Glycaemic Index of Maternal Dietary Carbohydrate Differentially Alters Fto and Lep Expression in Offspring in C57BL/6 Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Diets
2.2. In Vitro GI Testing of the Diets
2.3. Animals
2.4. Food Intake and Weight Monitoring
2.5. Glucose and Insulin Tolerance Tests
2.6. Mouse Sacrifice, Blood and Tissue Collection
2.7. Quantitative Real-Time PCR (qRT-PCR)
2.8. Plasma Analysis
2.9. Statistical Analysis
3. Results
3.1. Rate of Starch Digestion in Vitro
3.2. Food Intake and Body Weight
3.3. Glucose and Insulin Tolerance Tests
3.4. Fto Gene Expression
3.5. Leptin Gene Expression
3.6. Hypothalamic Appetite Gene Expression
3.7. Plasma Hormones
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sideratou, T.; Atkinson, F.; Campbell, G.J.; Petocz, P.; Bell-Anderson, K.S.; Brand-Miller, J. Glycaemic Index of Maternal Dietary Carbohydrate Differentially Alters Fto and Lep Expression in Offspring in C57BL/6 Mice. Nutrients 2018, 10, 1342. https://doi.org/10.3390/nu10101342
Sideratou T, Atkinson F, Campbell GJ, Petocz P, Bell-Anderson KS, Brand-Miller J. Glycaemic Index of Maternal Dietary Carbohydrate Differentially Alters Fto and Lep Expression in Offspring in C57BL/6 Mice. Nutrients. 2018; 10(10):1342. https://doi.org/10.3390/nu10101342
Chicago/Turabian StyleSideratou, Theodora, Fiona Atkinson, Grace J. Campbell, Peter Petocz, Kim S. Bell-Anderson, and Jennie Brand-Miller. 2018. "Glycaemic Index of Maternal Dietary Carbohydrate Differentially Alters Fto and Lep Expression in Offspring in C57BL/6 Mice" Nutrients 10, no. 10: 1342. https://doi.org/10.3390/nu10101342