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Open AccessArticle

Activity-Guided Fractionation of Red Fruit Extracts for the Identification of Compounds Influencing Glucose Metabolism

1
Institut für Lebensmittelchemie, Technische Universität Braunschweig, Schleinitzstraße 20, D-38106 Braunschweig, Germany
2
Department of Chemistry, Division of Food Chemistry and Toxicology, Technische Universität Kaiserslautern, Erwin-Schrödinger-Straße 52, D-67663 Kaiserslautern, Germany
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(5), 1166; https://doi.org/10.3390/nu11051166
Received: 30 April 2019 / Revised: 20 May 2019 / Accepted: 22 May 2019 / Published: 24 May 2019
(This article belongs to the Special Issue Dietary Bioactives and Human Health)
An activity-guided search for compounds influencing glucose metabolism in extracts from aronia (Aronia melanocarpa, A.), pomegranate (Punica granatum L., P.), and red grape (Vitis vinifera, RG) was carried out. The three extracts were fractionated by means of membrane chromatography to separate the anthocyanins from other noncolored phenolic compounds (copigments). In addition, precipitation with hexane was performed to isolate the polymers (PF). The anthocyanin and copigment fractions (AF, CF) of aronia, pomegranate, and red grape were furthermore fractionated with high-performance countercurrent chromatography (HPCCC) and the subfractions were characterized by HPLC-PDA-MS/MS analyses. Each of the (sub-)fractions was examined by in vitro-tests, i.e., the inhibition of the activity of α-amylase and α-glucosidase. On the basis of this screening, several potent inhibitors of the two enzymes could be identified, which included flavonols (e.g., quercetin), ellagitannins (e.g., pedunculagin), and anthocyanins (e.g., delphinidin-3-glucoside and petunidin-3-glucoside). In the α-glucosidase assay all of the examined fractions and subfractions of the fruit extracts were more active than the positive control acarbose. View Full-Text
Keywords: aronia; pomegranate; red grape; polyphenols; HPLC-PDA-MS/MS; α-amylase; α-glucosidase; high-performance countercurrent chromatography (HPCCC) aronia; pomegranate; red grape; polyphenols; HPLC-PDA-MS/MS; α-amylase; α-glucosidase; high-performance countercurrent chromatography (HPCCC)
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MDPI and ACS Style

Ostberg-Potthoff, J.J.; Berger, K.; Richling, E.; Winterhalter, P. Activity-Guided Fractionation of Red Fruit Extracts for the Identification of Compounds Influencing Glucose Metabolism. Nutrients 2019, 11, 1166. https://doi.org/10.3390/nu11051166

AMA Style

Ostberg-Potthoff JJ, Berger K, Richling E, Winterhalter P. Activity-Guided Fractionation of Red Fruit Extracts for the Identification of Compounds Influencing Glucose Metabolism. Nutrients. 2019; 11(5):1166. https://doi.org/10.3390/nu11051166

Chicago/Turabian Style

Ostberg-Potthoff, Johanna J.; Berger, Kirsten; Richling, Elke; Winterhalter, Peter. 2019. "Activity-Guided Fractionation of Red Fruit Extracts for the Identification of Compounds Influencing Glucose Metabolism" Nutrients 11, no. 5: 1166. https://doi.org/10.3390/nu11051166

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