Comprehensive Analysis of the Safety Profile of a Single-Stranded RNA Nano-Structure Adjuvant
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Doi: 10.5281/zenodo.3354374
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Description: Figure S1: Brief structure of CrPV IGR IRES-derived ssRNA nano-structure adjuvant. Figure S2: Dose-dependent cell viabilities of various cell lines treated with the ssRNA nano-structure adjuvant, using MTT assays. Relative viabilities of (A) A549 cells and (B) HepG2 cells were compared to negative control (0 concentration of ssRNA nano-structure adjuvant) from 24 h to 72 h, based on the ssRNA concentration. Figure S3: Pro-inflammatory cytokine induction in RAW 264.7 cells after treatment with poly I:C and ssRNA nano-structure adjuvant. Supplementary method: Real-time PCR for proinflammatory cytokine in RAW 264.7 cells after treatment with poly I:C and ssRNA nano-structure adjuvant. Figure S4: Serum levels of MCP-1, TNF-α, IL-1β, IL-6, and IL12p70. Sera from mice in groups 1–5 were collected 1 day after the last immunization, and sera from mice in group 6 were collected 2 weeks after the last immunization.
Park, H.-J.; Ko, H.L.; Won, D.-H.; Hwang, D.-B.; Shin, Y.-S.; Kwak, H.-W.; Kim, H.-J.; Yun, J.-W.; Nam, J.-H. Comprehensive Analysis of the Safety Profile of a Single-Stranded RNA Nano-Structure Adjuvant. Pharmaceutics 2019, 11, 464. https://doi.org/10.3390/pharmaceutics11090464
Park H-J, Ko HL, Won D-H, Hwang D-B, Shin Y-S, Kwak H-W, Kim H-J, Yun J-W, Nam J-H. Comprehensive Analysis of the Safety Profile of a Single-Stranded RNA Nano-Structure Adjuvant. Pharmaceutics. 2019; 11(9):464. https://doi.org/10.3390/pharmaceutics11090464
Chicago/Turabian StylePark, Hyeong-Jun, Hae L. Ko, Dong-Hoon Won, Da-Bin Hwang, Yoo-Sub Shin, Hye-Won Kwak, Hye-Jung Kim, Jun-Won Yun, and Jae-Hwan Nam. 2019. "Comprehensive Analysis of the Safety Profile of a Single-Stranded RNA Nano-Structure Adjuvant" Pharmaceutics 11, no. 9: 464. https://doi.org/10.3390/pharmaceutics11090464