Croton argyrophyllus Kunth Essential Oil-Loaded Solid Lipid Nanoparticles: Evaluation of Release Profile, Antioxidant Activity and Cytotoxicity in a Neuroblastoma Cell Line
Abstract
:1. Introduction
2. Material and Methods
2.1. Materials
2.2. Production of Solid Lipid Nanoparticles
2.3. Mean Particle Size and Polydispersity Index
2.4. Encapsulation Efficiency (EE) and Loading Capacity (LC)
2.5. Differential Scanning Calorimetry
2.6. In Vitro Release of CAEO-SLN
2.7. In Vitro Lipid Peroxidation Assay
2.8. In Vitro Antioxidant Activity against Free Radical DPPH
2.9. Cell Culture and Cytotoxicity Assay
2.10. Statistical Analysis
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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µg/mL | % Scavenging ± SD |
---|---|
1 | 1.76 ± 0.32 |
2 | 5.92 ± 0.18 |
3 | 7.83 ± 1.21 |
4 | 11.14 ± 1.07 |
5 | 17.23 ± 0.61 |
10 | 22.30 ± 1.42 |
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Souto, E.B.; Severino, P.; Marques, C.; Andrade, L.N.; Durazzo, A.; Lucarini, M.; Atanasov, A.G.; El Maimouni, S.; Novellino, E.; Santini, A. Croton argyrophyllus Kunth Essential Oil-Loaded Solid Lipid Nanoparticles: Evaluation of Release Profile, Antioxidant Activity and Cytotoxicity in a Neuroblastoma Cell Line. Sustainability 2020, 12, 7697. https://doi.org/10.3390/su12187697
Souto EB, Severino P, Marques C, Andrade LN, Durazzo A, Lucarini M, Atanasov AG, El Maimouni S, Novellino E, Santini A. Croton argyrophyllus Kunth Essential Oil-Loaded Solid Lipid Nanoparticles: Evaluation of Release Profile, Antioxidant Activity and Cytotoxicity in a Neuroblastoma Cell Line. Sustainability. 2020; 12(18):7697. https://doi.org/10.3390/su12187697
Chicago/Turabian StyleSouto, Eliana B., Patricia Severino, Conrado Marques, Luciana N. Andrade, Alessandra Durazzo, Massimo Lucarini, Atanas G. Atanasov, Soukaina El Maimouni, Ettore Novellino, and Antonello Santini. 2020. "Croton argyrophyllus Kunth Essential Oil-Loaded Solid Lipid Nanoparticles: Evaluation of Release Profile, Antioxidant Activity and Cytotoxicity in a Neuroblastoma Cell Line" Sustainability 12, no. 18: 7697. https://doi.org/10.3390/su12187697