Palmitic Acid Modulates Microglial Cell Response to Metabolic Endotoxemia in an In Vitro Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals Used in the Study
2.2. Microglial Cell Culture
2.3. Palmitic Acid Preparation
2.4. Microglial Cell Viability and Morphology
2.5. Conditioned Media
2.6. Oxidative Stress Assessment
2.7. IL-6 and MCP-1 Assays
2.8. Statistical Analysis
3. Results
3.1. HMC3 Viability and Morphology
3.2. Palmitic Acid and Endotoxin Modulate Cytokine Secretion by Microglial Cells
3.3. Palmitic Acid and Endotoxin Induce Oxidative Stress in Microglial Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chmielarz, M.; Sobieszczańska, B.; Teisseyre, A.; Wawrzyńska, M.; Bożemska, E.; Środa-Pomianek, K. Palmitic Acid Modulates Microglial Cell Response to Metabolic Endotoxemia in an In Vitro Study. Nutrients 2023, 15, 3463. https://doi.org/10.3390/nu15153463
Chmielarz M, Sobieszczańska B, Teisseyre A, Wawrzyńska M, Bożemska E, Środa-Pomianek K. Palmitic Acid Modulates Microglial Cell Response to Metabolic Endotoxemia in an In Vitro Study. Nutrients. 2023; 15(15):3463. https://doi.org/10.3390/nu15153463
Chicago/Turabian StyleChmielarz, Mateusz, Beata Sobieszczańska, Andrzej Teisseyre, Magdalena Wawrzyńska, Edyta Bożemska, and Kamila Środa-Pomianek. 2023. "Palmitic Acid Modulates Microglial Cell Response to Metabolic Endotoxemia in an In Vitro Study" Nutrients 15, no. 15: 3463. https://doi.org/10.3390/nu15153463