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Open AccessArticle

Sustained Release of Minor-Groove-Binding Antibiotic Netropsin from Calcium-Coated Groove-Rich DNA Particles

Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemungu, Seoul 02504, Korea
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Author to whom correspondence should be addressed.
Pharmaceutics 2019, 11(8), 387; https://doi.org/10.3390/pharmaceutics11080387
Received: 20 May 2019 / Revised: 14 July 2019 / Accepted: 22 July 2019 / Published: 2 August 2019
(This article belongs to the Special Issue Bioinspired Design in Drug Delivery)
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Abstract

Control of the release properties of drugs has been considered a key factor in the development of drug delivery systems (DDSs). However, drug delivery has limitations including cytotoxicity, low loading efficiency, and burst release. To overcome these challenges, nano or micro-particles have been suggested as carrier systems to deliver chemical drugs. Herein, nano-sized DNA particles (DNAp) were manufactured to deliver netropsin, which is known to bind to DNA minor grooves. The rationally designed particles with exposed rich minor grooves were prepared by DNAp synthesis via rolling circle amplification (RCA). DNAp could load large quantities of netropsin in its minor grooves. An analytical method was also developed for the quantification of netropsin binding to DNAp by UV–visible spectrometry. Moreover, controlled release of netropsin was achieved by forming a layer of Ca2+ on the DNAp (CaDNAp). As a proof of concept, the sustained release of netropsin by CaDNAp highlights the potential of the DNAp-based delivery approach. View Full-Text
Keywords: DNA particle (DNAp); netropsin; minor groove binder; calcium; rolling circle amplification (RCA) DNA particle (DNAp); netropsin; minor groove binder; calcium; rolling circle amplification (RCA)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jeon, H.; Nam, H.; Lee, J.B. Sustained Release of Minor-Groove-Binding Antibiotic Netropsin from Calcium-Coated Groove-Rich DNA Particles. Pharmaceutics 2019, 11, 387.

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