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Intra-Abdominal Fat Adipocyte Hypertrophy through a Progressive Alteration of Lipolysis and Lipogenesis in Metabolic Syndrome Rats

1
Department of Pathology, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano 1, Sección XVI, Tlalpan, México City 14080, Mexico
2
Department of Physiology, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano 1, Sección XVI, Tlalpan, México City 14080, Mexico
3
Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, Vasco de Quiroga 15, Sección XVI, Tlalpan, México City 14000, Mexico
4
Facultad de Medicina y Veterinaria y Zootecnia, Department of Physiology and Pharmacology UNAM, Av. Universidad 3000, Coyoacán, México City 04510, Mexico
*
Author to whom correspondence should be addressed.
Pérez-Torres I and Gutiérrez-Alvarez Y, share the first authorship of this paper.
Nutrients 2019, 11(7), 1529; https://doi.org/10.3390/nu11071529
Received: 29 May 2019 / Revised: 27 June 2019 / Accepted: 3 July 2019 / Published: 5 July 2019
(This article belongs to the Special Issue Diet and Metabolic Syndromes)
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Abstract

This study evaluates the progressive participation of enzymes involved in lipolysis and lipogenesis, leading to adipocyte hypertrophy in a metabolic syndrome (MS) rat model caused by chronic consumption of 30% sucrose in drinking water. A total of 70 male Wistar rats were divided into two groups: C and MS. Each of these groups were then subdivided into five groups which were sacrificed as paired groups every month from the beginning of the treatment until 5 months. The intra-abdominal fat was dissected, and the adipocytes were extracted. Lipoprotein lipase (LPL), hormone-sensitive lipase (HSL), protein kinases A (PKA), and perilipin A expressions were determined. The LPL and HSL activities were evaluated by spectrophotometry. Histological staining was performed in adipose tissue. Significant increases were observed in blood pressure, HOMA-IR, leptin, triglycerides, insulin, intra-abdominal fat, and number of fat cells per field (p = 0.001) and in advanced glycosylation products, adipocyte area, LPL, HSL activities and/or expression (p ≤ 0.01) in the MS groups progressively from the third month onward. Lipogenesis and lipolysis were increased by LPL activity and HSL activity and/or expression. This was associated with hyperinsulinemia and release of non-esterified fatty acids causing a positive feedback loop that contributes to the development of adipocyte hypertrophy. View Full-Text
Keywords: metabolic syndrome; adipocyte; hypertrophy; lipoprotein lipase; hormone-sensitive lipase; perilipin A metabolic syndrome; adipocyte; hypertrophy; lipoprotein lipase; hormone-sensitive lipase; perilipin A
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Pérez-Torres, I.; Gutiérrez-Alvarez, Y.; Guarner-Lans, V.; Díaz-Díaz, E.; Manzano Pech, L.; Caballero-Chacón, S.C. Intra-Abdominal Fat Adipocyte Hypertrophy through a Progressive Alteration of Lipolysis and Lipogenesis in Metabolic Syndrome Rats. Nutrients 2019, 11, 1529.

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