Peonidin-3-O-glucoside and Resveratrol Increase the Viability of Cultured Human hFOB Osteoblasts and Alter the Expression of Genes Associated with Apoptosis, Osteoblast Differentiation and Osteoclastogenesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Culture and Treatment of hFOB Osteoblasts
2.2. mRNAseq Library Preparation, Validation, Quantification and qPCR
2.3. Bioinformatics, Statistics and Database Annotation
2.4. Ingenuity® Pathway Analysis (IPA)
2.5. Data Availability and Gene Expression Omnibus (GEO) Deposition
3. Results
3.1. Serum Starvation Reduces Growth and Induces Apoptosis in Cultured hFOB Osteoblasts
3.2. Transcriptomic and IPA Analysis of Osteoblast Apoptosis
3.3. Transcriptomic Analysis of Treated Human hFOB Osteoblasts
3.4. Analysis of Osteoblastogenesis, Rankl and HIF Signaling Networks
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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A | B | ||
Geneid | Gene name | Geneid | Gene name |
ENSG00000120915 | EPHX2 | ENSG00000282608 | ADORA3 |
ENSG00000267677 | RP11-27G24.1 | ENSG00000213761 | MT1P1 |
ENSG00000273313 | RBAKDN | ENSG00000283045 | RP11-764D10.2 |
ENSG00000259215 | RP11-253M7.4 | ENSG00000227517 | LINC01483 |
ENSG00000251600 | RP11-673E1.1 | ENSG00000169442 | CD52 |
ENSG00000268750 | CTD-2583A14.10 | ENSG00000274421 | RP11-386J22.3 |
ENSG00000159496 | RGL4 | ENSG00000280639 | LINC02204 |
ENSG00000110328 | GALNT18 | ENSG00000267938 | EIF1P6 |
ENSG00000260331 | RP11-111J6.2 | ENSG00000198844 | ARHGEF15 |
ENSG00000233087 | WTH3DI | ENSG00000261783 | RP11-252K23.2 |
C | D | ||
Geneid | Gene name | Geneid | Gene name |
ENSG00000196415 | PRTN3 | ENSG00000159239 | C2orf81 |
ENSG00000120915 | EPHX2 | ENSG00000118017 | A4GNT |
ENSG00000227076 | RP11-4C20.4 | ENSG00000241112 | RPL29P14 |
ENSG00000183484 | GPR132 | ENSG00000109424 | UCP1 |
ENSG00000173930 | SLCO4C1 | ENSG00000261832 | RP11-435I10.4 |
ENSG00000260947 | RP11-384P7.7 | ENSG00000144868 | TMEM108 |
ENSG00000149633 | KIAA1755 | ENSG00000236670 | KRT18P5 |
ENSG00000137843 | PAK6 | ENSG00000235651 | AC064850.4 |
ENSG00000144488 | ESPNL | ENSG00000205502 | C2CD4B |
ENSG00000183807 | FAM162B | ENSG00000198535 | C2CD4A |
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Ostos Mendoza, K.C.; Garay Buenrostro, K.D.; Kanabar, P.N.; Maienschein-Cline, M.; Los, N.S.; Arbieva, Z.; Raut, N.A.; Lawal, T.O.; López, A.M.; Cabada-Aguirre, P.; et al. Peonidin-3-O-glucoside and Resveratrol Increase the Viability of Cultured Human hFOB Osteoblasts and Alter the Expression of Genes Associated with Apoptosis, Osteoblast Differentiation and Osteoclastogenesis. Nutrients 2023, 15, 3233. https://doi.org/10.3390/nu15143233
Ostos Mendoza KC, Garay Buenrostro KD, Kanabar PN, Maienschein-Cline M, Los NS, Arbieva Z, Raut NA, Lawal TO, López AM, Cabada-Aguirre P, et al. Peonidin-3-O-glucoside and Resveratrol Increase the Viability of Cultured Human hFOB Osteoblasts and Alter the Expression of Genes Associated with Apoptosis, Osteoblast Differentiation and Osteoclastogenesis. Nutrients. 2023; 15(14):3233. https://doi.org/10.3390/nu15143233
Chicago/Turabian StyleOstos Mendoza, Keila C., Karen D. Garay Buenrostro, Pinal N. Kanabar, Mark Maienschein-Cline, Nina S. Los, Zarema Arbieva, Nishikant A. Raut, Temitope O. Lawal, Alice M. López, Paulina Cabada-Aguirre, and et al. 2023. "Peonidin-3-O-glucoside and Resveratrol Increase the Viability of Cultured Human hFOB Osteoblasts and Alter the Expression of Genes Associated with Apoptosis, Osteoblast Differentiation and Osteoclastogenesis" Nutrients 15, no. 14: 3233. https://doi.org/10.3390/nu15143233