Differences in Tissue Distribution of Cyano–B12 and Hydroxo–B12 One Week after Oral Intake: An Experimental Study in Male Wistar Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Study Design
2.3. Diets with Low and High B12 Content
2.4. Solutions for Oral Administration
2.5. Quantification of Endogenous B12 in Plasma and Tissues
2.6. Quantification of [57Co]B12 Accumulation in Plasma and Tissues
2.7. Statistical Methods
3. Results
3.1. B12 Content and Forms in the Diet
3.2. Endogenous B12 Distribution in B12-Deplete and B12-Replete Rats
3.3. Distribution of [57Co]B12 One Week after Oral Administration to B12-Deplete and B12-Replete Rats
4. Discussion
4.1. Introduction of B12 Depletion
4.2. Distribution of Labeled CN–B12 and HO–B12
4.3. Tissues/Plasma Ratios for CN–B12 and HO–B12
4.4. Thoughts on the Isotope Dilution Technique
4.5. Study Limitations
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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B12-Deplete Rats (n = 16) | B12-Replete Rats (n = 12) | |
---|---|---|
Liver (pmol/g) | 28.2 (23.4–41.1) | 33.2 (25.2–46.7) |
Kidneys (pmol/g) | 123 (104–188) | 1300 (1133–1512) |
Plasma (pmol/L) | 565 (353–646) | 1410 (1150–1630) |
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Greibe, E.; Nymark, O.; Fedosov, S.N.; Heegaard, C.W.; Nexo, E. Differences in Tissue Distribution of Cyano–B12 and Hydroxo–B12 One Week after Oral Intake: An Experimental Study in Male Wistar Rats. Nutrients 2018, 10, 1487. https://doi.org/10.3390/nu10101487
Greibe E, Nymark O, Fedosov SN, Heegaard CW, Nexo E. Differences in Tissue Distribution of Cyano–B12 and Hydroxo–B12 One Week after Oral Intake: An Experimental Study in Male Wistar Rats. Nutrients. 2018; 10(10):1487. https://doi.org/10.3390/nu10101487
Chicago/Turabian StyleGreibe, Eva, Ole Nymark, Sergey N. Fedosov, Christian W. Heegaard, and Ebba Nexo. 2018. "Differences in Tissue Distribution of Cyano–B12 and Hydroxo–B12 One Week after Oral Intake: An Experimental Study in Male Wistar Rats" Nutrients 10, no. 10: 1487. https://doi.org/10.3390/nu10101487