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Article

The Type 2 Diabetes Susceptibility PROX1 Gene Variants Are Associated with Postprandial Plasma Metabolites Profile in Non-Diabetic Men

1
Clinical Research Centre, Medical University of Bialystok, 15-089 Bialystok, Poland
2
Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-089 Bialystok, Poland
3
Center for Metabolomics and Bioanalysis (CEMBIO), Universidad CEU San Pablo, 28003 Madrid, Spain
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(4), 882; https://doi.org/10.3390/nu11040882
Received: 11 March 2019 / Revised: 4 April 2019 / Accepted: 17 April 2019 / Published: 19 April 2019
(This article belongs to the Special Issue Personalized Nutrition-1)
The prospero homeobox 1 (PROX1) gene may show pleiotropic effects on metabolism. We evaluated postprandial metabolic alterations dependently on the rs340874 genotypes, and 28 non-diabetic men were divided into two groups: high-risk (HR)-genotype (CC-genotype carriers, n = 12, 35.3 ± 9.5 years old) and low-risk (LR)-genotype (allele T carriers, n = 16, 36.3 ± 7.0 years old). Subjects participated in two meal-challenge-tests with high-carbohydrate (HC, carbohydrates 89%) and normo-carbohydrate (NC, carbohydrates 45%) meal intake. Fasting and 30, 60, 120, and 180 min after meal intake plasma samples were fingerprinted by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). In HR-genotype men, the area under the curve (AUC) of acetylcarnitine levels was higher after the HC-meal [+92%, variable importance in the projection (VIP) = 2.88] and the NC-meal (+55%, VIP = 2.00) intake. After the NC-meal, the HR-risk genotype carriers presented lower AUCs of oxidized fatty acids (−81–66%, VIP = 1.43–3.16) and higher linoleic acid (+80%, VIP = 2.29), while after the HC-meal, they presented lower AUCs of ornithine (−45%, VIP = 1.83), sphingosine (−48%, VIP = 2.78), linoleamide (−45%, VIP = 1.51), and several lysophospholipids (−40–56%, VIP = 1.72–2.16). Moreover, lower AUC (−59%, VIP = 2.43) of taurocholate after the HC-meal and higher (+70%, VIP = 1.42) glycodeoxycholate levels after the NC-meal were observed. Our results revealed differences in postprandial metabolites from inflammatory and oxidative stress pathways, bile acids signaling, and lipid metabolism in PROX1 HR-genotype men. Further investigations of diet–genes interactions by which PROX1 may promote T2DM development are needed. View Full-Text
Keywords: nutrigenetics; nutrimetabolomics; high-carbohydrate meal; normo-carbohydrate meal; postprandial metabolic fingerprinting; ultra-high performance liquid chromatography; PROX1 gene; type 2 diabetes mellitus risk nutrigenetics; nutrimetabolomics; high-carbohydrate meal; normo-carbohydrate meal; postprandial metabolic fingerprinting; ultra-high performance liquid chromatography; PROX1 gene; type 2 diabetes mellitus risk
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MDPI and ACS Style

Adamska-Patruno, E.; Godzien, J.; Ciborowski, M.; Samczuk, P.; Bauer, W.; Siewko, K.; Gorska, M.; Barbas, C.; Kretowski, A. The Type 2 Diabetes Susceptibility PROX1 Gene Variants Are Associated with Postprandial Plasma Metabolites Profile in Non-Diabetic Men. Nutrients 2019, 11, 882. https://doi.org/10.3390/nu11040882

AMA Style

Adamska-Patruno E, Godzien J, Ciborowski M, Samczuk P, Bauer W, Siewko K, Gorska M, Barbas C, Kretowski A. The Type 2 Diabetes Susceptibility PROX1 Gene Variants Are Associated with Postprandial Plasma Metabolites Profile in Non-Diabetic Men. Nutrients. 2019; 11(4):882. https://doi.org/10.3390/nu11040882

Chicago/Turabian Style

Adamska-Patruno, Edyta, Joanna Godzien, Michal Ciborowski, Paulina Samczuk, Witold Bauer, Katarzyna Siewko, Maria Gorska, Coral Barbas, and Adam Kretowski. 2019. "The Type 2 Diabetes Susceptibility PROX1 Gene Variants Are Associated with Postprandial Plasma Metabolites Profile in Non-Diabetic Men" Nutrients 11, no. 4: 882. https://doi.org/10.3390/nu11040882

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