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Article

Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle

1
Laboratory of Clinical Examination and Sports Medicine, Department of Clinical Medicine, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
2
Department of Sports Medicine Analysis, Open Facility Network Office, Organization for Open Facility Initiatives, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
3
Doctoral Program in Sports Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
4
Experimental Pathology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
5
Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
6
Department of Sport Science and Research, Japan Institute of Sports Sciences, 3-15-1 Nishigaoka, Kita-ku, Tokyo 115-0056, Japan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Juan Del Coso
Nutrients 2022, 14(11), 2239; https://doi.org/10.3390/nu14112239
Received: 2 May 2022 / Revised: 24 May 2022 / Accepted: 25 May 2022 / Published: 27 May 2022
(This article belongs to the Section Nutrition and Metabolism)
Recently, fasting has been spotlighted from a healthcare perspective. However, the de-tailed biological mechanisms and significance by which the effects of fasting confer health benefits are not yet clear. Due to certain advantages of the zebrafish as a vertebrate model, it is widely utilized in biological studies. However, the biological responses to nutrient metabolism within zebrafish skeletal muscles have not yet been amply reported. Therefore, we aimed to reveal a gene expression profile in zebrafish skeletal muscles in response to fasting-refeeding. Accordingly, mRNA-sequencing and bioinformatics analysis were performed to examine comprehensive gene expression changes in skeletal muscle tissues during fasting-refeeding. Our results produced a novel set of nutrition-related genes under a fasting-refeeding protocol. Moreover, we found that five genes were dramatically upregulated in each fasting (for 24 h) and refeeding (after 3 h), exhibiting a rapid response to the provided conditional changes. The assessment of the gene length revealed that the gene set whose expression was elevated only after 3 h of refeeding had a shorter length, suggesting that nutrition-related gene function is associated with gene length. Taken together, our results from the bioinformatics analyses provide new insights into biological mechanisms induced by fasting-refeeding conditions within zebrafish skeletal muscle. View Full-Text
Keywords: fasting; refeeding; skeletal muscle; zebrafish; mRNA-sequencing; gene length fasting; refeeding; skeletal muscle; zebrafish; mRNA-sequencing; gene length
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MDPI and ACS Style

Sugasawa, T.; Komine, R.; Manevich, L.; Tamai, S.; Takekoshi, K.; Kanki, Y. Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle. Nutrients 2022, 14, 2239. https://doi.org/10.3390/nu14112239

AMA Style

Sugasawa T, Komine R, Manevich L, Tamai S, Takekoshi K, Kanki Y. Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle. Nutrients. 2022; 14(11):2239. https://doi.org/10.3390/nu14112239

Chicago/Turabian Style

Sugasawa, Takehito, Ritsuko Komine, Lev Manevich, Shinsuke Tamai, Kazuhiro Takekoshi, and Yasuharu Kanki. 2022. "Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle" Nutrients 14, no. 11: 2239. https://doi.org/10.3390/nu14112239

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