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Microbiology Research
Volume 13
Issue 4
10.3390/microbiolres13040061
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Article

Screening for Escherichia coli in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal

by
Sagar Regmi
1,*,
Prem Lal Mahato
2,
Sachin Upadhayaya
1,
Hari Marasini
1,
Raju Prasad Neupane
1,
Janashrit Shrestha
1,
Rebanta Kumar Bhattarai
3 and
Santosh Dhakal
4,*
1
Faculty of Animal Science, Veterinary Science and Fisheries, Agriculture and Forestry University, Rampur, Chitwan 44200, Nepal
2
College of Tropical Agriculture and Human Resources, University of Hawai’i at Manoa, Honolulu, HI 96848, USA
3
Department of Microbiology and Parasitology, Agriculture and Forestry University, Rampur, Chitwan 44200, Nepal
4
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
*
Authors to whom correspondence should be addressed.
Microbiol. Res. 2022, 13(4), 872-881; https://doi.org/10.3390/microbiolres13040061
Submission received: 23 September 2022 / Revised: 17 October 2022 / Accepted: 25 October 2022 / Published: 28 October 2022
(This article belongs to the Collection Public Health and Quality Aspects Related to Animal Productions)
Versions Notes

Abstract

In this study, chopping board meat samples collected from meat shops of Bharatpur Metropolitan City, Nepal, were screened for the presence of Escherichia coli (E. coli), with a special emphasis on the identification of extended-spectrum β-lactamase (ESBL)-producing strains. Representatives from the meat shops were also interviewed to understand the sanitary status and hygienic practices. E. coli bacteria were detected in one third (33/99) of the meat samples, while none of the samples had ESBL-producing strains. While 60.6% (60/99) of the meat shop personnel wore protective clothing, 15.15% (15/99) used gloves, and only 5.05% (5/99) had separate equipment for cleaning the viscera of animals. This study highlights the need for the regular screening of meat samples to identify pathogenic bacteria such as E. coli and for improvements in the sanitary status and hygienic practices of retail meat shops in Bharatpur Metropolitan City, Nepal.
Keywords: antimicrobial resistance; Escherichia coli; extended-spectrum β-lactamase; slaughterhouse hygiene antimicrobial resistance; Escherichia coli; extended-spectrum β-lactamase; slaughterhouse hygiene

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MDPI and ACS Style

Regmi, S.; Mahato, P.L.; Upadhayaya, S.; Marasini, H.; Neupane, R.P.; Shrestha, J.; Bhattarai, R.K.; Dhakal, S. Screening for Escherichia coli in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal. Microbiol. Res. 2022, 13, 872-881. https://doi.org/10.3390/microbiolres13040061

AMA Style

Regmi S, Mahato PL, Upadhayaya S, Marasini H, Neupane RP, Shrestha J, Bhattarai RK, Dhakal S. Screening for Escherichia coli in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal. Microbiology Research. 2022; 13(4):872-881. https://doi.org/10.3390/microbiolres13040061

Chicago/Turabian Style

Regmi, Sagar, Prem Lal Mahato, Sachin Upadhayaya, Hari Marasini, Raju Prasad Neupane, Janashrit Shrestha, Rebanta Kumar Bhattarai, and Santosh Dhakal. 2022. "Screening for Escherichia coli in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal" Microbiology Research 13, no. 4: 872-881. https://doi.org/10.3390/microbiolres13040061

APA Style

Regmi, S., Mahato, P. L., Upadhayaya, S., Marasini, H., Neupane, R. P., Shrestha, J., Bhattarai, R. K., & Dhakal, S. (2022). Screening for Escherichia coli in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal. Microbiology Research, 13(4), 872-881. https://doi.org/10.3390/microbiolres13040061

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