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In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing

1
Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 211007, India
2
Nanotechnology Application Centre, Faculty of Science, University of Allahabad, Allahabad 211002, India
3
Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad 211007, India
4
E-Spin Nanotech Pvt Ltd., Kanpur 208016, India
5
Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, Germany
6
Physical Intelligence Department, Max Planck Institute for Intelligent Systems, 70569 Stuttgart, Germany
*
Authors to whom correspondence should be addressed.
Pharmaceutics 2019, 11(4), 180; https://doi.org/10.3390/pharmaceutics11040180
Received: 13 March 2019 / Revised: 2 April 2019 / Accepted: 11 April 2019 / Published: 14 April 2019
(This article belongs to the Special Issue Recent Development of Electrospinning for Drug Delivery)
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Abstract

Tissue engineering technologies involving growth factors have produced one of the most advanced generations of diabetic wound healing solutions. Using this approach, a nanocomposite carrier was designed using Poly(d,l-lactide-co-glycolide) (PLGA)/Gelatin polymer solutions for the simultaneous release of recombinant human epidermal growth factor (rhEGF) and gentamicin sulfate at the wound site to hasten the process of diabetic wound healing and inactivation of bacterial growth. The physicochemical characterization of the fabricated scaffolds was carried out using scanning electron microscopy (SEM) and X-ay diffraction (XRD). The scaffolds were analyzed for thermal stability using thermogravimetric analysis and differential scanning calorimetry. The porosity, biodegradability, and swelling behavior of the scaffolds was also evaluated. Encapsulation efficiency, drug loading capacity, and in vitro drug release were also investigated. Further, the bacterial inhibition percentage and detailed in vivo biocompatibility for wound healing efficiency was performed on diabetic C57BL6 mice with dorsal wounds. The scaffolds exhibited excellent wound healing and continuous proliferation of cells for 12 days. These results support the applicability of such systems in rapid healing of diabetic wounds and ulcers. View Full-Text
Keywords: tissue engineering; growth factor; diabetic; wound healing; nanocomposite tissue engineering; growth factor; diabetic; wound healing; nanocomposite
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Dwivedi, C.; Pandey, H.; Pandey, A.C.; Patil, S.; Ramteke, P.W.; Laux, P.; Luch, A.; Singh, A.V. In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing. Pharmaceutics 2019, 11, 180.

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