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Open AccessArticle

Inhibitory Effect of Centella asiatica Extract on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice

1
Department of Food and Nutrition, KC University, Seoul 07661, Korea
2
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul 01811, Korea
3
Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Seoul 27478, Korea
*
Author to whom correspondence should be addressed.
These authors are co-first author.
Nutrients 2020, 12(2), 411; https://doi.org/10.3390/nu12020411
Received: 27 December 2019 / Revised: 28 January 2020 / Accepted: 31 January 2020 / Published: 5 February 2020
Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the Centella asiatica ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm2, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.
Keywords: atopic dermatitis; Centella asiatica ethanol extract; madecassoside; asiaticoside; anti-inflammation atopic dermatitis; Centella asiatica ethanol extract; madecassoside; asiaticoside; anti-inflammation
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Lee, Y.; Choi, H.K.; N’deh, K.P.U.; Choi, Y.-J.; Fan, M.; Kim, E.-K.; Chung, K.-H.; An, J.H. Inhibitory Effect of Centella asiatica Extract on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice. Nutrients 2020, 12, 411.

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