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Article

Detection, Characterization and Sequencing of BTV Serotypes Circulating in Cuba in 2022

by
Ana María Acevedo
1,†,
Lydie Postic
2,†,
Maray Curiel
1,
Mathilde Gondard
2,
Emmanuel Bréard
2,
Stéphan Zientara
2,
Fabien Vorimore
3,
Mai-Lan Tran
3,
Mathilde Turpaud
2,
Giovanni Savini
4,
Alessio Lorusso
4,
Maurilia Marcacci
4,
Damien Vitour
2,
Pascal Dujardin
2,
Carmen Laura Perera
1,
Cristian Díaz
1,
Yalainne Obret
1 and
Corinne Sailleau
2,*
1
National Center for Animal and Plant Health (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas, San José de las Lajas 32700, Cuba
2
ANSES/INRAE/ENVA-UPEC, UMR 1161 Virology, Laboratoire de santé animale, 14 rue Pierre et Marie Curie, 94700 Maisons Alfort, France
3
Genomics Platform IdentyPath, Laboratory for Food Safety, ANSES, 94700 Maisons-Alfort, France
4
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Viruses 2024, 16(1), 164; https://doi.org/10.3390/v16010164
Submission received: 5 December 2023 / Revised: 19 January 2024 / Accepted: 19 January 2024 / Published: 22 January 2024
(This article belongs to the Special Issue Culicoides-Borne Viruses 2023)
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Abstract

In Cuba, despite a high sero-prevalence of bluetongue virus (BTV), circulating serotypes remain unknown. The aim of this study was to identify circulating BTV serotypes in farms throughout the western region of Cuba. Blood samples were collected from 200 young cattle and sheep between May and July 2022 for virological analyses (PCR, viral isolation and virus neutralization) and genome sequencing. The results confirmed viral circulation, with viro-prevalence of 25% for BTV. The virus was isolated from 18 blood samples and twelve BTV serotypes were identified by sequencing RT-PCR products targeting the segment 2 of the BTV genome (BTV-1, 2, 3, 6, 10, 12, 13, 17, 18, 19, 22 and 24). Finally, the full genome sequences of 17 Cuban BTV isolates were recovered using a Sequence Independent Single Primer Amplification (SISPA) approach combined to MinION Oxford Nanopore sequencing technology. All together, these results highlight the co-circulation of a wide diversity of BTV serotypes in a quite restricted area and emphasize the need for entomological and livestock surveillance, particularly in light of recent changes in the global distribution and nature of BTV infections.
Keywords: BTV; serotypes; PCR; isolation; Sanger; Nanopore sequencing BTV; serotypes; PCR; isolation; Sanger; Nanopore sequencing

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MDPI and ACS Style

Acevedo, A.M.; Postic, L.; Curiel, M.; Gondard, M.; Bréard, E.; Zientara, S.; Vorimore, F.; Tran, M.-L.; Turpaud, M.; Savini, G.; et al. Detection, Characterization and Sequencing of BTV Serotypes Circulating in Cuba in 2022. Viruses 2024, 16, 164. https://doi.org/10.3390/v16010164

AMA Style

Acevedo AM, Postic L, Curiel M, Gondard M, Bréard E, Zientara S, Vorimore F, Tran M-L, Turpaud M, Savini G, et al. Detection, Characterization and Sequencing of BTV Serotypes Circulating in Cuba in 2022. Viruses. 2024; 16(1):164. https://doi.org/10.3390/v16010164

Chicago/Turabian Style

Acevedo, Ana María, Lydie Postic, Maray Curiel, Mathilde Gondard, Emmanuel Bréard, Stéphan Zientara, Fabien Vorimore, Mai-Lan Tran, Mathilde Turpaud, Giovanni Savini, and et al. 2024. "Detection, Characterization and Sequencing of BTV Serotypes Circulating in Cuba in 2022" Viruses 16, no. 1: 164. https://doi.org/10.3390/v16010164

APA Style

Acevedo, A. M., Postic, L., Curiel, M., Gondard, M., Bréard, E., Zientara, S., Vorimore, F., Tran, M.-L., Turpaud, M., Savini, G., Lorusso, A., Marcacci, M., Vitour, D., Dujardin, P., Perera, C. L., Díaz, C., Obret, Y., & Sailleau, C. (2024). Detection, Characterization and Sequencing of BTV Serotypes Circulating in Cuba in 2022. Viruses, 16(1), 164. https://doi.org/10.3390/v16010164

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