Doxorubicin-Loaded Delta Inulin Conjugates for Controlled and Targeted Drug Delivery: Development, Characterization, and In Vitro Evaluation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Synthesis of Doxorubicin-Loaded Microparticulate Inulin (MPI)
Modification of MPI Particles with Succinic Anhydride
Doxorubicin Attachment to MPI-Succinate Particles
2.2.2. Physicochemical Characterization of Doxorubicin-Loaded MPI
FTIR
NMR
SEM
2.2.3. Determination of Doxorubicin Loading
2.2.4. In Vitro Cleavage of MPI
Preparation of Artificial Lysosomal Fluid (ALF) and Simulated Body Fluid (SBF)
Cleavage of MPI
2.2.5. Study of Doxorubicin Release from MPI
Validation of the Analytical Method
Sample Preparation and Data Analysis
2.2.6. Cell Culture Conditions
2.2.7. Cellular Uptake of Doxorubicin
Cell Internalization Observations
Flow Cytometry Measurements
2.2.8. In Vitro Cytotoxicity Assay
3. Results/Discussion
3.1. Synthesis and Characterization of MPI-Doxorubicin
3.1.1. Analysis of MPI-Doxorubicin Using FTIR and NMR
Optimization of Doxorubicin Attachment to MPI
3.1.2. Size and Morphology by SEM
3.2. In Vitro Hydrolysis of MPI
3.3. Doxorubicin Loading and Release Evaluations
3.3.1. Analytical Method Development and Validation
3.3.2. Percentage of Drug Loading Determination
3.3.3. Release Profiles of Doxorubicin-Loaded MPI in the Different Release Medium
3.4. Cellular Uptake of MPI-Doxorubicin by Monocytes Cells
3.5. In Vitro Cytotoxicity
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, L.; Song, Y.; Parikh, A.; Joyce, P.; Chung, R.; Liu, L.; Afinjuomo, F.; Hayball, J.D.; Petrovsky, N.; Barclay, T.G.; et al. Doxorubicin-Loaded Delta Inulin Conjugates for Controlled and Targeted Drug Delivery: Development, Characterization, and In Vitro Evaluation. Pharmaceutics 2019, 11, 581. https://doi.org/10.3390/pharmaceutics11110581
Wang L, Song Y, Parikh A, Joyce P, Chung R, Liu L, Afinjuomo F, Hayball JD, Petrovsky N, Barclay TG, et al. Doxorubicin-Loaded Delta Inulin Conjugates for Controlled and Targeted Drug Delivery: Development, Characterization, and In Vitro Evaluation. Pharmaceutics. 2019; 11(11):581. https://doi.org/10.3390/pharmaceutics11110581
Chicago/Turabian StyleWang, Lixin, Yunmei Song, Ankit Parikh, Paul Joyce, Rosa Chung, Liang Liu, Franklin Afinjuomo, John D. Hayball, Nikolai Petrovsky, Thomas G. Barclay, and et al. 2019. "Doxorubicin-Loaded Delta Inulin Conjugates for Controlled and Targeted Drug Delivery: Development, Characterization, and In Vitro Evaluation" Pharmaceutics 11, no. 11: 581. https://doi.org/10.3390/pharmaceutics11110581