 213–229. [Google Scholar] [CrossRef] [PubMed]
  50. Sakuma, K.; Aoi, W.; Yamaguchi, A. The intriguing regulators of muscle mass in sarcopenia and muscular dystrophy. Front. Aging Neurosci. 2014, 6, 230. [Google Scholar] [CrossRef] [PubMed]
  51. Kimball, S.R.; Jefferson, L.S. Signaling pathways and molecular mechanisms through which branched-chain amino acids mediate translational control of protein synthesis. J. Nutr. 2006, 136 (Suppl. 1), 227–231. [Google Scholar]
  52. Kimball, S.R.; Jefferson, L.S. Role of amino acids in the translational control of protein synthesis in mammals. Semin. Cell Dev. Biol. 2005, 16, 21–27. [Google Scholar] [CrossRef] [PubMed]
  53. Kimball, S.R.; Jefferson, L.S. Regulation of global and specific mRNA translation by oral administration of branched-chain amino acids. Biochem. Biophys. Res. Commun. 2004, 313, 423–427. [Google Scholar] [CrossRef] [PubMed]
  54. Wilkinson, D.J.; Hossain, T.; Hill, D.S.; Phillips, B.E.; Crossland, H.; Williams, J.; Loughna, P.; Churchward-Venne, T.A.; Breen, L.; Phillips, S.M.; et al. Effects of leucine and its metabolite β-hydroxy-β-methylbutyrate on human skeletal muscle protein metabolism. J. Physiol. 2013, 591, 2911–2923. [Google Scholar] [CrossRef] [PubMed]
  55. Katsanos, C.S.; Kobayashi, H.; Sheffield-Moore, M.; Aarsland, A.; Wolfe, R.R. Aging is associated with diminished accretion of muscle proteins after the ingestion of a small bolus of essential amino acids. Am. J. Clin. Nutr. 2005, 82, 1065–1073. [Google Scholar] [PubMed]
  56. Paddon-Jones, D.; Sheffield-Moore, M.; Creson, D.L.; Sanford, A.P.; Wolf, S.E.; Wolfe, R.R.; Ferrando, A.A. Hypercortisolemia alters muscle protein anabolism following ingestion of essential amino acids. Am. J. Physiol. Endocrinol. Metab. 2003, 284, 946–953. [Google Scholar] [CrossRef] [PubMed]
  57. Guillet, C.; Prod’Homme, M.; Balage, M.; Gachon, P.; Giraudet, C.; Morin, L.; Grizard, J.; Boirie, Y. Impaired anabolic response of muscle protein synthesis is associated with S6K1 dysregulation in elderly humans. FASEB J. 2004, 18, 1586–1587. [Google Scholar] [CrossRef] [PubMed]
  58. Fry, C.S.; Drummond, M.J.; Glynn, E.L.; Dickinson, J.M.; Gundermann, D.M.; Timmerman, K.L.; Walker, D.K.; Volpi, E.; Rasmussen, B.B. Skeletal muscle autophagy and protein breakdown following resistance exercise are similar in younger and older adults. J. Gerontol. A 2013, 68, 599–607. [Google Scholar] [CrossRef] [PubMed]
  59. Drummond, M.J.; Miyazaki, M.; Dreyer, H.C.; Pennings, B.; Dhanani, S.; Volpi, E.; Esser, K.A.; Rasmussen, B.B. Expression of growth-related genes in young and older human skeletal muscle following an acute stimulation of protein synthesis. J. Appl. Physiol. 2009, 106, 1403–1411. [Google Scholar] [CrossRef] [PubMed]
  60. Drummond, M.J.; Dickinson, J.M.; Fry, C.S.; Walker, D.K.; Gundermann, D.M.; Reidy, P.T.; Timmerman, K.L.; Markofski, M.M.; Paddon-Jones, D.; Rasmussen, B.B.; et al. Bed rest impairs skeletal muscle amino acid transporter expression, mTORC1 signaling, and protein synthesis in response to essential amino acids in older adults. Am. J. Physiol. Endocrinol. Metab. 2012, 302, 1113–1122. [Google Scholar] [CrossRef] [PubMed]
  61. Tanner, R.E.; Brunker, L.B.; Agergaard, J.; Barrows, K.M.; Briggs, R.A.; Kwon, O.S.; Young, L.M.; Hopkins, P.N.; Volpi, E.; Marcus, R.L. Age-related differences in lean mass, protein synthesis and skeletal muscle markers of proteolysis after bed rest and exercise rehabilitation. J. Physiol. 2015, 593, 4259–4273. [Google Scholar] [CrossRef] [PubMed]
  62. Bukhari, S.S.; Phillips, B.E.; Wilkinson, D.J.; Limb, M.C.; Rankin, D.; Mitchell, W.K.; Kobayashi, H.; Greenhaff, P.L.; Smith, K.; Atherton, P.J. Intake of low-dose leucine-rich essential amino acids stimulates muscle anabolism equivalently to bolus whey protein in older women at rest and after exercise. Am. J. Physiol. Endocrinol. Metab. 2015, 308, 1056–1065. [Google Scholar] [CrossRef] [PubMed]
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