Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Virus
2.2. Cell Culture and Treatment
2.2.1. In Vitro Cytotoxicity Assay
2.2.2. In Vitro Infection Inhibition Assay
2.2.3. Preparation of Positive Standard
2.3. ELISA
2.4. qRT-PCR
2.5. Western Blot
2.6. Statistical Analysis
3. Results
3.1. Maximum Nontoxic Concentration Determination
3.2. Virus Inhibition Assay
3.3. Effect of AS-IV on Innate Immune Function after PRRSV Infection
3.4. AS-IV Regulates the cGAS-STING Signaling Pathway after PRRSV Infection
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Sequence (5′—3′) |
---|---|
GAPDH-F | TGACATCAAGAAGGTGGTGAAGCAG |
GAPDH-R | GTGTCGCTGTTGAAGTCAGAGGAG |
cGAS-F | CTTTCACCTATGTGCCGACAACCC |
cGAS-R | TGTCACGCAGTTATCAAAGCAGAGG |
TBK1-F | AAGCCTTCTGGTGCAATATCTGGAG |
TBK1-R | ACCTGAAGACCCCGAGAAAGACTG |
IRF-3-F | GAGGCTCGTGATGGTCAAGGTTG |
IRF-3-R | AGTGGGTGGCTGTTGGAAATGTG |
IFN-F | GAGTGTGGAGACCATCAAGGAAGAC |
IFN-R | GTTCATGTACTGCTTTGCGTTGGAC |
OAS1-F | GCGAGTTCTCCACCTGCTTCAC |
OAS1-R | ACTAGGCGGATGAGGCTCTTGAG |
ISG15-F | GGTGGTGGACAGATGCGATGAAC |
ISG15-R | GGCTCACTTGCTGCTTCAGGTG |
SLA-1-F | GGATGAGGAGACGCGGAAAGTCA |
SLA-1-R | TGGTCCCAAGTAGCAGCCAAACA |
SLA-DRB1-F | CGACTTTGACCCGCAGAATGG |
SLA-DRB1-R | TGGTGGCTCTGGTATGGTTGGA |
CD86-F | CTCTTTGTGATGGTCCTCCTG |
CD86-R | AGGCTTAGGTTCTGCGAGTT |
CD80-F | GGGAACACCATTACCCAAGC |
CD80-R | GTCACCTGAACGATGCCTGA |
Nramp1-F | GTCTCCTTCTTCATCAACCTCTT |
Nramp1-R | ATCACGCCGCCTTGGTAA |
SP-A-F | GGAGACTTCTTCTACTTGGATGG |
SP-A-R | GCTGGCAGTTCCTGTCATTC |
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Song, K.; Yu, J.-Y.; Li, J.; Li, M.; Peng, L.-Y.; Yi, P.-F. Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection. Viruses 2023, 15, 1586. https://doi.org/10.3390/v15071586
Song K, Yu J-Y, Li J, Li M, Peng L-Y, Yi P-F. Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection. Viruses. 2023; 15(7):1586. https://doi.org/10.3390/v15071586
Chicago/Turabian StyleSong, Ke, Jia-Ying Yu, Jiang Li, Miao Li, Lu-Yuan Peng, and Peng-Fei Yi. 2023. "Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection" Viruses 15, no. 7: 1586. https://doi.org/10.3390/v15071586
APA StyleSong, K., Yu, J.-Y., Li, J., Li, M., Peng, L.-Y., & Yi, P.-F. (2023). Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection. Viruses, 15(7), 1586. https://doi.org/10.3390/v15071586