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Open AccessArticle

Coenzyme A and Its Thioester Pools in Obese Zucker and Zucker Diabetic Fatty Rats

1
Department of Food and Life Sciences, Ibaraki University College of Agriculture, 3-21-1 Chuo, Ami, Ibaraki 300-0393, Japan
2
Department of Applied Life Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo 183-8509, Japan
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(2), 417; https://doi.org/10.3390/nu12020417
Received: 13 January 2020 / Revised: 3 February 2020 / Accepted: 3 February 2020 / Published: 6 February 2020
(This article belongs to the Section Nutrition and Metabolism)
Feeding behavior is closely related to hypothalamic malonyl-CoA level in the brain and diet-induced obesity affects total CoA pools in liver. Herein, we performed a comprehensive analysis of the CoA pools formed in thirteen tissues of Zucker and Zucker diabetic fatty (ZDF) rats. Hypothalamic malonyl-CoA levels in obese rats remained low and were almost the same as those of lean rats, despite obese rats having much higher content of leptin, insulin, and glucose in their sera. Regardless of the fa-genotypes, larger total CoA pools were formed in the livers of ZDF rats and the size of hepatic total CoA pools in Zucker rats showed almost one tenth of the size of ZDF rats. The decreased total CoA pool sizes in Zucker rats was observed in the brown adipose tissues, while ZDF-fatty rats possessed 6% of total CoA pool in the lean rats in response to fa deficiency. This substantially lower CoA content in the obese rats would be disadvantageous to non-shivering thermogenesis. Thus, comparing the intracellular CoA behaviors between Zucker and ZDF rats, as well as the lean and fatty rats of each strain would help to elucidate features of obesity and type 2 diabetes in combination with result (s) of differential gene expression analysis and/or comparative genomics. View Full-Text
Keywords: coenzyme A; acetyl-CoA; malonyl-CoA; Zucker rat; ZDF rat coenzyme A; acetyl-CoA; malonyl-CoA; Zucker rat; ZDF rat
MDPI and ACS Style

Chohnan, S.; Matsuno, S.; Shimizu, K.; Tokutake, Y.; Kohari, D.; Toyoda, A. Coenzyme A and Its Thioester Pools in Obese Zucker and Zucker Diabetic Fatty Rats. Nutrients 2020, 12, 417.

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