Enhanced Replication of Hepatitis E Virus Strain 47832c in an A549-Derived Subclonal Cell Line
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Generation of Clonal Cell Lines from A549 Cell Culture
2.3. Virus Stock Preparation
2.4. HEV Inoculation of Cell Cultures
2.5. RT-qPCR
2.6. Immunofluorescence Test
2.7. Transcriptome Analysis
2.8. Antibody Blocking Assay
3. Results
3.1. Inoculation of Different Cell Lines with HEV Strain 47832c
3.2. Generation and HEV Inoculation of A549-Derived Subclonal Cell Lines
3.3. Transcriptome Analysis of Cells
3.4. Effect of Anti-CEACAM and Anti-SDC2 Antibodies on HEV Replication in A549/D3 and A549 Cells
4. Discussion
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Probe ID | Gene Symbol | Gene Name | Fold Change |
---|---|---|---|
Upregulated | |||
17071144 | SDC2 | Syndecan 2 | 11.005 |
17107867 | MAGEA6/3 | Melanoma antigen family A 6 and 3 | 8.843 |
17077525 | CA8 | Carbonic anhydrase VIII | 7.308 |
17023338 | RNA5SP216 | RNA, 5S ribosomal pseudogene 216 | 7.219 |
16948063 | NLGN1 | Neuroligin 1 | 6.374 |
Downregulated | |||
16862563 | CEACAM6 | Carcinoembryonic antigen-related cell adhesion molecule 6 | −50.609 |
16872621 | miscellaneous | Chromosome (Chr) 19: 42219580–42223939 | −23.484 |
16862548 | CEACAM5 | Carcinoembryonic antigen-related cell adhesion molecule 5 | −21.642 |
16738803 | TCN1 | Transcobalamin I | −18.276 |
16879863 | EPCAM | Epithelial cell adhesion molecule | −13.057 |
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Schemmerer, M.; Apelt, S.; Trojnar, E.; Ulrich, R.G.; Wenzel, J.J.; Johne, R. Enhanced Replication of Hepatitis E Virus Strain 47832c in an A549-Derived Subclonal Cell Line. Viruses 2016, 8, 267. https://doi.org/10.3390/v8100267
Schemmerer M, Apelt S, Trojnar E, Ulrich RG, Wenzel JJ, Johne R. Enhanced Replication of Hepatitis E Virus Strain 47832c in an A549-Derived Subclonal Cell Line. Viruses. 2016; 8(10):267. https://doi.org/10.3390/v8100267
Chicago/Turabian StyleSchemmerer, Mathias, Silke Apelt, Eva Trojnar, Rainer G. Ulrich, Jürgen J. Wenzel, and Reimar Johne. 2016. "Enhanced Replication of Hepatitis E Virus Strain 47832c in an A549-Derived Subclonal Cell Line" Viruses 8, no. 10: 267. https://doi.org/10.3390/v8100267