Ganoderma lucidum Extract Reduces the Motility of Breast Cancer Cells Mediated by the RAC–Lamellipodin Axis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Whole Mushroom Ganoderma Lucidum Extract (GLE)
2.2. Cell Culture
2.3. Cell Viability
2.4. Wash Out Assays
2.5. Wound Healing Assay
2.6. Invasion Assay
2.7. Rac Activity Assay
2.8. Western Blot Analysis
2.9. Immunofluorescence
2.10. Statistical Analysis
3. Results
3.1. GLE Selectively Inhibits Cancer Cell Viability
3.2. GLE inhibits Cancer Cell Migration and Invasion
3.3. GLE Affects Rac Activity
3.4. GLE Decreases Expression of Proteins Involved in Cell Migration
3.5. GLE Affects Lamellipodia Formation in MDA-MB-231 Cells
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Acevedo-Díaz, A.; Ortiz-Soto, G.; Suárez-Arroyo, I.J.; Zayas-Santiago, A.; Martínez Montemayor, M.M. Ganoderma lucidum Extract Reduces the Motility of Breast Cancer Cells Mediated by the RAC–Lamellipodin Axis. Nutrients 2019, 11, 1116. https://doi.org/10.3390/nu11051116
Acevedo-Díaz A, Ortiz-Soto G, Suárez-Arroyo IJ, Zayas-Santiago A, Martínez Montemayor MM. Ganoderma lucidum Extract Reduces the Motility of Breast Cancer Cells Mediated by the RAC–Lamellipodin Axis. Nutrients. 2019; 11(5):1116. https://doi.org/10.3390/nu11051116
Chicago/Turabian StyleAcevedo-Díaz, Ariana, Gabriela Ortiz-Soto, Ivette J. Suárez-Arroyo, Astrid Zayas-Santiago, and Michelle M. Martínez Montemayor. 2019. "Ganoderma lucidum Extract Reduces the Motility of Breast Cancer Cells Mediated by the RAC–Lamellipodin Axis" Nutrients 11, no. 5: 1116. https://doi.org/10.3390/nu11051116
APA StyleAcevedo-Díaz, A., Ortiz-Soto, G., Suárez-Arroyo, I. J., Zayas-Santiago, A., & Martínez Montemayor, M. M. (2019). Ganoderma lucidum Extract Reduces the Motility of Breast Cancer Cells Mediated by the RAC–Lamellipodin Axis. Nutrients, 11(5), 1116. https://doi.org/10.3390/nu11051116