Whey Protein Concentrate WPC-80 Intensifies Glycoconjugate Catabolism and Induces Oxidative Stress in the Liver of Rats
by
Marta Żebrowska-Gamdzyk 1,2,*,†, Mateusz Maciejczyk 2,3,*,†
, Anna Zalewska 4, Katarzyna Guzińska-Ustymowicz 5, Anna Tokajuk 2 and Halina Car 2
Marta Żebrowska-Gamdzyk 1,2,*,†, Mateusz Maciejczyk 2,3,*,†, Anna Zalewska 4, Katarzyna Guzińska-Ustymowicz 5, Anna Tokajuk 2 and Halina Car 2
1
Lomza State University of Applied Sciences, 14 Akademicka Street, 18-400 Lomza, Poland
2
Department of Experimental Pharmacology, Medical University of Bialystok, 37 Szpitalna Street, 15-767 Bialystok, Poland
3
Department of Physiology, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, Poland
4
Department of Conservative Dentistry, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland
5
Department of General Pathomorphology, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland
*
Authors to whom correspondence should be addressed.
†
The authors had equal participation.
Nutrients 2018, 10(9), 1178; https://doi.org/10.3390/nu10091178
Received: 29 July 2018 / Revised: 23 August 2018 / Accepted: 27 August 2018 / Published: 28 August 2018
(This article belongs to the Special Issue Impact of Bioactive Peptides on Human Health)
The aim of this study was to evaluate the effect of whey protein concentrate (WPC-80) on glycoconjugate catabolism, selected markers of oxidative stress and liver inflammation. The experiment was conducted on male Wistar rats (n = 63). The animals from the study group were administered WPC-80 at a dose of 0.3 or 0.5 g/kg body weight for 7, 14 or 21 days, while rats from the control group received only 0.9% NaCl. In liver homogenates, we assayed the activity of N-acetyl-β-D-hexosaminidase (HEX), β-glucuronidase (GLU), β-galactosidase (GAL), α-mannosidase (MAN), α-fucosidase (FUC), as well as the level of reduced glutathione (GSH), malondialdehyde (MDA), interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1). A significantly higher activity of HEX, GLU, MAN and FUC were found in the livers of rats receiving WPC-80 compared to controls. Serum ALT and AST were significantly higher in the animals supplemented with WPC-80 at a dose of 0.5 g/kg body weight for 21 days. In the same group of animals, enhanced level of GSH, MDA, IL-1β and TGF-β1 were also observed. WPC-80 is responsible for intensive remodelling of liver tissue and induction of oxidative stress especially at a dose of 0.5 g/kg body weight.
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Keywords:
exoglycosidases; liver; oxidative stress; whey
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MDPI and ACS Style
Żebrowska-Gamdzyk, M.; Maciejczyk, M.; Zalewska, A.; Guzińska-Ustymowicz, K.; Tokajuk, A.; Car, H. Whey Protein Concentrate WPC-80 Intensifies Glycoconjugate Catabolism and Induces Oxidative Stress in the Liver of Rats. Nutrients 2018, 10, 1178.
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