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Docosahexaenoic and Eicosapentaenoic Acids Prevent Altered-Muc2 Secretion Induced by Palmitic Acid by Alleviating Endoplasmic Reticulum Stress in LS174T Goblet Cells

by 1,2,3,4, 1,2,3, 1,2,3,* and 1,2,3,*
1
University of Bourgogne Franche-Comté, UFR Sciences de la Vie, de la Terre et de l’Environnement, Lipides Nutrition Cancer UMR U1231, 6 Boulevard Gabriel, F-21000 Dijon, France
2
INSERM, Lipides Nutrition Cancer UMR1231, F-21000 Dijon, France
3
LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, F-21000 Dijon, France
4
Valorex, La Messayais, 35210 Combourtillé, France
*
Authors to whom correspondence should be addressed.
Nutrients 2019, 11(9), 2179; https://doi.org/10.3390/nu11092179
Received: 26 July 2019 / Revised: 6 September 2019 / Accepted: 8 September 2019 / Published: 11 September 2019
Diets high in saturated fatty acids (FA) represent a risk factor for the development of obesity and associated metabolic disorders, partly through their impact on the epithelial cell barrier integrity. We hypothesized that unsaturated FA could alleviate saturated FA-induced endoplasmic reticulum (ER) stress occurring in intestinal secretory goblet cells, and consequently the reduced synthesis and secretion of mucins that form the protective mucus barrier. To investigate this hypothesis, we treated well-differentiated human colonic LS174T goblet cells with palmitic acid (PAL)—the most commonly used inducer of lipotoxicity in in vitro systems—or n-9, n-6, or n-3 unsaturated fatty acids alone or in co-treatment with PAL, and measured the impact of such treatments on ER stress and Muc2 production. Our results showed that only eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids protect goblet cells against ER stress-mediated altered Muc2 secretion induced by PAL, whereas neither linolenic acid nor n-9 and n-6 FA are able to provide such protection. We conclude that EPA and DHA could represent potential therapeutic nutrients against the detrimental lipotoxicity of saturated fatty acids, associated with type 2 diabetes and obesity or inflammatory bowel disease. These in vitro data remain to be explored in vivo in a context of dietary obesity. View Full-Text
Keywords: palmitic acid; n-3 fatty acids; gastrointestinal barrier; intestinal goblet cells; endoplasmic reticulum stress; Muc2 secretion palmitic acid; n-3 fatty acids; gastrointestinal barrier; intestinal goblet cells; endoplasmic reticulum stress; Muc2 secretion
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Escoula, Q.; Bellenger, S.; Narce, M.; Bellenger, J. Docosahexaenoic and Eicosapentaenoic Acids Prevent Altered-Muc2 Secretion Induced by Palmitic Acid by Alleviating Endoplasmic Reticulum Stress in LS174T Goblet Cells. Nutrients 2019, 11, 2179.

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