Compliance with Nutritional Recommendations and Gut Microbiota Profile in Galician Overweight/Obese and Normal-Weight Individuals
Abstract
:1. Introduction
2. Materials and Methods
2.1. Population and Sample Size
2.2. Anthropometric Measures
2.3. Dietary Information
2.4. Fecal Sample Collection and DNA Extraction
2.5. 16S rRNA Amplicon Sequencing
2.6. Statistical and Bioinformatic Analysis
3. Results
3.1. Nutritional Analysis and Adequation to the Objectives and the Recommended Daily Intakes for the Spanish Population
3.2. Analysis of the Gut Microbiota Composition
3.2.1. Alpha- and Beta-Diversity
3.2.2. Relative Abundance of Bacteria
3.2.3. Correlation Analysis: Microbiota-Fiber, Microbiota-MUFAs
3.2.4. LEfSe Analysis
4. Discussion
4.1. Nutritional Analysis
4.2. Analysis of the Gut Microbiota Composition
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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DIET ADEQUACY | |||||
---|---|---|---|---|---|
CALORIC PROFILE | NUTRITIONAL OBJECTIVES | VALUES OF PARTICIPANTS’ DIET | DÉFICIT | ADEQUATE | EXCESS |
Carbohydrates (% Energy) | 50–55 | 38.9 ± 7.8 | 96 (88.1%) | 11 (10.1%) | 1 (0.9%) |
Fiber (g/1000 kcal) | >14 | 11.7 ± 7.7 | 91 (84.3%) | 15 (13.9%) | 2 (1.9%) |
Lipids (% Energy) | 30–35 | 41.8 ± 8.0 | 7 (6.4%) | 15 (13.9%) | 86 (79.6%) |
LIPID PROFILE | |||||
SFA (% Energy) | ≤7–8 | 12.0 ± 3.2 | - | 13 (0.0%) | 95 (88%) |
MUFA (% Energy) | 20 | 20.8 ± 16.1 | 36 (33.3%) | 45 (41.7%) | 27 (25.0%) |
PUFA (% Energy) | 5 | 5.9 ± 3.6 | 39 (38.0%) | 23 (21.2%) | 62 (57.4%) |
Cholesterol (mg) | <300 | 347.0 ± 138.6 | 0 (0.0%) | 46 (42.6%) | 62 (57.4%) |
OTHERS | |||||
Water (mL) | 2000 | 2063.3 ± 627.9 | 49 (45.4%) | 59 (54.6%) | 0 (0.0%) |
Fruits and vegetables | 5 portions | 1.8 ± 1.5 | 102 (94.4%) | 5 (4.6%) | 1 (0.9%) |
Sugar (% Energy) | 6–10 | 18.1 ± 7.4 | - | 13 (12.0%) | 95 (88.0%) |
Alcohol (g) | ≤1 SDU women ≤2 SDU men | 10.84 ± 44.43 | - | 100 (92.6%) | 8 (7.4%) |
NW (n = 40) | OW (n = 35) | OB (n = 33) | ||||||
---|---|---|---|---|---|---|---|---|
CALORIC PROFILE | Nutritional Objectives | Mean ± SD | Intake Adequacy: n (%) | Mean ± SD | Intake Adequacy: n (%) | Mean ± SD | Intake Adequacy: n (%) | p Value |
Carbohydrates (% Energy) | 50–55% | 40.3 ± 7.1 | 5 (12.5%) | 37.4 ± 7.7 | 1 (3.0%) | 38.8 ± 8.6 | 4(11.4%) | 0.274 |
Lipids (% Energy) | 30–35% | 40.6 ± 6.3 | 6 (15.0%) | 43.6 ± 9.6 | 5 (15.2%) | 41.9 ± 7.5 | 5 (14.3%) | 0.280 |
Fiber (g/1000 Kcal) | >14 | 14.2 ± 9.5 | 13 (32.5%) | 12.5 ± 10.14 | 4 (12.0%) | 9.1 ± 2.9 | 5 (14.3%) | 0.038 |
LIPID PROFILE | ||||||||
SFA (% Energy) | ≤7–8 | 11.8 ± 2.8 | 3 (7.5%) | 11.9 ± 3.0 | 4 (12.1%) | 15.5 ± 8.1 | 0 (0.0%) | 0.559 |
MUFA (% Energy) | 20 | 20.3 ± 5.7 | 10 (25.0%) | 19.4 ± 5.7 | 12 (36.4%) | 6.5 ± 5.3 | 5 (14.3%) | <0.001 |
PUFA (% Energy) | 5 | 5.8 ± 2.1 | 12 (30.0%) | 6.5 ± 5.3 | 19 (57.6%) | 5.4 ± 2.7 | 6 (17.4%) | 0.874 |
Cholesterol (mg) | <300 mg | 340.3 ± 137.7 | 19 (47.5%) | 348.0 ± 129.8 | 11 (33.0%) | 354.1 ± 152.1 | 10 (28.6%) | 0.915 |
OTHERS | ||||||||
Water (mL) | 2000 mL | 2305.36 ± 487.6 | 23 (57.5%) | 2128.0 ± 682.2 | 20 (57.4%) | 2098.1 ± 736.4 | 16 (48.5%) | 0.751 |
Fruits and vegetables | 5 portions | 1.9 ± 1.5 | 3 (7.5%) | 1.3 ± 1.4 | 1 (2.8%) | 2.2 ± 1.5 | 1 (3.0%) | 0.785 |
Sugar | 6–10 | 20.0 ± 9.0 | 1 (2.5%) | 19.0 ± 7.1 | 5 (14.3%) | 16.0 ± 5.1 | 7 (21.2%) | 0.632 |
Alcohol (g) | ≤1 SDU women ≤2 SDU men | 3.8 ± 9.8 | 39 (97.5%) | 4.0 ± 8.7 | 32 (97.0%) | 3.9 ± 8.8 | 31 (88.6%) | 0.298 |
NW (n = 40) | OW (n = 35) | OB (n = 33) | |||||
---|---|---|---|---|---|---|---|
Vitamins | Average ± SD | Intake Adequacy n (%) | Average ± SD | Intake Adequacy n (%) | Average ± SD | Intake Adequacy n (%) | p Value |
Thiamine (mg) | 1.5 ± 0.8 | 32 (80.0%) | 1.9 ± 0.9 | 31 (88.6%) | 1.4 ± 0.3 | 29 (87.9%) | 0.009 |
Riboflavin (mg) | 1.7 ± 0.5 | 30 (75.0%) | 2.1 ± 0.6 | 31 (88.6%) | 1.7 ± 0.4 | 27 (81.9%) | 0.007 |
Niacin (mg) | 30.5 ± 10.7 | 30 (75.0%) | 40.4 ±14.5 | 16 (45.7%) | 31.6 ± 7.7 | 25 (75.8%) | 0.0005 |
Vitamin B6 (µg) | 2.0 ± 0.8 | 35 (87.5%) | 2.3 ± 1.1 | 33 (94.3%) | 2.0 ± 0.6 | 30 (90.9%) | 0.159 |
Folic acid (µg) | 281.3 ± 126.3 | 13 (32.5%) | 278.3 ± 105.2 | 10 (28.6%) | 255.3 ± 74.6 | 8 (24.2%) | 0.537 |
Vitamin B12 (µg) | 6.1 ± 6.0 | 40 (100%) | 6.4 ± 2.5 | 35 (100%) | 5.6 ± 2.8 | 33 (100%) | 0.697 |
Vitamin C (mg) | 151.2 ± 87.7 | 35 (87.5%) | 166.2 ± 79.6 | 33 (94.3%) | 160.7 ± 68.5 | 33 (100%) | 0.738 |
Vitamin A (µg) | 798.4 ± 603.1 | 24 (60.0%) | 750.5 ± 452.7 | 19 (54.3%) | 745.0 ± 406.8 | 17 (51.5%) | 0.868 |
Vitamin D (µg) | 2.9 ± 3.2 | 8 (20.0%) | 4.6 ± 6.0 | 11 (31.4%) | 4.5 ± 6.5 | 11 (33.3%) | 0.321 |
Vitamin E (mg) | 8.3 ± 8.1 | 5 (12.5%) | 7.8 ± 9.9 | 4 (11.4%) | 5.9 ± 5.6 | 2 (6.1%) | 0.416 |
Minerals | |||||||
Calcium (mg) | 719.8 ± 297.1 | 7 (17.5%) | 841.2 ± 415.0 | 12 (34.3%) | 792.9 ± 348.4 | 10 (30.3%) | 0.331 |
Magnesium (mg) | 325.7 ± 134.9 | 23 (57.5%) | 368.9 ± 95.8 | 23 (65.7%) | 308.8 ± 81.0 | 15 (45.5%) | 0.063 |
Potassium (mg) | 3086.7 ± 904.1 | 22 (55.0%) | 4044.5 ± 1135.9 | 26 (74.3%) | 3511.8 ± 745.6 | 24 (72.7%) | 0.0001 |
Phosporus (mg) | 1415.5 ± 500.5 | 38 (95.0%) | 1716.9 ± 472.9 | 34 (97.1%) | 1436.8 ± 380.6 | 32 (97.0%) | 0.009 |
Iron (mg) | 16.7 ± 9.0 | 23 (57.5%) | 18.3 ± 5.8 | 29 (82.9%) | 14.0 ± 2.8 | 21 (63.6%) | 0.035 |
Iodine (µg) | 184.4 ± 150.3 | 16 (40.0%) | 347.5 ± 197.4 | 25 (71.4%) | 292.5 ± 153.6 | 25 (75.8%) | 0.0002 |
Zinc (mg) | 24.4 ± 78.7 | 29 (72.5%) | 14.1 ± 3.5 | 34 (97.1%) | 10.8 ± 2.9 | 27 (81.8%) | 0.0445 |
Sodium (mg) | 2610.9 ± 677.5 | 6 (15.0%) | 5609.5 ± 1219.7 | 0 (0.0%) | 3877.1 ± 346.0 | 0 (0.0%) | <0.0001 |
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Sinisterra-Loaiza, L.; Alonso-Lovera, P.; Cardelle-Cobas, A.; Miranda, J.M.; Vázquez, B.I.; Cepeda, A. Compliance with Nutritional Recommendations and Gut Microbiota Profile in Galician Overweight/Obese and Normal-Weight Individuals. Nutrients 2023, 15, 3418. https://doi.org/10.3390/nu15153418
Sinisterra-Loaiza L, Alonso-Lovera P, Cardelle-Cobas A, Miranda JM, Vázquez BI, Cepeda A. Compliance with Nutritional Recommendations and Gut Microbiota Profile in Galician Overweight/Obese and Normal-Weight Individuals. Nutrients. 2023; 15(15):3418. https://doi.org/10.3390/nu15153418
Chicago/Turabian StyleSinisterra-Loaiza, Laura, Patricia Alonso-Lovera, Alejandra Cardelle-Cobas, Jose Manuel Miranda, Beatriz I. Vázquez, and Alberto Cepeda. 2023. "Compliance with Nutritional Recommendations and Gut Microbiota Profile in Galician Overweight/Obese and Normal-Weight Individuals" Nutrients 15, no. 15: 3418. https://doi.org/10.3390/nu15153418