Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping
Abstract
:1. Introduction
2. Materials and Methods
2.1. B Cell Epitope Prediction
2.2. Preparation of Recombinant PEDV S Protein
2.3. Immunization of Animals
2.4. Biosynthesis of 16-Mer Peptides
2.5. SDS-PAGE and WB
2.6. Immunofluorescence Assay (IFA)
2.7. Immunohistochemistry Assay (IHC)
3. Results
3.1. Prediction of BCEs on SCV777 and SSD2014
3.2. Reactivity of Acquired Anti-S Sera with PEDV DR13
3.3. Reactivity of Anti-S Sera with SCV777 and SSD2014
3.4. Identification of Positive 16-Mers on SCV777
3.5. Identification of Positive 16-Mers on SSD2014
3.6. An Overview of Positive 16-Mers on SCV777 and SSD2014
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SCV777 | SSD2014 | ||||||
---|---|---|---|---|---|---|---|
No. | Position | Sequences | Antigenicity a | No. | Position | Sequences | Antigenicity |
E1 | 19–30 | LPQDVTRCQSTIN | 0.0361 | E1 | 21–28 | QDVTRCSA | −0.0665 |
E2 | 52–62 | AVVVLGGYLPSMNSSSWY | 0.1657 | E2 | 55–64 | IGENQGVNST | 0.3703 |
E3 | 65–72 | TGIETDSG | 0.3072 | E3 | 70–76 | QHPTASG | 0.9738 |
E4 | 91–100 | ISQEPFDPSG | 0.6991 | E4 | 95–105 | ISQEPFDPSGY | 0.6324 |
E5 | 102–110 | QLYLHKATN | −0.3804 | ||||
E5 | 111–120 | KATNGNTNAT | 0.4115 | ||||
E6 | 125–142 | QLYLHKATNGNTSAIARLRICQFPDNKTLGPTVNDVTTGRN | 0.3121 | E6 | 133–146 | TLGPTANNDVTTGR | 1.0705 |
E7 | 186–192 | SRVATRC | 2.1198 | E7 | 188–194 | DWSRVAT | 0.5834 |
E8 | 195–202 | KRSCAMQY | 0.2537 | ||||
E9 | 215–223 | SAGEDGIYY | −0.0055 | E8 | 219–233 | SAGEDGISYQPCTAN | 0.0702 |
E10 | 226–232 | CTANCSG | −0.4497 | ||||
E11 | 238–248 | FATDSNGHIPE | −0.7913 | E9 | 244–253 | TEPNGHIPEG | −0.7045 |
E12 | 297–310 | QTMDGVCNGAAAQR | 0.4745 | E10 | 301–307 | QTIDGVC | 1.0282 |
E13 | 347–353 | NSSDPHK | 0.2269 | E11 | 350–356 | SNSSNPH | 0.4187 |
E14 | 372–378 | KVDTYKS | −1.422 | ||||
E15 | 423–432 | TGHGTDDDVS | 0.5537 | E12 | 427–436 | TGHGTDDDVS | 0.5537 |
E16 | 461–475 | DDPVSQLKCSQVSFD | 0.8676 | E13 | 465–473 | DDPVSQLKC | 0.4517 |
E17 | 483–490 | ISSRNLLS | 0.4671 | ||||
E14 | 520–526 | SFGGHSG | 0.4737 | ||||
E18 | 528–535 | SDTTINGF | −0.3571 | E15 | 530–539 | IASDTTINGF | −0.4022 |
E19 | 555–573 | NSYGYVSKSQDSNCPFTLQ | 0.8752 | E16 | 559–575 | NSYGYVSKSQDSNCPFT | 0.7085 |
E17 | 608–613 | EFGSGV | 0.6906 | ||||
E20 | 622–634 | ELITGTPKPLQGV | 0.1569 | E18 | 627–640 | LITGTPKPLEGVTD | 0.2387 |
E21 | 688–694 | GAVYSVT | 0.0153 | ||||
E22 | 716–728 | LSNSTFNNTRELP | 0.4482 | E19 | 720–732 | LSSSTFNSTRELP | 0.2311 |
E23 | 734–744 | SNDGSNCTEPV | 0.3639 | E20 | 738–747 | SNDGSNCTEP | 0.4006 |
E21 | 762–775 | IGYVPSQSGQVKIA | 0.8513 | ||||
E24 | 800–817 | VSVDCVTYVCNGNSRCKQ | 0.8176 | E22 | 803–819 | PVSVDCATYVCNGNSRC | 0.5376 |
E25 | 860–869 | ISSFNGDGYN | −0.77 | E23 | 865–873 | SSFNGDGYN | −0.7927 |
E26 | 875–887 | GVSVYDPASGRVV | 0.5215 | E24 | 879–893 | GVSVYDPASGRVVQR | 0.6336 |
E27 | 904–921 | NGLGTVDEDYKRCSNGRS | 0.2071 | E25 | 906–925 | VTNGLGTVDEDYKRCSNGRS | 0.2239 |
E28 | 1013–1024 | VKEAISQTSNGL | 0.0891 | E26 | 1017–1030 | VKEAISQTSKGLNT | 0.3561 |
E29 | 1037–1043 | VVNSQGS | −0.0001 | ||||
E27 | 1064–1070 | SSSIDDI | 0.4527 | ||||
E30 | 1118–1131 | CVKSQSQRYGFCGG | 0.6518 | E28 | 1101–1135 | TLTKYTEVQASRKLAQQKVNECVKSQSQRYGFCGG | 0.2232 |
E31 | 1205–1214 | MFEPRKPTVS | 0.4124 | E29 | 1194–1218 | LQNHTATEYFVSSRRMFEPRKPTVS | 0.0433 |
E32 | 1230–1243 | TSDQLPDVIPDYID | 0.4996 | E30 | 1233–1246 | LTRDQLPDVIPDYI | 0.3167 |
E33 | 1256–1262 | PNRIGPS | 1.1446 | E31 | 1259–1267 | LPNRTGPSL | 0.9542 |
E34 | 1280–1297 | ADLEQRSESLRNTTEELR | 0.615 | E32 | 1284–1300 | ADLEQRSESLRNTTEEL | 0.5571 |
E35 | 1348–1373 | TGCCGCCGCCGACFSGCCRGPRLQPY | −0.2869 | E33 | 1351–1361 | STGCCGCCGCC | −0.5985 |
E34 | 1364–1376 | CFSGCCRGPRLQP | 0.1851 |
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Yu, R.; Dong, S.; Chen, B.; Liu, Y.; Li, F.; Si, F.; Xie, C.; Li, Z. Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping. Viruses 2022, 14, 1371. https://doi.org/10.3390/v14071371
Yu R, Dong S, Chen B, Liu Y, Li F, Si F, Xie C, Li Z. Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping. Viruses. 2022; 14(7):1371. https://doi.org/10.3390/v14071371
Chicago/Turabian StyleYu, Ruisong, Shijuan Dong, Bingqing Chen, Yingjie Liu, Fengping Li, Fusheng Si, Chunfang Xie, and Zhen Li. 2022. "Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping" Viruses 14, no. 7: 1371. https://doi.org/10.3390/v14071371
APA StyleYu, R., Dong, S., Chen, B., Liu, Y., Li, F., Si, F., Xie, C., & Li, Z. (2022). Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping. Viruses, 14(7), 1371. https://doi.org/10.3390/v14071371