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Pharmaceutics
Volume 16
Issue 7
10.3390/pharmaceutics16070860
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Article

Antimicrobial Peptide Screening for Designing Custom Bactericidal Hydrogels

by
Matthias Recktenwald
1,
Muskanjot Kaur
2,
Mohammed M. Benmassaoud
1,
Aryanna Copling
3,
Tulika Khanna
4,
Michael Curry
2,
Dennise Cortes
2,
Gilbert Fleischer
2,
Valerie J. Carabetta
2,* and
Sebastián L. Vega
1,5,*
1
Department of Biomedical Engineering, Rowan University, Glassboro, NJ 08028, USA
2
Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ 08103, USA
3
Department of Translational Biomedical Sciences, Rowan University, Glassboro, NJ 08028, USA
4
Department of Biological Sciences, Rowan University, Glassboro, NJ 08028, USA
5
Department of Orthopedic Surgery, Cooper Medical School of Rowan University, Camden, NJ 08103, USA
*
Authors to whom correspondence should be addressed.
Pharmaceutics 2024, 16(7), 860; https://doi.org/10.3390/pharmaceutics16070860
Submission received: 3 June 2024 / Revised: 21 June 2024 / Accepted: 25 June 2024 / Published: 27 June 2024
(This article belongs to the Special Issue Recent Advances in the Prevention and Eradication Strategies for Combating Biofilm-Related Infections)
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Abstract

Staphylococcus aureus (S. aureus) is an opportunistic pathogen that lives on surfaces and skin and can cause serious infections inside the body. Antimicrobial peptides (AMPs) are part of the innate immune system and can eliminate pathogens, including bacteria and viruses, and are a promising alternative to antibiotics. Although studies have reported that AMP-functionalized hydrogels can prevent bacterial adhesion and biofilm formation, AMP dosing and the combined effects of multiple AMPs are not well understood. Here, three AMPs with different antibacterial properties were synthesized and the soluble minimum inhibitory concentrations (MICs) of each AMP against methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) were determined. Hydrogels with immobilized AMPs at their MIC (DD13-RIP 27.5 µM; indolicidin 43.8 µM; P10 120 µM) were effective in preventing MRSA adhesion and biofilm formation. Checkerboard AMP screens identified synergy between indolicidin (3.1 µM) and P10 (12.5 µM) based on soluble fractional inhibitory concentration indices (FICIs) against MRSA, and hydrogels formed with these AMPs at half of their synergistic concentrations (total peptide concentration, 7.8 µM) were highly efficacious in killing MRSA. Mammalian cells cultured atop these hydrogels were highly viable, demonstrating that these AMP hydrogels are biocompatible and selectively eradicate bacteria, based on soluble checkerboard-screening data.
Keywords: antimicrobial peptides; AMP screening; hydrogels; thiol-norbornene; medical device infections antimicrobial peptides; AMP screening; hydrogels; thiol-norbornene; medical device infections

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MDPI and ACS Style

Recktenwald, M.; Kaur, M.; Benmassaoud, M.M.; Copling, A.; Khanna, T.; Curry, M.; Cortes, D.; Fleischer, G.; Carabetta, V.J.; Vega, S.L. Antimicrobial Peptide Screening for Designing Custom Bactericidal Hydrogels. Pharmaceutics 2024, 16, 860. https://doi.org/10.3390/pharmaceutics16070860

AMA Style

Recktenwald M, Kaur M, Benmassaoud MM, Copling A, Khanna T, Curry M, Cortes D, Fleischer G, Carabetta VJ, Vega SL. Antimicrobial Peptide Screening for Designing Custom Bactericidal Hydrogels. Pharmaceutics. 2024; 16(7):860. https://doi.org/10.3390/pharmaceutics16070860

Chicago/Turabian Style

Recktenwald, Matthias, Muskanjot Kaur, Mohammed M. Benmassaoud, Aryanna Copling, Tulika Khanna, Michael Curry, Dennise Cortes, Gilbert Fleischer, Valerie J. Carabetta, and Sebastián L. Vega. 2024. "Antimicrobial Peptide Screening for Designing Custom Bactericidal Hydrogels" Pharmaceutics 16, no. 7: 860. https://doi.org/10.3390/pharmaceutics16070860

APA Style

Recktenwald, M., Kaur, M., Benmassaoud, M. M., Copling, A., Khanna, T., Curry, M., Cortes, D., Fleischer, G., Carabetta, V. J., & Vega, S. L. (2024). Antimicrobial Peptide Screening for Designing Custom Bactericidal Hydrogels. Pharmaceutics, 16(7), 860. https://doi.org/10.3390/pharmaceutics16070860

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