Next Article in Journal
Formula Milk Supplementation on the Postnatal Ward: A Cross-Sectional Analytical Study
Next Article in Special Issue
Protective Roles of Thymoquinone Nanoformulations: Potential Nanonutraceuticals in Human Diseases
Previous Article in Journal
Exercise and Nutrition Strategies to Counteract Sarcopenic Obesity
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Nutrients 2018, 10(5), 607; https://doi.org/10.3390/nu10050607

In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract

1
Faculty of Biotechnology, University of Agronomic Sciences and Veterinary Medicine, Bd. Mărăşti no. 59, district 1, 011464 Bucharest, Romania
2
Horia Hulubei National Institute for Physics and Nuclear Engineering, 30 Reactorului, 077125 Magurele, Romania
3
Faculty of Physics, University of Bucharest, 405 Atomistilor Blv., 077125 Magurele, Romania
4
ICUB-Research Institute of the University of Bucharest 36-46 Bd. M. Kogalniceanu, 5th District, 050107 Bucharest, Romania
*
Author to whom correspondence should be addressed.
Received: 12 April 2018 / Revised: 10 May 2018 / Accepted: 11 May 2018 / Published: 14 May 2018
Full-Text   |   PDF [973 KB, uploaded 14 May 2018]   |  

Abstract

The ability to orally administer silver nanoparticles (AgNPs) in enteric capsules implies a direct interaction with the colon microbiota. The in vitro effect provides a portrayal of the functional properties under in vivo conditions. The purpose of this study was to describe a green AgNP synthesis process, using aqueous extract from Lactarius piperatus mushroom, and to characterize the nanomaterial. We determined its antimicrobial and antioxidant effects in vitro in the microbiota of healthy individuals via the GIS1 system—a colon transit simulator. Per the quantitative polymerase chain reaction (qPCR) results, the antimicrobial properties of the AgNPs affected the initial share of different enteric species by decreasing the Bacteroides, Enterobacteriaceae, and Lactobacillus populations and favoring the Bifidobacterium group. The association between AgNPs and wild mushroom L. piperatus extract had a synergistic antibacterial activity against various pathogenic microorganisms while the mushroom extract reduced biofilm formation. Administration of AgNP maintained its constant antioxidant status, and it was correlated with a reduction in ammonium compounds. The physicochemical characterization of these NPs complemented their biochemical characterization. The maximum ultraviolet-visible spectroscopy (UV-VIS) absorbance was observed at 440 nm, while the Fourier transform infrared (FT-IR) spectrum reached a peak at 3296 cm–1, which was correlated with the high-performance liquid chromatographic analysis (HPLC). The major phenolic compound was homogentisic acid. The size (49 ± 16 nm in diameter) and spherical shape of the NPs were correlated with their biological effects in vitro. View Full-Text
Keywords: mushroom; scavenging activity; simulation; Bifidobacterium; homogentisic acid mushroom; scavenging activity; simulation; Bifidobacterium; homogentisic acid
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Vamanu, E.; Ene, M.; Biță, B.; Ionescu, C.; Crăciun, L.; Sârbu, I. In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract. Nutrients 2018, 10, 607.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top