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Open AccessArticle

In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract

Faculty of Biotechnology, University of Agronomic Sciences and Veterinary Medicine, Bd. Mărăşti no. 59, district 1, 011464 Bucharest, Romania
Horia Hulubei National Institute for Physics and Nuclear Engineering, 30 Reactorului, 077125 Magurele, Romania
Faculty of Physics, University of Bucharest, 405 Atomistilor Blv., 077125 Magurele, Romania
ICUB-Research Institute of the University of Bucharest 36-46 Bd. M. Kogalniceanu, 5th District, 050107 Bucharest, Romania
Author to whom correspondence should be addressed.
Nutrients 2018, 10(5), 607;
Received: 12 April 2018 / Revised: 10 May 2018 / Accepted: 11 May 2018 / Published: 14 May 2018
The ability to orally administer silver nanoparticles (AgNPs) in enteric capsules implies a direct interaction with the colon microbiota. The in vitro effect provides a portrayal of the functional properties under in vivo conditions. The purpose of this study was to describe a green AgNP synthesis process, using aqueous extract from Lactarius piperatus mushroom, and to characterize the nanomaterial. We determined its antimicrobial and antioxidant effects in vitro in the microbiota of healthy individuals via the GIS1 system—a colon transit simulator. Per the quantitative polymerase chain reaction (qPCR) results, the antimicrobial properties of the AgNPs affected the initial share of different enteric species by decreasing the Bacteroides, Enterobacteriaceae, and Lactobacillus populations and favoring the Bifidobacterium group. The association between AgNPs and wild mushroom L. piperatus extract had a synergistic antibacterial activity against various pathogenic microorganisms while the mushroom extract reduced biofilm formation. Administration of AgNP maintained its constant antioxidant status, and it was correlated with a reduction in ammonium compounds. The physicochemical characterization of these NPs complemented their biochemical characterization. The maximum ultraviolet-visible spectroscopy (UV-VIS) absorbance was observed at 440 nm, while the Fourier transform infrared (FT-IR) spectrum reached a peak at 3296 cm–1, which was correlated with the high-performance liquid chromatographic analysis (HPLC). The major phenolic compound was homogentisic acid. The size (49 ± 16 nm in diameter) and spherical shape of the NPs were correlated with their biological effects in vitro. View Full-Text
Keywords: mushroom; scavenging activity; simulation; Bifidobacterium; homogentisic acid mushroom; scavenging activity; simulation; Bifidobacterium; homogentisic acid
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Vamanu, E.; Ene, M.; Biță, B.; Ionescu, C.; Crăciun, L.; Sârbu, I. In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract. Nutrients 2018, 10, 607.

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