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Microbiology Research
Volume 14
Issue 4
10.3390/microbiolres14040132
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Communication

Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome

by
Shulin Fu
1,2,†,
Xinyue Tian
1,2,†,
Jingyang Li
1,2,†,
Yuzhen Yuan
1,2,
Jing He
1,2,
Chun Peng
1,2,
Ling Guo
1,2,
Chun Ye
1,2,
Yu Liu
1,2,
Bingbing Zong
1,2 and
Yinsheng Qiu
1,2,*
1
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China
2
Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan 430023, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microbiol. Res. 2023, 14(4), 1956-1968; https://doi.org/10.3390/microbiolres14040132
Submission received: 25 August 2023 / Revised: 3 October 2023 / Accepted: 3 November 2023 / Published: 20 November 2023
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Abstract

The gut microbiota is engaged in multiple interactions affecting host health, and gut dysbiosis can lead to many diseases. However, the effects of acetylcysteine (NAC) on the gut microbiome composition in pigs using metagenomic sequencing have not been reported. In this study, we used metagenome sequencing to study the effects of NAC on the pig gut microbiome. Sequencing results showed that microbial diversity was changed after NAC treatment. Antibiotic Resistance Genes Database (ARDB) analysis demonstrated that the main genes modified were macb, tsnr, norm, bl2be-per, vansb and pbp1b in the NAC group. Our data showed that NAC could affect microbial distribution at the phylum, gene and species levels. At the species level, NAC significantly increased the abundances of Megasphaera, Lactobacillus reuteri and Megasphaeraelsdenii and reduced the abundances of Phascolarctobacterium succinatutens, Prevotellacopri and Selenomonasbovis compared with the control group. In addition, Gene Ontology (GO) analysis revealed that in the NAC group, cellular process, metabolic process and single-organism process were the dominant terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that RNA transport, MAPK signaling pathway, cell cycle, glycosylphosphatidylinositol (GPI)-anchor biosynthesis and VEGF signaling pathway were the dominant signaling pathways in the NAC group. In conclusion, our results suggest that NAC may modify the piglet gut microbiome composition and these findings might provide a new strategy for maintaining animal and human health in the future.
Keywords: acetylcysteine; gut microbiome; pig; metagenomic sequencing; modification acetylcysteine; gut microbiome; pig; metagenomic sequencing; modification

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MDPI and ACS Style

Fu, S.; Tian, X.; Li, J.; Yuan, Y.; He, J.; Peng, C.; Guo, L.; Ye, C.; Liu, Y.; Zong, B.; et al. Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome. Microbiol. Res. 2023, 14, 1956-1968. https://doi.org/10.3390/microbiolres14040132

AMA Style

Fu S, Tian X, Li J, Yuan Y, He J, Peng C, Guo L, Ye C, Liu Y, Zong B, et al. Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome. Microbiology Research. 2023; 14(4):1956-1968. https://doi.org/10.3390/microbiolres14040132

Chicago/Turabian Style

Fu, Shulin, Xinyue Tian, Jingyang Li, Yuzhen Yuan, Jing He, Chun Peng, Ling Guo, Chun Ye, Yu Liu, Bingbing Zong, and et al. 2023. "Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome" Microbiology Research 14, no. 4: 1956-1968. https://doi.org/10.3390/microbiolres14040132

APA Style

Fu, S., Tian, X., Li, J., Yuan, Y., He, J., Peng, C., Guo, L., Ye, C., Liu, Y., Zong, B., & Qiu, Y. (2023). Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome. Microbiology Research, 14(4), 1956-1968. https://doi.org/10.3390/microbiolres14040132

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