Antibiotic Treatment Does Not Ameliorate the Metabolic Changes in Rats Presenting Dysbiosis After Consuming a High Fructose Diet
by
Ariel Bier 1,2, Rawan Khasbab 1,2, Yael Haberman 2,3,4, Tzipi Braun 3, Rotem Hadar 3, Katya Sosnovski 3, Amnon Amir 3, Avshalom Leibowitz 1,2,*,† and Ehud Grossman 1,2,†
Ariel Bier 1,2, Rawan Khasbab 1,2, Yael Haberman 2,3,4, Tzipi Braun 3, Rotem Hadar 3, Katya Sosnovski 3, Amnon Amir 3, Avshalom Leibowitz 1,2,*,† and Ehud Grossman 1,2,†
1
Internal Medicine D and Hypertension Unit, The Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan 5265601, Israel
2
Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv 69978, Israel
3
The Chaim Sheba Medical Center, Tel-Hashomer, Ramat-Gan 5265601, Israel
4
Cincinnati Children’s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
*
Author to whom correspondence should be addressed.
†
Both authors contributed equally to the manuscript.
Nutrients 2020, 12(1), 203; https://doi.org/10.3390/nu12010203
Received: 26 December 2019 / Revised: 6 January 2020 / Accepted: 9 January 2020 / Published: 13 January 2020
(This article belongs to the Special Issue Diet, Gut Microbiota and Metabolic Disorders)
High fructose consumption is one of the hallmarks of Western diets and has been found to induce MeS symptoms in parallel to gut microbial dysbiosis. However, the causality between those two is still elusive. Here, we studied whether a significant modification of gut microbial composition by antibiotics can influence the fructose-induced metabolic changes. Male Sprague-Dawley (SD) rats were divided into four groups including controls, controls + antibiotics, high fructose diet (HFrD, 60% fructose), HFrD + antibiotics (n = 7–8 in each group) for a period of 8-weeks. The high fructose diet increased blood pressure (BP), triglyceride (TG), fatty liver and the expression of hepatic genes related to lipogenesis, and fructose transport and metabolism. In addition, fructose changed the microbial composition and increased acetic and butyric acids in fecal samples but not in the blood. Antibiotic treatment significantly reduced microbial diversity and modified the microbial composition in the samples. However, minimal or no effect was seen in the metabolic phenotypes. In conclusion, high fructose consumption (60%) induced metabolic changes and dysbiosis in rats. However, antibiotic treatment did not reverse the metabolic phenotype. Therefore, the metabolic changes are probably independent of a specific microbiome profile.
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Keywords:
fructose; metabolic syndrome; microbiome; dysbiosis
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MDPI and ACS Style
Bier, A.; Khasbab, R.; Haberman, Y.; Braun, T.; Hadar, R.; Sosnovski, K.; Amir, A.; Leibowitz, A.; Grossman, E. Antibiotic Treatment Does Not Ameliorate the Metabolic Changes in Rats Presenting Dysbiosis After Consuming a High Fructose Diet. Nutrients 2020, 12, 203.
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