Next Article in Journal
Adherence to the Mediterranean Diet and Its Association with Body Composition and Physical Fitness in Spanish University Students
Next Article in Special Issue
Assessment of Vitamin C and Antioxidant Profiles in Saliva and Serum in Patients with Periodontitis and Ischemic Heart Disease
Previous Article in Journal
Dietary Patterns and Risk of Obesity and Early Childhood Caries in Australian Toddlers: Findings from an Australian Cohort Study
Open AccessArticle

Investigation of Antibacterial and Antiinflammatory Activities of Proanthocyanidins from Pelargonium sidoides DC Root Extract

1
Medical Academy, Lithuanian University of Health Sciences, Sukileliu Ave. 13, LT-50162 Kaunas, Lithuania
2
Latvian Institute of Organic Synthesis, Aizkraukles Str. 21, LV1006 Riga, Latvia
3
Riga Stradins University, Dzirciema Str. 16, LV1007, Latvia
4
Department of Health Sciences, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy
5
Interdisciplinary Research Center of Autoimmune Diseases, Center for Translational Research on Autoimmune and Allergic Diseases–CAAD, C.so Trieste 15A, 28100 Novara, Italy
6
Clinic of dental and oral pathology, LSMU Hospital, Kaunas Clinics, Medical academy, Lithuanian University of Health Sciences, Eiveniu Str. 2, LT-50161 Kaunas, Lithuania
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(11), 2829; https://doi.org/10.3390/nu11112829
Received: 16 September 2019 / Revised: 30 October 2019 / Accepted: 8 November 2019 / Published: 19 November 2019
The study explores antibacterial, antiinflammatory and cytoprotective capacity of Pelargonium sidoides DC root extract (PSRE) and proanthocyanidin fraction from PSRE (PACN) under conditions characteristic for periodontal disease. Following previous finding that PACN exerts stronger suppression of Porphyromonas gingivalis compared to the effect on commensal Streptococcus salivarius, the current work continues antibacterial investigation on Staphylococcus aureus, Staphylococcus epidermidis, Aggregatibacter actinomycetemcomitans and Escherichia coli. PSRE and PACN are also studied for their ability to prevent gingival fibroblast cell death in the presence of bacteria or bacterial lipopolysaccharide (LPS), to block LPS- or LPS + IFNγ-induced release of inflammatory mediators, gene expression and surface antigen presentation. Both PSRE and PACN were more efficient in suppressing Staphylococcus and Aggregatibacter compared to Escherichia, prevented A. actinomycetemcomitans- and LPS-induced death of fibroblasts, decreased LPS-induced release of interleukin-8 and prostaglandin E2 from fibroblasts and IL-6 from leukocytes, blocked expression of IL-1β, iNOS, and surface presentation of CD80 and CD86 in LPS + IFNγ-treated macrophages, and IL-1β and COX-2 expression in LPS-treated leukocytes. None of the investigated substances affected either the level of secretion or expression of TNFα. In conclusion, PSRE, and especially PACN, possess strong antibacterial, antiinflammatory and gingival tissue protecting properties under periodontitis-mimicking conditions and are suggestable candidates for treatment of the disease. View Full-Text
Keywords: periodontitis; Pelargonium sidoides DC root extract; proanthocyanidins; bacteriotoxicity; inflammatory cytokines; gene expression; fibroblasts; macrophages; leukocytes periodontitis; Pelargonium sidoides DC root extract; proanthocyanidins; bacteriotoxicity; inflammatory cytokines; gene expression; fibroblasts; macrophages; leukocytes
Show Figures

Graphical abstract

MDPI and ACS Style

Jekabsone, A.; Sile, I.; Cochis, A.; Makrecka-Kuka, M.; Laucaityte, G.; Makarova, E.; Rimondini, L.; Bernotiene, R.; Raudone, L.; Vedlugaite, E.; Baniene, R.; Smalinskiene, A.; Savickiene, N.; Dambrova, M. Investigation of Antibacterial and Antiinflammatory Activities of Proanthocyanidins from Pelargonium sidoides DC Root Extract. Nutrients 2019, 11, 2829.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop