The Gliadin Hydrolysis Capacity of B. longum, L. acidophilus, and L. plantarum and Their Protective Effects on Caco-2 Cells against Gliadin-Induced Inflammatory Responses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Probiotic Preparations
2.2. Sourdough Fermentation
2.3. Gliadin Extract
2.4. SDS-PAGE Electrophoresis of Gliadins
2.5. In Vitro Digestion of Gliadins
2.6. Cell Culture Conditions
2.7. Evaluating B. longum, L. acidophilous, and L. plantarum Effects on the Inflammatory Effects of Gliadin Peptides in Intestinal Epithelial (Caco-2) Cells
2.8. Sandwich Enzyme-Linked Immunosorbent Assay (ELISA)
2.9. RNA Extraction and Quantitative Real-Time PCR (qRT-PCR)
2.10. Statistical Analysis
2.11. Ethical Approval
3. Results
3.1. Gliadin Hydrolysis by Selected Probiotics
3.2. IL-6 and INF-γ Protein Expression
3.3. IL-6, IL-17A, IFN-γ, IL-10, and TGF-β mRNA Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ramedani, N.; Seidita, A.; Asri, N.; Azimirad, M.; Yadegar, A.; Jahani-Sherafat, S.; Sharifan, A.; Mansueto, P.; Carroccio, A.; Rostami-Nejad, M. The Gliadin Hydrolysis Capacity of B. longum, L. acidophilus, and L. plantarum and Their Protective Effects on Caco-2 Cells against Gliadin-Induced Inflammatory Responses. Nutrients 2023, 15, 2769. https://doi.org/10.3390/nu15122769
Ramedani N, Seidita A, Asri N, Azimirad M, Yadegar A, Jahani-Sherafat S, Sharifan A, Mansueto P, Carroccio A, Rostami-Nejad M. The Gliadin Hydrolysis Capacity of B. longum, L. acidophilus, and L. plantarum and Their Protective Effects on Caco-2 Cells against Gliadin-Induced Inflammatory Responses. Nutrients. 2023; 15(12):2769. https://doi.org/10.3390/nu15122769
Chicago/Turabian StyleRamedani, Najmeh, Aurelio Seidita, Nastaran Asri, Masoumeh Azimirad, Abbas Yadegar, Somayeh Jahani-Sherafat, Anousheh Sharifan, Pasquale Mansueto, Antonio Carroccio, and Mohammad Rostami-Nejad. 2023. "The Gliadin Hydrolysis Capacity of B. longum, L. acidophilus, and L. plantarum and Their Protective Effects on Caco-2 Cells against Gliadin-Induced Inflammatory Responses" Nutrients 15, no. 12: 2769. https://doi.org/10.3390/nu15122769
APA StyleRamedani, N., Seidita, A., Asri, N., Azimirad, M., Yadegar, A., Jahani-Sherafat, S., Sharifan, A., Mansueto, P., Carroccio, A., & Rostami-Nejad, M. (2023). The Gliadin Hydrolysis Capacity of B. longum, L. acidophilus, and L. plantarum and Their Protective Effects on Caco-2 Cells against Gliadin-Induced Inflammatory Responses. Nutrients, 15(12), 2769. https://doi.org/10.3390/nu15122769