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Article

Rosa canina L. Can Restore Endoplasmic Reticulum Alterations, Protein Trafficking and Membrane Integrity in a Dextran Sulfate Sodium-Induced Inflammatory Bowel Disease Phenotype

1
Department of Biochemistry, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany
2
Laboratory of Functional Physiology and Valorization of Bioresources, Higher Institute of Biotechnology of Beja, University of Jendouba, Beja 7800, Tunisia
3
Faculty of Sciences of Bizerte, University of Carthage, Zarzouna 7021, Tunisia
4
Department of Natural Sciences, Lebanese American University, Beirut 1102-2801, Lebanon
*
Author to whom correspondence should be addressed.
Sharing senior authorship.
Academic Editor: Elad Tako
Nutrients 2021, 13(2), 441; https://doi.org/10.3390/nu13020441
Received: 27 November 2020 / Revised: 21 January 2021 / Accepted: 26 January 2021 / Published: 29 January 2021
Rosa canina L. is a natural polyphenol-rich medicinal plant that exhibits antioxidant and anti-inflammatory activities. Recent in vivo studies have demonstrated that a methanol extract of Rosa canina L. (RCME) has reversed an inflammatory bowel disease (IBD)-like phenotype that has been triggered by dextran sulfate sodium (DSS) in mice. In the current study, we investigated the effects of RCME on perturbations of cellular mechanisms induced by DSS-treatment of intestinal Caco-2 cells, including stress response in the endoplasmic reticulum (ER), protein trafficking and sorting as well as lipid rafts integrity and functional capacities of an intestinal enzyme. 6 days post-confluent cells were treated for 24 h with DSS (3%) or simultaneously with DSS (3%) and RCME (100 µg/mL) or exclusively with RCME (100 µg/mL) or not treated. The results obtained demonstrate the ability of RCME to counteract the substantial increase in the expression levels of several ER stress markers in DSS-treated cells. Concomitantly, the delayed trafficking of intestinal membrane glycoproteins sucrase-isomaltase (SI) and dipeptidyl peptidase 4 (DPP4) induced by DSS between the ER and the Golgi has been compromised by RCME. Furthermore, RCME restored the partially impaired polarized sorting of SI and DPP4 to the brush border membrane. An efficient sorting mechanism of SI and DPP4 is tightly associated with intact lipid rafts structures in the trans-Golgi network (TGN), which have been distorted by DSS and normalized by RCME. Finally, the enzymatic activities of SI are enhanced in the presence of RCME. Altogether, DSS treatment has triggered ER stress, impaired trafficking and function of membrane glycoproteins and distorted lipid rafts, all of which can be compromised by RCME. These findings indicate that the antioxidants in RCME act at two major sites in Caco-2 cells, the ER and the TGN and are thus capable of maintaining the membrane integrity by correcting the sorting of membrane-associated proteins. View Full-Text
Keywords: Rosa canina methanol extract; polyphenols; inflammatory bowel disease; endoplasmic reticulum stress; protein trafficking; intestinal proteins; sucrase-isomaltase; dipeptidyl peptidase 4; brush border membrane; lipid rafts Rosa canina methanol extract; polyphenols; inflammatory bowel disease; endoplasmic reticulum stress; protein trafficking; intestinal proteins; sucrase-isomaltase; dipeptidyl peptidase 4; brush border membrane; lipid rafts
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MDPI and ACS Style

Wanes, D.; Toutounji, M.; Sebai, H.; Rizk, S.; Naim, H.Y. Rosa canina L. Can Restore Endoplasmic Reticulum Alterations, Protein Trafficking and Membrane Integrity in a Dextran Sulfate Sodium-Induced Inflammatory Bowel Disease Phenotype. Nutrients 2021, 13, 441. https://doi.org/10.3390/nu13020441

AMA Style

Wanes D, Toutounji M, Sebai H, Rizk S, Naim HY. Rosa canina L. Can Restore Endoplasmic Reticulum Alterations, Protein Trafficking and Membrane Integrity in a Dextran Sulfate Sodium-Induced Inflammatory Bowel Disease Phenotype. Nutrients. 2021; 13(2):441. https://doi.org/10.3390/nu13020441

Chicago/Turabian Style

Wanes, Dalanda, Mohamad Toutounji, Hichem Sebai, Sandra Rizk, and Hassan Y. Naim. 2021. "Rosa canina L. Can Restore Endoplasmic Reticulum Alterations, Protein Trafficking and Membrane Integrity in a Dextran Sulfate Sodium-Induced Inflammatory Bowel Disease Phenotype" Nutrients 13, no. 2: 441. https://doi.org/10.3390/nu13020441

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