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Hyperglycemia Changes Expression of Key Adipogenesis Markers (C/EBPα and PPARᵞ)and Morphology of Differentiating Human Visceral Adipocytes

1
Department of Medical Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland
2
Department of Internal Medicine, Diabetology and Clinical Pharmacology, Medical University of Lodz, 92-213 Lodz, Poland
3
Department of Thermobiology, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland
4
Laboratory of Laser Molecular Spectroscopy, Lodz University of Technology, 93-590 Lodz, Poland
5
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-647 Lodz, Poland
6
Central Teaching Hospital of the Medical University of Lodz, 92-213 Lodz, Poland
7
Department of Nucleic Acids Biochemistry, Medical University of Lodz, 92-213 Lodz, Poland
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2019, 11(8), 1835; https://doi.org/10.3390/nu11081835
Received: 6 July 2019 / Revised: 31 July 2019 / Accepted: 6 August 2019 / Published: 8 August 2019
(This article belongs to the Special Issue Nutrition, Genes and Signaling Pathways)
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Abstract

Disturbances in adipose tissue significantly contribute to the development of metabolic disorders, which are connected with hyperglycemia (HG) and underlain by epigenetics-based mechanisms. Therefore, we aimed to evaluate the effect of hyperglycemia on proliferating, differentiating and maturating human visceral pre/adipocytes (HPA-v). Three stages of cell culture were conducted under constant or variable glycemic conditions. Adipogenesis progress was assessed using BODIPY 505/515 staining. Lipid content typical for normal and hyperglycemic conditions of adipocytes was analyzed using Raman spectroscopy and imaging. Expression of adipogenic markers, PPARγ and C/EBPα, was determined at the mRNA and protein levels. We also examined expression of miRNAs proven to target PPARγ (miR-34a-5p) and C/EBPα (miR-137-3p), employing TaqMan Low-Density Arrays (TLDA) cards. Hyperglycemia altered morphology of differentiating HPA-v in relation to normoglycemia by accelerating the formation of lipid droplets and making their numbers and volume increase. Raman results confirmed that the qualitative and quantitative lipid composition under normal and hyperglycemic conditions were different, and that the number of lipid droplets increased in (HG)-treated cells. Expression profiles of both examined genes markedly changed either during adipogenesis under physiological and hyperglycemic conditions, orat particular stages of adipogenesis upon chronic and/or variable glycemia. Expression levels of PPARγ seemed to correspond to some expression changes of miR-34a-5p. miR-137-3p, whose expression was rather stable throughout the culture, did not seem to affect C/EBPα. Our observations revealed that chronic and intermittent hyperglycemia change the morphology of visceral pre/adipocytes during adipogenesis. Moreover, hyperglycemia may utilize miR-34a-5p to induce some expression changes in PPARγ. View Full-Text
Keywords: visceral preadipocytes; adipocytes; adipogenesis; PPARγ; C/EBPα; miR-34a-5p; miR-137-3p; hyperglycemia; diabetes visceral preadipocytes; adipocytes; adipogenesis; PPARγ; C/EBPα; miR-34a-5p; miR-137-3p; hyperglycemia; diabetes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Świderska, E.; Podolska, M.; Strycharz, J.; Szwed, M.; Abramczyk, H.; Brożek-Płuska, B.; Wróblewski, A.; Szemraj, J.; Majsterek, I.; Drzewoski, J.; Śliwińska, A. Hyperglycemia Changes Expression of Key Adipogenesis Markers (C/EBPα and PPARᵞ)and Morphology of Differentiating Human Visceral Adipocytes. Nutrients 2019, 11, 1835.

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