Investigating Wood Decaying Fungi Diversity in Central Siberia, Russia Using ITS Sequence Analysis and Interaction with Host Trees
, Min-Ji Kim 4, Myung Soo Park 1, Seung-Yoon Oh 5, Ki Hyeong Park 1, Jonathan J. Fong 6 and Young Woon Lim 1,*Round 1
Reviewer 1 Report
In this paper, authors reported Investigating wood decaying fungi diversity in Central Siberia, Russia using ITS sequence analysis and interaction with host trees. The authors, after a favorable and thorough review of the literature, performed high-level research of wood-decay fungi with great care.
The content of this publication is very interesting and important.
Line 110: I suggest that you add temperature-time profile of the PCR reaction, except for reference only.
Table 1: Thanks to the description of the results in the subsection Host preference, I think that Table 1. should be moved to the Supplementary Materials.
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
MS #Sustainability-744193
This paper is reporting occurrence of mushrooms of white-rot and brown-rot fungi based on field work in Central Siberia, Russia. The fungi were identified by morphological observation together with similarity search of nucleotide sequence of ITS region. Also, specimens have been deposited to public collection.
This report would provide merits especially for readers in the field of fungal ecology. To maximize the merits I should raise several concerns/questions as described below, requiring of the authors’ careful consideration.
- L82-92
More detailed description about sampling is requested for subsections below.
1-1. ABS/CBD (Nagoya Protocol)
Do the authors comply with regulations of Nagoya Protocol? The specimens originated from Russia have been deposited to a collection in South Korea. I am worrying that the authors might face some serious problem when the article will be publicized.
As I do not know well about the domestic regulations in South Korea and Russia about ABS/CBD, this would be a nonsense comment.
1-2. Effort of sampling
The extent of sampling effort would be very informative for readers.
How many days (hours) did the authors make effort to find WDF for each sampling site? How large area did the authors (how many persons) survey for each sampling site?
1-3. Information on the sampling sites
Please specify GPS coordinate of the sampling sites. Also, please add more information about the environment (e.g. vegetation, annual average temperature, max-min temperature, Köppen climate classification).
1-4. Selection of sampling site
Why did the authors select the locations as sampling site? If there was any specific reason, please describe it.
1-5. Target plant species
Why did the authors select the six plant species? Are they dominant there, or common among the seven sampling sites?
Description of tree species here would be enriched as shown below.
Pinus sylvestris (5 samples; Gymnosperm, Pinaceae)
- L60
Plains, [13-16] -> Plains [13-16]
- L106
Please specify the date of access to Index Fungorum.
Also, delete a space before ‘Names.asp’.
- L108
Did the authors extract DNA from internal tissue of mushroom?
Please describe the method briefly.
- L129
98% similarity the -> 98% similarity to the
- L245-246
The description is for ‘Figure S1’. However, title of the supplementary material is ‘Supplementary table 1’. Please make sure the consistency.
- L250
YY Lim -> YW Lim
(Correct?)
Author Response
Please see the attachment
Author Response File: Author Response.pdf
