Protective Effects of CISD2 and Influence of Curcumin on CISD2 Expression in Aged Animals and Inflammatory Cell Model
Abstract
1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Animals
2.3. Curcumin Treatment of Mice
2.4. Cells
2.5. Curcumin Treatment of Lipopolysaccharide (LPS)-Challenged Neural Cells
2.6. Reverse-Transcription Polymerase Chain Reaction and Real-Time Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR)
2.7. RNA Interference and Transfection
2.8. Immunoblotting of CISD2
2.9. Chemicals and Fluorescent Dyes for the Study of Mitochondrial Function
2.10. Cell Viability
2.11. Statistical Analysis
3. Results
3.1. Aging-Driven Decline in CISD2 Expression in Naturally Aged Mice and Curcumin Protected against Aging-Driven Decline in CISD2 Expression as Well as Aging-Augmented Inflammatory Responses in Long-Term Cultures of Neural Cells
3.2. Knockdown of CISD2 Expression in Neural Cells Led to High No Production with a Strong Inflammatory Reaction, Enhanced Apoptosis, and Attenuated Cell Survival
3.3. Curcumin Enhanced CISD2 mRNA and Protein Expression In Vivo and In Vitro via JAK/STAT Signaling Pathways
3.4. Curcumin Enhanced an LPS-Triggered Reduction in Mitochondrial Membrane Potential In Vitro
3.5. Curcumin Protected against LPS-Augmented Reactive Oxygen Species (ROS) Formation In Vitro
3.6. Curcumin Inhibited LPS-Induced Apoptosis In Vitro
3.7. Curcumin Enhanced Cell Survival in Non-Stressed and Injury-Challenged Neural Cells
3.8. Attenuated Survival Enhancing Effect of Curcumin in Non-Stressed as Well as LPS-Challenged Neural Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Orientation | Sequence |
---|---|---|
CISD2 | forward | 5′-AAAATCCCAAGGTGGTGAATGA-3′ |
reverse | 5′-GGACCGCCAGCACCTACA-3′ | |
Cyclophilin | forward | 5′-ATGGTCAACCCCACCGTGT-3′ |
reverse | 5′-CGTGTGAAGTCACCACCCT-3′ | |
BCL2 | forward | 5′-TGGGATGCCTTTGTGGAACT-3′ |
reverse | 5′-CAGCCAGGAGAAATCAAACAGA-3′ | |
iNOS | forward | 5′-CCTCAGTTCTGCGCCTTTG-3′ |
reverse | 5′-GTTCGTCCCCTTCTCCTGTTG-3′ | |
RANTES | forward | 5′-TGCCCACGTCAAGGAGTATTT-3′ |
reverse | 5′-GGCGGTTCCTTCGAGTGA-3′ |
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Lin, C.-C.; Chiang, T.-H.; Sun, Y.-Y.; Lin, M.-S. Protective Effects of CISD2 and Influence of Curcumin on CISD2 Expression in Aged Animals and Inflammatory Cell Model. Nutrients 2019, 11, 700. https://doi.org/10.3390/nu11030700
Lin C-C, Chiang T-H, Sun Y-Y, Lin M-S. Protective Effects of CISD2 and Influence of Curcumin on CISD2 Expression in Aged Animals and Inflammatory Cell Model. Nutrients. 2019; 11(3):700. https://doi.org/10.3390/nu11030700
Chicago/Turabian StyleLin, Chai-Ching, Tien-Huang Chiang, Yu-Yo Sun, and Muh-Shi Lin. 2019. "Protective Effects of CISD2 and Influence of Curcumin on CISD2 Expression in Aged Animals and Inflammatory Cell Model" Nutrients 11, no. 3: 700. https://doi.org/10.3390/nu11030700
APA StyleLin, C.-C., Chiang, T.-H., Sun, Y.-Y., & Lin, M.-S. (2019). Protective Effects of CISD2 and Influence of Curcumin on CISD2 Expression in Aged Animals and Inflammatory Cell Model. Nutrients, 11(3), 700. https://doi.org/10.3390/nu11030700