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Viruses 2016, 8(12), 332;

Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China
College of Veterinary Medicine, Jilin University, Changchun 130062, China
State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China
Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Authors to whom correspondence should be addressed.
These authors contributed equally to this paper.
Received: 8 October 2016 / Accepted: 13 December 2016 / Published: 18 December 2016
(This article belongs to the Special Issue Advances in Flavivirus Research)
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West Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study, we prepared and evaluated the protective efficacy of immune serum IgG and pepsin-digested F(ab′)2 fragments from horses immunized with the WNV virus-like particles (VLP) expressing the WNV M and E proteins. Immune equine F(ab′)2 fragments and immune horse sera efficiently neutralized WNV infection in tissue culture. The passive transfer of equine immune antibodies significantly accelerated the virus clearance in the spleens and brains of WNV infected mice, and reduced mortality. Thus, equine immunoglobulin or equine neutralizing F(ab′)2 passive immunotherapy is a potential strategy for the prophylactic or therapeutic treatment of patients infected with WNV. View Full-Text
Keywords: West Nile virus; equine immunoglobulin; F(ab′)2 fragments; mice West Nile virus; equine immunoglobulin; F(ab′)2 fragments; mice

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Cui, J.; Zhao, Y.; Wang, H.; Qiu, B.; Cao, Z.; Li, Q.; Zhang, Y.; Yan, F.; Jin, H.; Wang, T.; Sun, W.; Feng, N.; Gao, Y.; Sun, J.; Wang, Y.; Perlman, S.; Zhao, J.; Yang, S.; Xia, X. Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection. Viruses 2016, 8, 332.

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