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Sustainability
Volume 17
Issue 22
10.3390/su172210082
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Sustainable Production of Added-Value Metabolic Compounds Under Adverse Culture Conditions by Microorganisms: A Case Study of Yarrowia lipolytica Strain Cultivated on Agro-Industrial Residues

by
Danai Ioanna Koukoumaki
1,
Seraphim Papanikolaou
2,*,
Christina Rogka
2 and
Dimitris Sarris
1,*
1
Laboratory of Physico-Chemical and Biotechnological Valorization of Food By-Products, Department of Food Science & Nutrition, School of the Environment, University of the Aegean, Leoforos Dimokratias 66, Lemnos, 81400 Myrina, Greece
2
Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece
*
Authors to whom correspondence should be addressed.
Sustainability 2025, 17(22), 10082; https://doi.org/10.3390/su172210082
Submission received: 24 March 2025 / Revised: 22 August 2025 / Accepted: 7 November 2025 / Published: 11 November 2025
(This article belongs to the Special Issue Innovations in Agri-Food Biotechnology for Sustainable and Secure Food Production)
Versions Notes

Abstract

Within the framework of sustainability, the parallel valorization of two challenging industrial (crude glycerol) and agricultural (olive mill wastewaters—OMWs) residues by the yeast Yarrowia lipolytica was examined. The rationale of this study was to evaluate the potential of the Y. lipolytica strain ACA-YC 5031 to produce valuable metabolites under a wide range of pH values and increasing NaCl concentrations in agro-industrial blends. OMWs were used as both microbial substrate and process water, and despite high levels of phenolic compounds in the medium and the simultaneously high initial concentrations of NaCl, appreciable quantities of dry cell weight (DCW) and metabolites were synthesized. Moreover, the growth of the strain under non-aseptic conditions was examined. The simultaneous effect of low pH (3.0) and the presence of OMWs (~2.0 g/L) notably increased the extracellular production of erythritol and the accumulation of cellular lipids (reaching Erymax = 18.3 g/L and DCW = 38.6% w/w, respectively). In media with low pH (3.0) and high NaCl concentration (5.0% w/v), a metabolic shift towards erythritol secretion was observed (Erymax = 27.2 g/L, with YEry/Glol = 0.46 g/g). Oleic acid accumulation was enhanced by OMW presence in the medium.
Keywords: OMWs; Single-Cell Oil; polyols; Yarrowia lipolytica; non-aseptic conditions; sustainability OMWs; Single-Cell Oil; polyols; Yarrowia lipolytica; non-aseptic conditions; sustainability

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MDPI and ACS Style

Koukoumaki, D.I.; Papanikolaou, S.; Rogka, C.; Sarris, D. Sustainable Production of Added-Value Metabolic Compounds Under Adverse Culture Conditions by Microorganisms: A Case Study of Yarrowia lipolytica Strain Cultivated on Agro-Industrial Residues. Sustainability 2025, 17, 10082. https://doi.org/10.3390/su172210082

AMA Style

Koukoumaki DI, Papanikolaou S, Rogka C, Sarris D. Sustainable Production of Added-Value Metabolic Compounds Under Adverse Culture Conditions by Microorganisms: A Case Study of Yarrowia lipolytica Strain Cultivated on Agro-Industrial Residues. Sustainability. 2025; 17(22):10082. https://doi.org/10.3390/su172210082

Chicago/Turabian Style

Koukoumaki, Danai Ioanna, Seraphim Papanikolaou, Christina Rogka, and Dimitris Sarris. 2025. "Sustainable Production of Added-Value Metabolic Compounds Under Adverse Culture Conditions by Microorganisms: A Case Study of Yarrowia lipolytica Strain Cultivated on Agro-Industrial Residues" Sustainability 17, no. 22: 10082. https://doi.org/10.3390/su172210082

APA Style

Koukoumaki, D. I., Papanikolaou, S., Rogka, C., & Sarris, D. (2025). Sustainable Production of Added-Value Metabolic Compounds Under Adverse Culture Conditions by Microorganisms: A Case Study of Yarrowia lipolytica Strain Cultivated on Agro-Industrial Residues. Sustainability, 17(22), 10082. https://doi.org/10.3390/su172210082

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