Towards a Sustainable and Ethical Integration of AI Chatbots in Higher Education
Abstract
1. Introduction
2. Theoretical Framework
2.1. Background to AI Integration in Universities
2.2. AI Literacy in Education. Changing Roles in Educational Activities
2.3. AI Chatbots as Educational Tools
2.4. Students’ Perspective on AI Chatbots
2.5. Risks and Limitations of AI Chatbots
2.6. Longitudinal Studies on the Long-Term Impact of Artificial Intelligence Chatbots on Students’ Educational Experiences
3. Materials and Methods
4. Results
5. Discussion
6. Conclusions
6.1. Theoretical and Practical Implications for Higher Education Institutions
6.2. Limitations of the Paper and Future Research Directions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
| AI | Artificial Intelligence |
| AI Chatbots | Artificial Intelligence Chatbots |
| AII | Artificial Intelligence Integration in universities |
| AIL | Artificial Intelligence Literacy |
| AIR | Academic Integrity Risks |
| AIU | AI Chatbots are used for assisted and personalized student learning |
| BEN | Cognitive and pedagogical benefits of AI Chatbots |
| FT | Features of AI Chatbots |
| LAR | AI Chatbots’ limitations of accuracy and reliability. |
| PR | Professors’ perceptions of the use of AI Chatbots in universities |
| RL | Cognitive risks and limitations of AI Chatbots |
| ST | Positive perceptions and intention to use AI Chatbots among students |
Appendix A
| Construct | Item | Scale | Scale Reference |
|---|---|---|---|
| AII | AII1 | Universities must design explicit procedures, approaches, and frameworks for the trustworthy integration of AI Chatbots. These include managing intellectual integrity, data privacy, and algorithmic bias. | [32,34,37,38] |
| AII2 | Ample empirical research is required to comprehend the real influence of AI Chatbots on education. | [27,32,37,63,64,75] | |
| AII3 | Longitudinal studies can provide a more detailed understanding of the long-term impact and evolution of student experiences with AI Chatbots. | [64,72] | |
| AII4 | Teachers must redefine their roles, focusing on critical pedagogical decisions, encouraging students to be active investigators, and raising ethical awareness. | [55] | |
| AII5 | The design of assessments should focus on the application and interpretation of knowledge, not just basic knowledge, to prevent unauthorized use and encourage critical thinking. | [45,62] | |
| AII6 | AI Chatbot developers should focus on intuitive design, performance improvement, and integrating features that facilitate knowledge sharing. | [64] | |
| AIL | AIL1 | Providing training sessions and resources to familiarize students and teachers with AI Chatbots and their functionalities. | [34,38,50,64,73] |
| AIL2 | Training should focus on building confidence in using the tool and addressing technical barriers. | [66] | |
| AIL3 | Educating students on AI Chatbots’ capabilities, ethical implications, and limits, encouraging their responsible use. | [37,38,64] | |
| AIL4 | Users should always check the information AI Chatbots provide, as it is unreliable. | [25,37,38,45,57,62,65,81] | |
| AIL5 | Encouraging interdisciplinary collaboration to fully harness the benefits of AI Chatbots in an educational context. | [34,37] | |
| PR | PR1 | The general perception of university professors is positive regarding implementing AI Chatbots in teaching activities. | [27] |
| PR2 | University professors see it as a means to support time-consuming teaching activities, stimulate interest, activate and engage learners, and stimulate their critical thinking and creativity. | [27] | |
| PR3 | Most university professors consider studying AI Chatbots for proper use in education. | [27] | |
| PR4 | The professors recognise the potential of AI Chatbots as a tool to support and improve traditional methods, but emphasise the importance of complementing, not replacing, human interaction. | [54,83] | |
| FT | FT1 | AI Chatbots can assist in academic research, courses, assignments, and projects. | [31,37] |
| FT2 | AI Chatbots can summarise information and analyse content. | [31,64,65,81] | |
| FT3 | AI Chatbots provide initial essay ideas and can help with the research writing. | [37] | |
| FT4 | Users can use AI Chatbots for topic exploration, brainstorming, and schema development. | [45] | |
| FT5 | AI Chatbots can generate linguistic and discourse analysis. | [66] | |
| FT6 | AI Chatbots are effective at composing explanatory answers and can write manuscripts. | [32,81] | |
| BEN | BEN1 | Its ability to provide instant answers can stimulate critical thinking. | [31] |
| BEN2 | Through AI-generated text analysis, users can learn to evaluate information and develop critical thinking skills. | [39,45,53] | |
| BEN3 | The use of AI Chatbots exposed different points of view, encouraging the user to think about questions other than those initially proposed by the user (i.e., it expanded the conversation). | [3] | |
| ST | ST1 | Students are familiar with AI Chatbots. | [65] |
| ST2 | Students perceive AI Chatbots as especially useful for generating new ideas, saving time, and improving efficiency. | [56,65,81] | |
| ST3 | Higher education students’ intent to use AI Chatbots is influenced by their perceived ease of use, usefulness, interactivity, and personalisation. | [64,72] | |
| AIU | AIU1 | Students can use AI Chatbots as a study tool, complementary to traditional learning, and as a revision aid in exam preparation. | [49,82] |
| AIU2 | They can ask AI Chatbots to explain their answers and generate quiz questions. | [49] | |
| AIU3 | AI Chatbots can provide personalised learning experiences by tailoring content to students’ needs. | [31,65] | |
| AIU4 | AI Chatbots can provide tailored feedback and explanations, helping students understand complex concepts and identify knowledge gaps. | [34,37,38,110] | |
| AIU5 | AI Chatbots are patient virtual tutors available 24/7. They explain concepts in different ways and provide instant access to information and resources. | [37,65,81,83] | |
| RL | RL1 | The danger of becoming overly dependent on AI Chatbots can reduce students’ critical thinking, problem-solving, and creativity skills. | [28,37,38,40,45,53,65,67] |
| RL2 | AI Chatbots could lead to poor user training if a process of deepening and nuancing understanding is missing. | [50,67] | |
| RL3 | Some experts believe that the extensive use of AI Chatbots could decrease competence in creative thinking. | [81] | |
| RL4 | AI Chatbots do not possess the same level of human interaction and interpersonal skills as instructors or peers, limiting their effectiveness in group activities and collaborative discussions. | [37,38,83] | |
| RL5 | New skills and thinking styles are needed to harness AI Chatbots’ potential fully. | [63] | |
| RL6 | Some teachers fear that students could become lazy and rely on AI Chatbots to avoid the human effort required to acquire intellectual skills. | [25,32,57,65] | |
| AIR | AIR1 | One of the biggest concerns is the risk of plagiarism and cheating, as students can use AI Chatbots to generate content for assignments or exams, which can bypass conventional plagiarism detectors. | [27,32,37,40,45,53,60,61,62,63,64,65,84,111] |
| AIR2 | A struggling student can substantially improve their score by using AI Chatbots unauthorisedly. | [49] | |
| AIR3 | Cheating occurs when students purchase third-party writing services. | [64] | |
| AIR4 | The lack of attribution of sources by AI Chatbots raises ethical and learning issues. | [25] | |
| LAR | LAR1 | AI Chatbots can generate incorrect, misleading, or biased answers because they lack a deep understanding of the meaning of words and rely on statistical models. They can even produce artificial hallucinations, generating information that does not exist, including false references. | [27,37,45,53,63,64,65,81,82] |
| LAR2 | AI Chatbots’ knowledge is limited to the data they have been trained on, so they cannot provide real-time information or information about recent events. | [25,27,37,45,65,84] | |
| LAR3 | The answers can be vague, without providing sufficient details or specific information. | [65] | |
| LAR4 | It is difficult to assess the quality of the answers and the credibility of the data on which they have been trained. | [53,65,67] |
Appendix B
| Mean | Median | Min | Max | Standard Deviation | Excess Kurtosis | Skewness | |
|---|---|---|---|---|---|---|---|
| AII1 | 4.414 | 5.000 | 2.000 | 5.000 | 0.839 | −0.534 | −1.003 |
| AII2 | 4.331 | 5.000 | 2.000 | 5.000 | 0.968 | −1.006 | −0.866 |
| AII3 | 4.392 | 5.000 | 3.000 | 5.000 | 0.862 | −1.119 | −0.850 |
| AII4 | 4.373 | 5.000 | 1.000 | 5.000 | 0.957 | −0.293 | −1.075 |
| AII5 | 4.343 | 5.000 | 2.000 | 5.000 | 0.923 | −1.292 | −0.770 |
| AII6 | 4.461 | 5.000 | 2.000 | 5.000 | 0.907 | −0.296 | −1.200 |
| AIL1 | 4.279 | 5.000 | 1.000 | 5.000 | 0.980 | −0.478 | −0.928 |
| AIL2 | 4.267 | 5.000 | 1.000 | 5.000 | 0.978 | −0.268 | −0.936 |
| AIL3 | 4.353 | 5.000 | 1.000 | 5.000 | 0.853 | 0.539 | −1.126 |
| AIL4 | 4.461 | 5.000 | 2.000 | 5.000 | 0.912 | −0.162 | −1.236 |
| AIL5 | 4.350 | 5.000 | 1.000 | 5.000 | 0.940 | −0.264 | −1.035 |
| PR1 | 3.949 | 4.000 | 1.000 | 5.000 | 1.036 | −1.012 | −0.414 |
| PR2 | 3.988 | 4.000 | 1.000 | 5.000 | 1.004 | −0.750 | −0.472 |
| PR3 | 4.213 | 5.000 | 2.000 | 5.000 | 0.921 | −1.040 | −0.643 |
| PR4 | 4.402 | 5.000 | 1.000 | 5.000 | 0.886 | 0.004 | −1.113 |
| FT1 | 4.598 | 5.000 | 1.000 | 5.000 | 0.883 | 2.478 | −1.951 |
| FT2 | 4.853 | 5.000 | 1.000 | 5.000 | 0.601 | 20.383 | −4.426 |
| FT3 | 4.716 | 5.000 | 2.000 | 5.000 | 0.746 | 4.276 | −2.400 |
| FT4 | 4.794 | 5.000 | 2.000 | 5.000 | 0.616 | 5.901 | −2.749 |
| FT5 | 4.613 | 5.000 | 1.000 | 5.000 | 0.847 | 2.473 | −1.935 |
| FT6 | 4.598 | 5.000 | 2.000 | 5.000 | 0.777 | 1.419 | −1.685 |
| BEN1 | 4.142 | 5.000 | 1.000 | 5.000 | 1.188 | −0.490 | −0.946 |
| BEN2 | 4.338 | 5.000 | 1.000 | 5.000 | 1.079 | 0.282 | −1.280 |
| BEN3 | 4.571 | 5.000 | 1.000 | 5.000 | 0.874 | 1.701 | −1.742 |
| ST1 | 4.701 | 5.000 | 3.000 | 5.000 | 0.645 | 2.201 | −1.938 |
| ST2 | 4.632 | 5.000 | 3.000 | 5.000 | 0.739 | 0.835 | −1.633 |
| ST3 | 4.324 | 5.000 | 1.000 | 5.000 | 0.969 | −0.677 | −0.913 |
| AIU1 | 4.779 | 5.000 | 1.000 | 5.000 | 0.690 | 10.770 | −3.276 |
| AIU2 | 4.721 | 5.000 | 1.000 | 5.000 | 0.789 | 7.879 | −2.883 |
| AIU3 | 4.735 | 5.000 | 1.000 | 5.000 | 0.740 | 7.519 | −2.811 |
| AIU4 | 4.789 | 5.000 | 1.000 | 5.000 | 0.693 | 11.567 | −3.419 |
| AIU5 | 4.691 | 5.000 | 1.000 | 5.000 | 0.862 | 7.609 | −2.863 |
| RL1 | 4.407 | 5.000 | 1.000 | 5.000 | 1.032 | 1.114 | −1.495 |
| RL2 | 4.402 | 5.000 | 1.000 | 5.000 | 0.998 | 0.770 | −1.378 |
| RL3 | 4.309 | 5.000 | 1.000 | 5.000 | 1.051 | 0.071 | −1.151 |
| RL4 | 4.309 | 5.000 | 1.000 | 5.000 | 1.040 | 0.282 | −1.170 |
| RL5 | 4.314 | 5.000 | 2.000 | 5.000 | 0.923 | −0.971 | −0.812 |
| RL6 | 4.309 | 5.000 | 1.000 | 5.000 | 0.979 | −0.154 | −1.060 |
| AIR1 | 4.108 | 5.000 | 1.000 | 5.000 | 1.187 | −0.492 | −0.897 |
| AIR2 | 4.020 | 5.000 | 1.000 | 5.000 | 1.163 | −1.013 | −0.611 |
| AIR3 | 3.868 | 4.000 | 1.000 | 5.000 | 1.199 | −1.057 | −0.445 |
| AIR4 | 4.147 | 5.000 | 1.000 | 5.000 | 1.152 | −0.190 | −0.966 |
| LAR1 | 4.333 | 5.000 | 2.000 | 5.000 | 0.993 | −0.703 | −0.978 |
| LAR2 | 3.951 | 5.000 | 1.000 | 5.000 | 1.218 | −1.061 | −0.576 |
| LAR3 | 3.936 | 5.000 | 1.000 | 5.000 | 1.274 | −1.059 | −0.629 |
| LAR4 | 4.042 | 5.000 | 1.000 | 5.000 | 1.134 | −1.298 | −0.528 |
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| Characteristics | Category | Frequency | % |
|---|---|---|---|
| Gender | Female | 228 | 55.88 |
| Male | 180 | 44.12 | |
| Students | First-year | 90 | 22.06 |
| Second-year | 140 | 34.31 | |
| Third year | 178 | 43.63 | |
| Age | 18 | 8 | 1.96 |
| 19 | 85 | 20.83 | |
| 20 | 123 | 30.15 | |
| 21 | 144 | 35.29 | |
| 22 | 33 | 8.09 | |
| 23 | 7 | 1.72 | |
| over 23 | 8 | 1.96 | |
| AI chatbot using | ChatGPT | 408 | 100.00 |
| Gemini | 74 | 18.14 | |
| DeepSeek | 46 | 11.27 | |
| Microsoft Copilot | 40 | 9.80 | |
| Claude | 20 | 4.90 | |
| Grok | 14 | 3.43 | |
| Perplexity | 8 | 1.96 |
| Construct | Item | Outer Loading | Cronbach’s Alpha | rho_a | CR | AVE |
|---|---|---|---|---|---|---|
| AII | AII1 | 0.802 | 0.849 | 0.850 | 0.888 | 0.570 |
| AII2 | 0.743 | |||||
| AII3 | 0.769 | |||||
| AII4 | 0.700 | |||||
| AII5 | 0.754 | |||||
| AII6 | 0.759 | |||||
| AIL | AIL1 | 0.775 | 0.819 | 0.819 | 0.873 | 0.580 |
| AIL2 | 0.801 | |||||
| AIL3 | 0.800 | |||||
| AIL4 | 0.687 | |||||
| AIL5 | 0.738 | |||||
| AIR1 | 0.775 | 0.830 | 0.835 | 0.887 | 0.662 | |
| AIR2 | 0.821 | |||||
| AIR3 | 0.841 | |||||
| AIR4 | 0.816 | |||||
| AIU1 | 0.884 | 0.835 | 0.862 | 0.883 | 0.604 | |
| AIU2 | 0.722 | |||||
| AIU3 | 0.704 | |||||
| AIU4 | 0.825 | |||||
| AIU5 | 0.735 | |||||
| BEN1 | 0.810 | 0.743 | 0.746 | 0.854 | 0.662 | |
| BEN2 | 0.866 | |||||
| BEN3 | 0.762 | |||||
| FT1 | 0.769 | 0.848 | 0.852 | 0.887 | 0.567 | |
| FT2 | 0.732 | |||||
| FT3 | 0.783 | |||||
| FT4 | 0.780 | |||||
| FT5 | 0.726 | |||||
| FT6 | 0.726 | |||||
| LAR1 | 0.782 | 0.765 | 0.769 | 0.850 | 0.587 | |
| LAR2 | 0.733 | |||||
| LAR3 | 0.736 | |||||
| LAR4 | 0.810 | |||||
| PR1 | 0.702 | 0.713 | 0.716 | 0.822 | 0.536 | |
| PR2 | 0.792 | |||||
| PR3 | 0.713 | |||||
| PR4 | 0.719 | |||||
| RL1 | 0.772 | 0.875 | 0.876 | 0.905 | 0.615 | |
| RL2 | 0.809 | |||||
| RL3 | 0.794 | |||||
| RL4 | 0.796 | |||||
| RL5 | 0.785 | |||||
| RL6 | 0.747 | |||||
| ST1 | 0.896 | 0.786 | 0.800 | 0.877 | 0.705 | |
| ST2 | 0.890 | |||||
| ST3 | 0.722 |
| AII | AlL | AlR | AlU | BEN | FT | LAR | PR | RL | ST | |
|---|---|---|---|---|---|---|---|---|---|---|
| AII | 0.755 | |||||||||
| AlL | 0.703 | 0.761 | ||||||||
| AlR | 0.465 | 0.351 | 0.813 | |||||||
| AlU | 0.305 | 0.269 | 0.269 | 0.777 | ||||||
| BEN | 0.283 | 0.298 | 0.168 | 0.433 | 0.814 | |||||
| FT | 0.431 | 0.389 | 0.205 | 0.543 | 0.517 | 0.753 | ||||
| LAR | 0.362 | 0.320 | 0.546 | 0.148 | 0.158 | 0.234 | 0.766 | |||
| PR | 0.642 | 0.529 | 0.364 | 0.371 | 0.403 | 0.384 | 0.278 | 0.732 | ||
| RL | 0.572 | 0.446 | 0.633 | 0.240 | 0.124 | 0.270 | 0.495 | 0.384 | 0.784 | |
| ST | 0.674 | 0.600 | 0.402 | 0.472 | 0.253 | 0.513 | 0.235 | 0.562 | 0.567 | 0.840 |
| AII | AlL | AlR | AlU | BEN | FT | LAR | PR | RL | ST | |
|---|---|---|---|---|---|---|---|---|---|---|
| AII | ||||||||||
| AlL | 0.822 | |||||||||
| AlR | 0.548 | 0.419 | ||||||||
| AlU | 0.353 | 0.312 | 0.314 | |||||||
| BEN | 0.356 | 0.380 | 0.213 | 0.547 | ||||||
| FT | 0.495 | 0.462 | 0.242 | 0.626 | 0.650 | |||||
| LAR | 0.447 | 0.398 | 0.686 | 0.179 | 0.232 | 0.283 | ||||
| PR | 0.812 | 0.668 | 0.464 | 0.463 | 0.558 | 0.470 | 0.377 | |||
| RL | 0.661 | 0.517 | 0.745 | 0.265 | 0.203 | 0.308 | 0.608 | 0.473 | ||
| ST | 0.821 | 0.730 | 0.492 | 0.567 | 0.329 | 0.620 | 0.306 | 0.728 | 0.677 |
| D | I | T | D | T | D | T | |
|---|---|---|---|---|---|---|---|
| AII | AII | AII | PR | PR | ST | ST | |
| AII | 0.348 | 0.348 | |||||
| AIR | 0.056 | 0.056 | |||||
| AlU | 0.097 | 0.097 | 0.472 | 0.472 | |||
| BEN | 0.071 | 0.071 | 0.278 | 0.278 | |||
| FT | 0.062 | 0.062 | 0.240 | 0.240 | |||
| LAR | 0.024 | 0.024 | |||||
| PR | 0.256 | 0.256 | |||||
| RL | 0.154 | 0.154 | |||||
| ST | 0.205 | 0.205 |
| Path Coefficients | 0.025 | 0.975 | T Statistics | p Values | Remark | |
|---|---|---|---|---|---|---|
| AlL -> AII | 0.348 | 0.269 | 0.430 | 8.495 | 0.000 | H1 is supported |
| PR -> AII | 0.256 | 0.162 | 0.356 | 5.191 | 0.000 | H2 is supported |
| FT -> PR | 0.240 | 0.156 | 0.338 | 5.095 | 0.000 | H3 is supported |
| BEN -> PR | 0.278 | 0.172 | 0.378 | 5.289 | 0.000 | H4 is supported |
| ST -> AII | 0.205 | 0.112 | 0.290 | 4.487 | 0.000 | H5 is supported |
| AlU -> ST | 0.472 | 0.375 | 0.572 | 9.493 | 0.000 | H6 is supported |
| RL -> AII | 0.154 | 0.068 | 0.241 | 3.511 | 0.000 | H7 is supported |
| AlR -> AII | 0.056 | −0.024 | 0.139 | 1.334 | 0.182 | H8 is not supported |
| LAR -> AII | 0.024 | −0.065 | 0.115 | 0.524 | 0.601 | H9 is not supported |
| Construct | Full Collinearity VIF |
|---|---|
| AII | 2.636 |
| AIL | 2.649 |
| PR | 2.723 |
| ST | 2.908 |
| BEN | 2.869 |
| FT | 2.873 |
| AIU | 2.967 |
| RL | 2.911 |
| AIR | 2.931 |
| LAR | 3.007 |
| AII | PR | ST | |
|---|---|---|---|
| AlL | 0.206 | ||
| AlR | 0.005 | ||
| AlU | 0.287 | ||
| BEN | 0.071 | ||
| FT | 0.053 | ||
| LAR | 0.001 | ||
| PR | 0.122 | ||
| RL | 0.032 | ||
| ST | 0.059 |
| Dimension | Hypotheses | Description | Theoretical Grounding | Observation |
|---|---|---|---|---|
| Cognitive and Perceptual Drivers | ||||
| Competence Dimension | H1—GenAI literacy | cognitive capability, critical AI understanding, and informed evaluation capacity. | Knowledge-based view [101]; IS capability frameworks [93]; Digital competence theory [102]. | This hypothesis suggests that the legitimacy of AI integration is partially rooted in informed literacy rather than blind enthusiasm. |
| Teachers’ Perceptual Dimension | H2—Teachers’ favorable perceptions H3—AI Chatbot characteristics H4—Cognitive and pedagogical benefits | These hypotheses establish a structural chain: AI Chatbot characteristics → Teacher perception → Normative support | TAM (Perceived Usefulness) [91] UTAUT (Performance Expectancy) [92] IS Success Model [93] | Unlike traditional adoption studies, the outcome is not behavioral intention, but institutional legitimacy perception. |
| Students’ Perceptual Dimension | H5—Students’ positive perceptions H6—AI-supported personalized learning | These hypotheses establish a structural chain: AI-assisted learning → Student perception → Normative support | Student-centered learning theory [103], Educational technology acceptance models [92], Diffusion of innovation logic [104] | By incorporating both teachers and students, the model captures institutional legitimacy as a socially distributed construct. |
| Ethical and Cognitive Inhibitors—the model includes both drivers and barriers. | ||||
| H7—Cognitive risks | deskilling, overreliance, superficial learning | Risk–benefit adoption frameworks [105] Technology resistance models [106], Technology trust theory [107], Institutional legitimacy theory [96] | ||
| H8—Academic integrity risks | plagiarism, ghostwriting, assessment validity erosion | |||
| H9—Accuracy and reliability limitations | Hallucinations, factual inaccuracies, instability | |||
| Central Institutional Mechanism: Perceived Normative Support | ||||
| The pivotal construct of the model is: Perceived Normative Support for Institutional AI Integration | It functions as a legitimacy mechanism, a perceived normative climate, and a social filter for technological adoption. | Theory of Planned Behavior (subjective norm component) [108], Institutional theory (normative pressures) [95], Organizational climate theory [108], Legitimacy theory [109]. | By positioning normative support as central, the model extends beyond TAM/UTAUT and moves into the domain of Institutional AI governance and organizational transformation. | |
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Share and Cite
Voicu, M.-C.; Sîrghi, N.; Mircea, G.; Toth, D.M.-M. Towards a Sustainable and Ethical Integration of AI Chatbots in Higher Education. Sustainability 2026, 18, 2534. https://doi.org/10.3390/su18052534
Voicu M-C, Sîrghi N, Mircea G, Toth DM-M. Towards a Sustainable and Ethical Integration of AI Chatbots in Higher Education. Sustainability. 2026; 18(5):2534. https://doi.org/10.3390/su18052534
Chicago/Turabian StyleVoicu, Mirela-Catrinel, Nicoleta Sîrghi, Gabriela Mircea, and Daniela Maria-Magdalena Toth. 2026. "Towards a Sustainable and Ethical Integration of AI Chatbots in Higher Education" Sustainability 18, no. 5: 2534. https://doi.org/10.3390/su18052534
APA StyleVoicu, M.-C., Sîrghi, N., Mircea, G., & Toth, D. M.-M. (2026). Towards a Sustainable and Ethical Integration of AI Chatbots in Higher Education. Sustainability, 18(5), 2534. https://doi.org/10.3390/su18052534

