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Article

Quantification of Polyethylene Glycol 400 Excreted in the Urine by MALDI-TOF Mass Spectrometry

1
Department of Applied Chemistry, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary
2
Doctoral School of Chemistry, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary
3
Today’s Life Science and Research Kft., Bulcsú utca 20/A, H-2120 Dunakeszi, Hungary
*
Author to whom correspondence should be addressed.
Academic Editor: Sangkyu Lee
Pharmaceutics 2022, 14(7), 1341; https://doi.org/10.3390/pharmaceutics14071341
Received: 8 April 2022 / Revised: 27 April 2022 / Accepted: 29 April 2022 / Published: 24 June 2022
Polyethylene glycol 400 (PEG 400) was used as a permeability probe to examine the gastrointestinal tract which can be involved in the pathogenesis of some inflammatory and autoimmune diseases. A novel methodology was developed and validated for the quantitation of PEG 400 excreted in human urine after oral administration using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The excretion ratios were determined for the most intense ions corresponding to nine PEG 400 oligomers. The relative error of accuracy was between –6.0% and 8.5%, and the relative standard deviation (RSD) of the precision was below 15%. Our method was successfully applied in a large-scale experimental study involving nearly two hundred volunteers. Due to the large number of measurements, detailed and reliable statistical analysis was performed. No significant difference was found between the male and female group of volunteers at 0.05 significance level, except the two largest PEG oligomers. However, the average excretion ratios of the male volunteers are greater than that of the women for all the nine PEG oligomers, suggesting a difference in the intestinal permeability between men and women. View Full-Text
Keywords: polyethylene glycol; intestinal permeability; MALDI-TOF; quantification polyethylene glycol; intestinal permeability; MALDI-TOF; quantification
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MDPI and ACS Style

Kuki, Á.; Hashimov, M.; Nagy, T.; Tóth, C.; Zsuga, M.; Kéki, S. Quantification of Polyethylene Glycol 400 Excreted in the Urine by MALDI-TOF Mass Spectrometry. Pharmaceutics 2022, 14, 1341. https://doi.org/10.3390/pharmaceutics14071341

AMA Style

Kuki Á, Hashimov M, Nagy T, Tóth C, Zsuga M, Kéki S. Quantification of Polyethylene Glycol 400 Excreted in the Urine by MALDI-TOF Mass Spectrometry. Pharmaceutics. 2022; 14(7):1341. https://doi.org/10.3390/pharmaceutics14071341

Chicago/Turabian Style

Kuki, Ákos, Mahir Hashimov, Tibor Nagy, Csaba Tóth, Miklos Zsuga, and Sándor Kéki. 2022. "Quantification of Polyethylene Glycol 400 Excreted in the Urine by MALDI-TOF Mass Spectrometry" Pharmaceutics 14, no. 7: 1341. https://doi.org/10.3390/pharmaceutics14071341

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