Next Article in Journal
Galacto-Oligosaccharide/Polidextrose Enriched Formula Protects against Respiratory Infections in Infants at High Risk of Atopy: A Randomized Clinical Trial
Previous Article in Journal
Sodium Content of Lunches and Snacks Provided in Australian Long Day Care Centres: A Cross-Sectional Study
Open AccessArticle

The Postprandial Anti-Hyperglycemic Effect of Pyridoxine and Its Derivatives Using In Vitro and In Vivo Animal Models

1
Department of Food and Animal Biotechnology, Seoul National University, 08826 Seoul, Korea
2
Department of Food and Nutrition, Hannam University, 34049 Daejeon, Korea
3
Department of Bio Quality Control, Korea Bio Polytechnic, 32943 Chungnam, Korea
4
Department of Chemistry and Food Science, Framingham State University, Framingham, MA 01701, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2018, 10(3), 285; https://doi.org/10.3390/nu10030285
Received: 17 January 2018 / Revised: 20 February 2018 / Accepted: 27 February 2018 / Published: 28 February 2018
In the current study, we investigated the inhibitory activity of pyridoxine, pyridoxal, and pyridoxamine, against various digestive enzymes such as α-glucosidases, sucrase, maltase, and glucoamylase. Inhibition of these enzymes involved in the absorption of disaccharide can improve post-prandial hyperglycemia due to a carbohydrate-based diet. Pyridoxal (4.14 mg/mL of IC50) had the highest rat intestinal α-glucosidase inhibitory activity, followed by pyridoxamine and pyridoxine (4.85 and 5.02 mg/mL of IC50, respectively). Pyridoxal demonstrated superior inhibition against maltase (0.38 mg/mL IC50) and glucoamylase (0.27 mg/mLIC50). In addition, pyridoxal showed significant higher α-amylase inhibitory activity (10.87 mg/mL of IC50) than that of pyridoxine (23.18 mg/mL of IC50). This indicates that pyridoxal can also inhibit starch hydrolyzing by pancreatic α-amylase in small intestine. Based on these in vitro results, the deeper evaluation of the anti-hyperglycemic potential of pyridoxine and its derivatives using Sprague-Dawley (SD) rat models, was initiated. The post-prandial blood glucose levels were tested two hours after sucrose/starch administration, with and without pyridoxine and its derivatives. In the animal trial, pyridoxal (p < 0.05) had a significantly reduction to the postprandial glucose levels, when compared to the control. The maximum blood glucose levels (Cmax) of pyridoxal administration group were decreased by about 18% (from 199.52 ± 22.93 to 164.10 ± 10.27, p < 0.05) and 19% (from 216.92 ± 12.46 to 175.36 ± 10.84, p < 0.05) in sucrose and starch loading tests, respectively, when compared to the control in pharmacodynamics study. The pyridoxal administration significantly decreased the minimum, maximum, and mean level of post-prandial blood glucose at 0.5 h after meals. These results indicate that water-soluble vitamin pyridoxine and its derivatives can decrease blood glucose level via the inhibition of carbohydrate-hydrolyzing and absorption-linked enzymes. Therefore, pyridoxal may have the potential to be used as a food ingredient for the prevention of prediabetes progression to type 2 diabetes. View Full-Text
Keywords: pyridoxine; anti-hyperglycemia; postprandial; α-glucosidase; inhibition pyridoxine; anti-hyperglycemia; postprandial; α-glucosidase; inhibition
Show Figures

Figure 1

MDPI and ACS Style

Kim, H.H.; Kang, Y.-R.; Lee, J.-Y.; Chang, H.-B.; Lee, K.W.; Apostolidis, E.; Kwon, Y.-I. The Postprandial Anti-Hyperglycemic Effect of Pyridoxine and Its Derivatives Using In Vitro and In Vivo Animal Models. Nutrients 2018, 10, 285. https://doi.org/10.3390/nu10030285

AMA Style

Kim HH, Kang Y-R, Lee J-Y, Chang H-B, Lee KW, Apostolidis E, Kwon Y-I. The Postprandial Anti-Hyperglycemic Effect of Pyridoxine and Its Derivatives Using In Vitro and In Vivo Animal Models. Nutrients. 2018; 10(3):285. https://doi.org/10.3390/nu10030285

Chicago/Turabian Style

Kim, Hyuk Hwa; Kang, Yu-Ri; Lee, Jung-Yun; Chang, Hung-Bae; Lee, Ki Won; Apostolidis, Emmanouil; Kwon, Young-In. 2018. "The Postprandial Anti-Hyperglycemic Effect of Pyridoxine and Its Derivatives Using In Vitro and In Vivo Animal Models" Nutrients 10, no. 3: 285. https://doi.org/10.3390/nu10030285

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop