The Protective Effects of Corn Oligopeptides on Acute Alcoholic Liver Disease by Inhibiting the Activation of Kupffer Cells NF-κB/AMPK Signal Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of CPs
2.2. Identification of Corn Oligopeptides
2.3. Animal Models
2.4. Evaluation of Hepatic Biomarkers
2.4.1. Enzyme Activities
2.4.2. ELISA for TNF-α, IL-1, and IL-6 in Mice Serum
2.4.3. Real-Time Quantitative PCR
2.4.4. Isolation and Culture of Murine Kupffer Cells (KCs)
2.4.5. Extraction of Proteins
2.5. Western Blot Analyses
2.6. Statistical Analyses
3. Results
3.1. The Chemical Composition of CPs
3.2. The Inhibition Effects of CPs on AST and ALT in Mice Serum
3.3. The Inhibition Effects of CPs on Inflammatory Factors in Mice Serum
3.4. The Molecular Mechanism of Effects from CPs on LPS-Induced Kuffer Cells (KCs)
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Sequences (5′-3′) | Product Length |
---|---|---|
TNF-α | forward: CATCTTCTCAAAATTCGAGTGACAA | 447 bp |
reverse: TGGGAGTAGACAAGGTACAACCC | ||
IL-1 | forward: CTTCATCTTTGAAGAAGAGCCC | 418 bp |
reverse: CTCTGCAGACTCAAACTCCAC | ||
IL-6 | forward: TTCACAAGTCCGGACAGGAG | 488 bp |
reverse: TGGTCTTGGTCCTTAGCCAC 3 | ||
GAPDH | forward: GAAGGTGAAGGTCGGAGTCA | 402 bp |
reverse: TTCACACCCATGACGAACAT |
Corn Oligopetides | |
---|---|
Moisture (%) | 3.78 ± 0.11 |
Ash (%) | 3.90 ± 0.13 |
Protein (%) | 83.60 ± 1.21 |
Peptide (%) | 79.23 ± 1.19 |
Amino acid composition (%) | |
Ala | 8.17 |
Pro | 6.52 |
Val | 2.72 |
Met | 1.97 |
Ile | 1.99 |
Leu | 16.73 |
Phe | 4.33 |
Trp | 0.23 |
Asp | 4.73 |
Ser | 4.14 |
Glu | 22.72 |
His | 1.03 |
Gly | 1.18 |
Arg | 1.35 |
Thr | 2.22 |
Cys | 2.17 |
Tyr | 4.29 |
Lys | 0.25 |
Molecular Weight (u) | Over 10,000 | 3000–10,000 | 1000–3000 | 150–1000 | Below 150 |
---|---|---|---|---|---|
Distributions (%) | 0.0000 | 0.1513 | 3.3431 | 77.5960 | 18.9096 |
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Wei, Y.; Li, M.; Feng, Z.; Zhang, D.; Sun, M.; Wang, Y.; Chen, X. The Protective Effects of Corn Oligopeptides on Acute Alcoholic Liver Disease by Inhibiting the Activation of Kupffer Cells NF-κB/AMPK Signal Pathway. Nutrients 2022, 14, 4194. https://doi.org/10.3390/nu14194194
Wei Y, Li M, Feng Z, Zhang D, Sun M, Wang Y, Chen X. The Protective Effects of Corn Oligopeptides on Acute Alcoholic Liver Disease by Inhibiting the Activation of Kupffer Cells NF-κB/AMPK Signal Pathway. Nutrients. 2022; 14(19):4194. https://doi.org/10.3390/nu14194194
Chicago/Turabian StyleWei, Ying, Mingliang Li, Zhiyuan Feng, Di Zhang, Meiling Sun, Yong Wang, and Xiangning Chen. 2022. "The Protective Effects of Corn Oligopeptides on Acute Alcoholic Liver Disease by Inhibiting the Activation of Kupffer Cells NF-κB/AMPK Signal Pathway" Nutrients 14, no. 19: 4194. https://doi.org/10.3390/nu14194194