A Comparison of Gene Expression Changes in the Blood of Individuals Consuming Diets Supplemented with Olives, Nuts or Long-Chain Omega-3 Fatty Acids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Analysis of PBMC Transcriptomic Studies
2.2. Pathway Enrichment Analysis
2.3. Gene-Transcription Factors Interaction Analysis
2.4. Gene-miRNA Interaction Analysis
2.5. Gene-Disease Association Analysis
2.6. Diet and Dementia Analysis
3. Results
3.1. Gene Expression Comparison of the Diet Supplements
3.2. Pathway Enrichment Analysis
3.3. Gene-Transcription Factors Interaction Analysis
3.4. Gene-miRNA Interaction Analysis
3.5. Gene-Disease Association Analysis
3.6. Diets and Dementia Analysis
4. Discussion
4.1. Regulation of Gene Expression by Components of a Mediterranean Diet
4.2. Gene Expression Regulation by Transcription Factors and miRNA in Mediterranean Diets
4.3. Mediterranean Diets and Disease Association
4.4. Limitations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Diets | Datasets | Platform | Sample # | Age | Sex (%F) | Health | Reference |
---|---|---|---|---|---|---|---|
Mediterranean diet + Olive oil | GSE28358 | GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array | 12 | 62 ± 8 | 45 | High risk of coronary artery disease | [36] |
Olive oil | GSE75025 | GPL10558 Illumina HumanHT-12 V4.0 expression beadchip | 12 | 29 ± 2 | 50 | Healthy | [37] |
Olive leaf extract | GSE87300 | GPL13667 [HG-U219] Affymetrix Human Genome U219 Array | 15 | 32 | 0 | Healthy | [38] |
Mediterranean diet + Nuts | GSE28358 | GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array | 10 | 63 ± 6 | 45 | High risk of coronary artery disease | [36] |
Fish oil 3 weeks | GSE48368 | GPL10558 Illumina HumanHT-12 V4.0 expression beadchip | 17 | 27.2 ± 6.9 | 71 | Healthy | [39] |
Fish oil 7 weeks | GSE48368 | GPL10558 Illumina HumanHT-12 V4.0 expression beadchip | 17 | 27.2 ± 6.9 | 71 | Healthy | [39] |
EPA + DHA | GSE12375 | GPL7144 NuGO array (human) NuGO_Hs1a520180 | 23 | 69.9 (67-76) | 35 | Healthy | [40] |
EPA + DHA | E-MTAB-48 | A-AFFY-111—Affymetrix Custom Array | 23 | 69.9 (67-76) | 35 | Healthy | [40] |
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Bottero, V.; Potashkin, J.A. A Comparison of Gene Expression Changes in the Blood of Individuals Consuming Diets Supplemented with Olives, Nuts or Long-Chain Omega-3 Fatty Acids. Nutrients 2020, 12, 3765. https://doi.org/10.3390/nu12123765
Bottero V, Potashkin JA. A Comparison of Gene Expression Changes in the Blood of Individuals Consuming Diets Supplemented with Olives, Nuts or Long-Chain Omega-3 Fatty Acids. Nutrients. 2020; 12(12):3765. https://doi.org/10.3390/nu12123765
Chicago/Turabian StyleBottero, Virginie, and Judith A. Potashkin. 2020. "A Comparison of Gene Expression Changes in the Blood of Individuals Consuming Diets Supplemented with Olives, Nuts or Long-Chain Omega-3 Fatty Acids" Nutrients 12, no. 12: 3765. https://doi.org/10.3390/nu12123765