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Article

Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach

by
Mohamed A. Lebdah
1,
Amal A. M. Eid
1,*,
Reham M. ElBakrey
1,
Abd Elgalil. El-Gohary
2,
Mohamed G. Seadawy
3,4,
Mohamed R. Mousa
5,
Hagar F. Gouda
6,
Nehal I. A. Goda
7,
Mostafa F. El-Hosseny
3,
Ahmed S. El-tahlawy
8,
Rokayya Sami
9,
Rasha A. Al-Eisa
10 and
Sarah S. Helal
1,*
1
Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt
2
Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33511, Egypt
3
Biodefense Center for Infectious and Emerging Diseases, Ministry of Defense, Cairo 11775, Egypt
4
Faculty of Biotechnology, October University for Modern Sciences & Arts (MSA University), 6th of October City 12451, Egypt
5
Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt
6
Department of Animal Wealth Development (Biostatistics Subdivision), Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt
7
Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt
8
Food Hygiene, Safety, and Technology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt
9
Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
10
Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
*
Authors to whom correspondence should be addressed.
Viruses 2025, 17(1), 96; https://doi.org/10.3390/v17010096
Submission received: 17 November 2024 / Revised: 22 December 2024 / Accepted: 30 December 2024 / Published: 13 January 2025
(This article belongs to the Special Issue Advances in Parvovirus Research 2024)
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Abstract

The present study aims to better understand the nature of currently circulating GPV strains and their pathological impact on the immune system during natural outbreaks among different duck breeds in Egypt. For this purpose, 99 ducks (25 flocks) of different breeds, aged 14–75 days, were clinically examined, and 75 tissue pools from the thymus, bursa of Fabricius, and spleen were submitted for virus detection and identification. Clinical and postmortem findings were suggestive of GPV infection. Concerning the immune system organs, atrophy in the thymus (60.6%), bursa (45.5%), and spleen (38.3%) was the most common gross lesion. Microscopically, the pathological impact of the virus was exhibited by a necrotic thymic cortex with Hassall’s corpuscle disintegration, the disappearance of normal bursal histological morphology accompanied by atrophied follicles and lymphocytic depletion, and apoptosis of B-lymphocytes in lymphoid follicles of the spleen. Furthermore, immunohistochemical examination revealed positive signals of the parvovirus detected in thymic lymphocytes in the cortex, bursa-dependent lymphoid follicle of the medulla, and diffuse positive expression of viral antigens in the spleen. GPV was detected in ducks using polymerase chain reaction, with the highest percentage of positive detection in the bursa of Fabricius (76%). Next-generation sequencing and phylogenetic analysis revealed that the detected virus was a variant of GPV, globally named novel GPV (NGPV), and closely related to Chinese NGPV isolates. To our knowledge, the current study is pioneering to address the immunopathological impact of NGPV among naturally infected ducks confirmed with full genome sequencing and immunohistochemical identification worldwide.
Keywords: novel goose parvovirus; immunohistochemistry; multiple correspondence; hierarchical clustering; next-generation sequencing; Egypt; immune system organs; ducks novel goose parvovirus; immunohistochemistry; multiple correspondence; hierarchical clustering; next-generation sequencing; Egypt; immune system organs; ducks

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MDPI and ACS Style

Lebdah, M.A.; Eid, A.A.M.; ElBakrey, R.M.; El-Gohary, A.E.; Seadawy, M.G.; Mousa, M.R.; Gouda, H.F.; Goda, N.I.A.; El-Hosseny, M.F.; El-tahlawy, A.S.; et al. Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach. Viruses 2025, 17, 96. https://doi.org/10.3390/v17010096

AMA Style

Lebdah MA, Eid AAM, ElBakrey RM, El-Gohary AE, Seadawy MG, Mousa MR, Gouda HF, Goda NIA, El-Hosseny MF, El-tahlawy AS, et al. Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach. Viruses. 2025; 17(1):96. https://doi.org/10.3390/v17010096

Chicago/Turabian Style

Lebdah, Mohamed A., Amal A. M. Eid, Reham M. ElBakrey, Abd Elgalil. El-Gohary, Mohamed G. Seadawy, Mohamed R. Mousa, Hagar F. Gouda, Nehal I. A. Goda, Mostafa F. El-Hosseny, Ahmed S. El-tahlawy, and et al. 2025. "Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach" Viruses 17, no. 1: 96. https://doi.org/10.3390/v17010096

APA Style

Lebdah, M. A., Eid, A. A. M., ElBakrey, R. M., El-Gohary, A. E., Seadawy, M. G., Mousa, M. R., Gouda, H. F., Goda, N. I. A., El-Hosseny, M. F., El-tahlawy, A. S., Sami, R., Al-Eisa, R. A., & Helal, S. S. (2025). Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach. Viruses, 17(1), 96. https://doi.org/10.3390/v17010096

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