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Pharmaceutics
Volume 14
Issue 9
10.3390/pharmaceutics14091934
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Article

Development and Evaluation of a Thermosensitive In Situ Gel Formulation for Intravaginal Delivery of Lactobacillus gasseri

by
Ye Zhao
1,*,†,
Tianyuan Wang
1,†,
Ziyi Chen
1,
Hao Ren
1,
Ping Song
2,
Yingying Zhu
1,
Shan Liang
1 and
Chimeng Tzeng
3,4,*
1
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, China
2
School of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, China
3
Translational Medicine Research Center-Key Laboratory for Cancer T-Cell Theragnostic and Clinical Translation, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361005, China
4
Xiamen Chang Gung Hospital Medical Research Center, Xiamen 361022, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to the paper.
Pharmaceutics 2022, 14(9), 1934; https://doi.org/10.3390/pharmaceutics14091934
Submission received: 27 July 2022 / Revised: 20 August 2022 / Accepted: 8 September 2022 / Published: 13 September 2022
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Abstract

In situ administration of vaginal probiotics has been proposed as an effective prevention strategy against gynecological diseases caused by microecological disorders. In this study, a thermosensitive in situ gel formulation was prepared for intravaginal delivery of Lactobacillus gasseri(L. gasseri). The optimized formulation was characterized for the rheological properties, in vitro release properties, and microencapsulation efficiency. The mixtures of poloxamer 407 (26.0% w/w) and 188 (9.0% w/w) produced an increase in gelation extent at 37 °C after dilution in simulated vaginal fluid (SVF). The presence of a low concentration of hyaluronic acid (HA, 0.3% w/w) improved the mucoadhesive properties and the capability to gel at 37 °C. Additionally, the viability of L. gasseri encapsulated with alginate or via co-extrusion technique with fructooligosaccharide (FOS, 0.5% w/w) was maintained at 11 log CFU/mL for eight weeks at 4 °C. In conclusion, the evaluation of the in situ thermosensitive gel formulation was shown to be efficacious for intravaginal delivery of L. gasseri with suitable textural and rheological properties.
Keywords: Lactobacillus gasseri; thermosensitive; vaginal gel; poloxamer; fructooligosaccharide; hyaluronic acid Lactobacillus gasseri; thermosensitive; vaginal gel; poloxamer; fructooligosaccharide; hyaluronic acid
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MDPI and ACS Style

Zhao, Y.; Wang, T.; Chen, Z.; Ren, H.; Song, P.; Zhu, Y.; Liang, S.; Tzeng, C. Development and Evaluation of a Thermosensitive In Situ Gel Formulation for Intravaginal Delivery of Lactobacillus gasseri. Pharmaceutics 2022, 14, 1934. https://doi.org/10.3390/pharmaceutics14091934

AMA Style

Zhao Y, Wang T, Chen Z, Ren H, Song P, Zhu Y, Liang S, Tzeng C. Development and Evaluation of a Thermosensitive In Situ Gel Formulation for Intravaginal Delivery of Lactobacillus gasseri. Pharmaceutics. 2022; 14(9):1934. https://doi.org/10.3390/pharmaceutics14091934

Chicago/Turabian Style

Zhao, Ye, Tianyuan Wang, Ziyi Chen, Hao Ren, Ping Song, Yingying Zhu, Shan Liang, and Chimeng Tzeng. 2022. "Development and Evaluation of a Thermosensitive In Situ Gel Formulation for Intravaginal Delivery of Lactobacillus gasseri" Pharmaceutics 14, no. 9: 1934. https://doi.org/10.3390/pharmaceutics14091934

APA Style

Zhao, Y., Wang, T., Chen, Z., Ren, H., Song, P., Zhu, Y., Liang, S., & Tzeng, C. (2022). Development and Evaluation of a Thermosensitive In Situ Gel Formulation for Intravaginal Delivery of Lactobacillus gasseri. Pharmaceutics, 14(9), 1934. https://doi.org/10.3390/pharmaceutics14091934

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