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Nutrients 2018, 10(10), 1481; https://doi.org/10.3390/nu10101481

Microbiota and Derived Parameters in Fecal Samples of Infants with Non-IgE Cow’s Milk Protein Allergy under a Restricted Diet

1
Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA)-Consejo Superior de Investigaciones Científicas (CSIC), 33300 Villaviciosa, Spain
2
Department of Nutrition and Food Science, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain
3
Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43121 Parma, Italy
4
Pediatric Gastroenterology and Nutrition Section, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain
5
Pediatrics, Hospital Universitario de Cabueñes, 33394 Gijón, Spain
6
Pediatrics, Hospital Universitario San Agustín, 33401 Avilés, Spain
These authors contribute equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 21 September 2018 / Revised: 5 October 2018 / Accepted: 6 October 2018 / Published: 11 October 2018
(This article belongs to the Special Issue Probiotics and Prebiotics in Pediatrics)
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Abstract

Cow’s milk protein allergy (CMPA) is the most common food allergy in infancy. Non-IgE mediated (NIM) forms are little studied and the responsible mechanisms of tolerance acquisition remain obscure. Our aim was to study the intestinal microbiota and related parameters in the fecal samples of infants with NIM-CMPA, to establish potential links between type of formula substitutes, microbiota, and desensitization. Seventeen infants between one and two years old, diagnosed with NIM-CMPA, were recruited. They were all on an exclusion diet for six months, consuming different therapeutic protein hydrolysates. After this period, stool samples were obtained and tolerance development was evaluated by oral challenges. A control group of 10 age-matched healthy infants on an unrestricted diet were included in the study. Microbiota composition, short-chain fatty acids, calprotectin, and transforming growth factor (TGF)-β1 levels were determined in fecal samples from both groups. Infants with NIM-CMPA that consumed vegetable protein-based formulas presented microbiota colonization patterns different from those fed with an extensively hydrolyzed formula. Differences in microbiota composition and fecal parameters between NIM-CMPA and healthy infants were observed. Non-allergic infants showed a significantly higher proportion of Bacteroides compared to infants with NIM-CMPA. The type of protein hydrolysate was found to determine gut microbiota colonization and influence food allergy resolution in NIM-CMPA cases. View Full-Text
Keywords: fecal microbiota; protein hydrolyzed formulas; cow’s milk protein; tolerance acquisition; non-IgE mediated allergy fecal microbiota; protein hydrolyzed formulas; cow’s milk protein; tolerance acquisition; non-IgE mediated allergy
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Díaz, M.; Guadamuro, L.; Espinosa-Martos, I.; Mancabelli, L.; Jiménez, S.; Molinos-Norniella, C.; Pérez-Solis, D.; Milani, C.; Rodríguez, J.M.; Ventura, M.; Bousoño, C.; Gueimonde, M.; Margolles, A.; Díaz, J.J.; Delgado, S. Microbiota and Derived Parameters in Fecal Samples of Infants with Non-IgE Cow’s Milk Protein Allergy under a Restricted Diet. Nutrients 2018, 10, 1481.

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