Ginseng Berry Juice (GBJ) Regulates the Inflammation in Acute Ulcerative Mouse Models and the Major Bioactive Substances Are Ginsenosides Rb3, Rc, Rd, and Re
Abstract
1. Introduction
2. Materials and Methods
2.1. Plant Materials and Analytic Conditions
2.2. Analysis of Biomarkers
2.3. HCl/Ethanol-Induced Acute Gastric Injury Model
2.4. Macroscopic and Microscopic Analysis
2.5. Immunofluorescent and Immunohistochemical Analyses
2.6. Ethics Statement
2.7. Statistical Analysis
3. Results
3.1. GBJ Prevented the HCl/Ethanol-Induced Morphological Changes
3.2. BGJ Prevented the HCl/Ethanol-Induced Histopathological Changes
3.3. GBJ Suppressed the Expression of Pro-Inflammatory Cytokines Such as TNF-α, IL-6, and IL-13 but Increased the Expression of Anti-Inflammatory Cytokines Such as IL-10
3.4. GBJ Controls the Translocation of NF-κBp65 from the Cytoplasm to the Nucleus to Prevent Inflammation and Then Suppresses the Production of Cyclooxygenase 2 (COX-2) and Prostaglandin E2
3.5. Preparation and Quantitative Analysis of Anti-Ulcerative Biomarkers in GBJ
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Time | % A | % B | |
---|---|---|---|
Conditions | 0 | 10 | 90 |
3 | 12 | 88 | |
6 | 14 | 86 | |
9 | 16 | 84 | |
12 | 18 | 82 | |
32 | 26 | 74 | |
40 | 36 | 64 | |
50 | 50 | 50 | |
55 | 100 | 0 | |
57 | 10 | 90 | |
60 | 10 | 90 |
Biomarker | Content (%) |
---|---|
Ginsenoside rb3 | 2.55 ± 0.04 |
Ginsenoside re | 5.21 ± 0.21 |
Ginsenoside rc | 2.12 ± 0.16 |
Ginsenoside rd | 2.73 ± 0.02 |
Chlorogenic acid | 0.16 ± 0.03 |
Caffeic acid | 0.07 ± 0.001 |
p-Coumaric acid | 0.03 ± 0.001 |
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Lee, S.-Y.; Song, S.-Y.; Lee, S.-H.; Kim, G.-Y.; Park, J.-W.; Bae, C.-S.; Park, D.-H.; Cho, S.-S. Ginseng Berry Juice (GBJ) Regulates the Inflammation in Acute Ulcerative Mouse Models and the Major Bioactive Substances Are Ginsenosides Rb3, Rc, Rd, and Re. Nutrients 2024, 16, 1031. https://doi.org/10.3390/nu16071031
Lee S-Y, Song S-Y, Lee S-H, Kim G-Y, Park J-W, Bae C-S, Park D-H, Cho S-S. Ginseng Berry Juice (GBJ) Regulates the Inflammation in Acute Ulcerative Mouse Models and the Major Bioactive Substances Are Ginsenosides Rb3, Rc, Rd, and Re. Nutrients. 2024; 16(7):1031. https://doi.org/10.3390/nu16071031
Chicago/Turabian StyleLee, Soon-Young, Seung-Yub Song, Sung-Ho Lee, Gye-Yeop Kim, Jin-Woo Park, Chun-Sik Bae, Dae-Hun Park, and Seung-Sik Cho. 2024. "Ginseng Berry Juice (GBJ) Regulates the Inflammation in Acute Ulcerative Mouse Models and the Major Bioactive Substances Are Ginsenosides Rb3, Rc, Rd, and Re" Nutrients 16, no. 7: 1031. https://doi.org/10.3390/nu16071031
APA StyleLee, S.-Y., Song, S.-Y., Lee, S.-H., Kim, G.-Y., Park, J.-W., Bae, C.-S., Park, D.-H., & Cho, S.-S. (2024). Ginseng Berry Juice (GBJ) Regulates the Inflammation in Acute Ulcerative Mouse Models and the Major Bioactive Substances Are Ginsenosides Rb3, Rc, Rd, and Re. Nutrients, 16(7), 1031. https://doi.org/10.3390/nu16071031