Semi-Solid Pharmaceutical Formulations for the Delivery of Papain Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Nanoparticles Synthesis
2.2.2. Development of Semi-Solid Formulations
2.2.3. Papain Loading
2.2.4. Characterization
Nanoparticles Size
Nanoparticles Crosslinking
Stability Assessment
Scanning Electron Microscopy
Protein Distribution
Proteolytic Activity in the Gel Formulation
Papain Release
Cytotoxicity
3. Results and Discussion
3.1. Nanoparticles
3.2. Semi-Solid Formulation Stability Assessment
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | Size (d.nm) | Polydispersity Index | Enzymatic Activity |
---|---|---|---|
Nanopapain | 7.0 ± 0.1 | 0.34 | 88.5% ± 7.39 |
Native papain | 3.3 ± 0.4 | 0.39 | 100% ± 1.03 |
Formulation | Content | Centrifugation | Aspect | Color | Smell |
---|---|---|---|---|---|
Gel A | 0.2% PVA 2% CMC | stable | Homogeneous and translucent | Colorless | No change |
Gel A + papain (control) | Gel A + 0.2% native papain | stable | Homogeneous and translucent | Colorless | No change |
Gel A + nanopapain | Gel A + 0.2% nanopapain | stable | Homogeneous and translucent | Colorless | No change |
Gel B | 0.2% PVA 3% CMC | stable | Homogeneous and translucent | Colorless | No change |
Gel B + papain (control) | Gel B + 0.2% native papain | stable | Homogeneous and translucent | Colorless | No change |
Gel B + nanopapain | Gel B + 0.2% nanopapain | stable | Homogeneous and translucent | Colorless | No change |
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Lima, C.S.A.d.; Varca, J.P.R.O.; Nogueira, K.M.; Fazolin, G.N.; Freitas, L.F.d.; Souza, E.W.d.; Lugão, A.B.; Varca, G.H.C. Semi-Solid Pharmaceutical Formulations for the Delivery of Papain Nanoparticles. Pharmaceutics 2020, 12, 1170. https://doi.org/10.3390/pharmaceutics12121170
Lima CSAd, Varca JPRO, Nogueira KM, Fazolin GN, Freitas LFd, Souza EWd, Lugão AB, Varca GHC. Semi-Solid Pharmaceutical Formulations for the Delivery of Papain Nanoparticles. Pharmaceutics. 2020; 12(12):1170. https://doi.org/10.3390/pharmaceutics12121170
Chicago/Turabian StyleLima, Caroline S. A. de, Justine P. R. O. Varca, Kamila M. Nogueira, Gabriela N. Fazolin, Lucas F. de Freitas, Eliseu W. de Souza, Ademar B. Lugão, and Gustavo. H. C. Varca. 2020. "Semi-Solid Pharmaceutical Formulations for the Delivery of Papain Nanoparticles" Pharmaceutics 12, no. 12: 1170. https://doi.org/10.3390/pharmaceutics12121170