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Pharmaceutics
Volume 16
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10.3390/pharmaceutics16080988
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Article

Nanosized Complexes of the Proteolytic Enzyme Serratiopeptidase with Cationic Block Copolymer Micelles Enhance the Proliferation and Migration of Human Cells

by
Katya Kamenova
1,
Anna Prancheva
1,
Lyubomira Radeva
2,
Krassimira Yoncheva
2,
Maya M. Zaharieva
3,
Hristo M. Najdenski
3 and
Petar D. Petrov
1,*
1
Institute of Polymers, Bulgarian Academy of Sciences, bl.103 Akad. G. Bonchev Str., 1113 Sofia, Bulgaria
2
Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria
3
The Stephan Angeloff Institute of Microbiology, 1113 Sofia, Bulgaria
*
Author to whom correspondence should be addressed.
Pharmaceutics 2024, 16(8), 988; https://doi.org/10.3390/pharmaceutics16080988
Submission received: 3 July 2024 / Revised: 17 July 2024 / Accepted: 24 July 2024 / Published: 25 July 2024
(This article belongs to the Special Issue Self-Assembled Amphiphilic Copolymers in Drug Delivery, 2nd Edition)
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Abstract

In this study, we describe the preparation of the cationic block copolymer nanocarriers of the proteolytic enzyme serratiopeptidase (SER). Firstly, an amphiphilic poly(2-(dimethylamino)ethyl methacrylate)-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA9-b-PCL35-b-PDMAEMA9) triblock copolymer was synthesized by reversible addition-fragmentation chain-transfer (RAFT) polymerization. Then, cationic micellar nanocarriers consisting of a PCL hydrophobic core and a PDMAEMA hydrophilic shell were formed by the solvent evaporation method. SER was loaded into the polymeric micelles by electrostatic interaction between the positively charged micellar shell and the negatively charged enzyme molecules. The particle size, zeta potential, and colloid stability of complexes as a function of SER concentration were investigated by dynamic and electrophoretic light scattering. It was found that SER retained its proteolytic activity after immobilization in polymeric carriers. Moreover, the complexes have a concentration-dependent enhancing effect on the proliferation and migration of human keratinocyte HaCaT and gingival fibroblast HGF cells.
Keywords: nanocarriers; polymeric micelles; serratiopeptidase; RAFT polymerization; cell proliferation; cell migration; wound healing nanocarriers; polymeric micelles; serratiopeptidase; RAFT polymerization; cell proliferation; cell migration; wound healing

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MDPI and ACS Style

Kamenova, K.; Prancheva, A.; Radeva, L.; Yoncheva, K.; Zaharieva, M.M.; Najdenski, H.M.; Petrov, P.D. Nanosized Complexes of the Proteolytic Enzyme Serratiopeptidase with Cationic Block Copolymer Micelles Enhance the Proliferation and Migration of Human Cells. Pharmaceutics 2024, 16, 988. https://doi.org/10.3390/pharmaceutics16080988

AMA Style

Kamenova K, Prancheva A, Radeva L, Yoncheva K, Zaharieva MM, Najdenski HM, Petrov PD. Nanosized Complexes of the Proteolytic Enzyme Serratiopeptidase with Cationic Block Copolymer Micelles Enhance the Proliferation and Migration of Human Cells. Pharmaceutics. 2024; 16(8):988. https://doi.org/10.3390/pharmaceutics16080988

Chicago/Turabian Style

Kamenova, Katya, Anna Prancheva, Lyubomira Radeva, Krassimira Yoncheva, Maya M. Zaharieva, Hristo M. Najdenski, and Petar D. Petrov. 2024. "Nanosized Complexes of the Proteolytic Enzyme Serratiopeptidase with Cationic Block Copolymer Micelles Enhance the Proliferation and Migration of Human Cells" Pharmaceutics 16, no. 8: 988. https://doi.org/10.3390/pharmaceutics16080988

APA Style

Kamenova, K., Prancheva, A., Radeva, L., Yoncheva, K., Zaharieva, M. M., Najdenski, H. M., & Petrov, P. D. (2024). Nanosized Complexes of the Proteolytic Enzyme Serratiopeptidase with Cationic Block Copolymer Micelles Enhance the Proliferation and Migration of Human Cells. Pharmaceutics, 16(8), 988. https://doi.org/10.3390/pharmaceutics16080988

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