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Open AccessArticle

A Comprehensive Evaluation of the Impact of Bovine Milk Containing Different Beta-Casein Profiles on Gut Health of Ageing Mice

1
Research Centre for Food and Nutrition, CREA (Council for Agricultural Research and Economics), Via Ardeatina 546, 00178 Rome, Italy
2
Research Centre for Engineering and Agro-Food Processing, CREA (Council for Agricultural Research and Economics), Strada delle Cacce 73, 10135 Turin, Italy
3
Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco (TO), Italy
4
Institute of Sciences of Food Production, CNR, Largo Braccini 2, 10095 Grugliasco (TO), Italy
5
Department of Translational Research on New Technologies in Medicine and Surgery, University of Pisa, Via Savi 10, 56126 Pisa, Italy
*
Author to whom correspondence should be addressed.
The two authors contributed equally for the manuscript.
Nutrients 2020, 12(7), 2147; https://doi.org/10.3390/nu12072147
Received: 17 June 2020 / Revised: 15 July 2020 / Accepted: 17 July 2020 / Published: 19 July 2020
(This article belongs to the Section Nutritional Immunology)
Ageing is often characterised by nutritional deficiencies and functional alterations of the digestive and immune system. The aim of the present study was to analyse the impact of consumption of conventional milk with A1/A2 beta-casein, compared to milk containing only the A2 beta-casein variant, characterised by a protein profile favouring gut health. Twenty-four ageing Balb-c mice (20 months old) were fed for 4 weeks, with either a control diet (CTRL), a diet supplemented with bovine milk containing A1/A2 beta-casein (A1A2) or a diet containing A2/A2 beta-casein (A2A2). Lymphocyte subpopulations, enzymatic activities, cytokine secretion, gut morphology and histopathological alterations were measured in different gut segments, while short-chain fatty acids (SCFAs) content and microbiota composition were evaluated in faecal samples. The A2A2 group showed higher content of faecal SCFAs (in particular, isobutyrate) of intestinal CD4+ and CD19+ lymphocytes in the intraepithelial compartment and improved villi tropism. The A1A2 group showed higher percentages of intestinal TCRγδ+ lymphocytes. Faecal microbiota identified Deferribacteriaceae and Desulfovibrionaceae as the most discriminant families for the A2A2 group, while Ruminococcaceae were associated to the A1A2 group. Taken together, these results suggest a positive role of milk, in particular when containing exclusively A2 beta-casein, on gut immunology and morphology of an ageing mice model. View Full-Text
Keywords: A2 beta-casein; elderly; immunosenescence; gut morphology; gut microbiota; gut inflammation; SCFAs A2 beta-casein; elderly; immunosenescence; gut morphology; gut microbiota; gut inflammation; SCFAs
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Guantario, B.; Giribaldi, M.; Devirgiliis, C.; Finamore, A.; Colombino, E.; Capucchio, M.T.; Evangelista, R.; Motta, V.; Zinno, P.; Cirrincione, S.; Antoniazzi, S.; Cavallarin, L.; Roselli, M. A Comprehensive Evaluation of the Impact of Bovine Milk Containing Different Beta-Casein Profiles on Gut Health of Ageing Mice. Nutrients 2020, 12, 2147.

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